系统性红斑狼疮患者中TNF的检测

TNF:,(,),(,,):,100730,(E2mail:[e-mail])(systemiclupusery2thematosus,SLE)TNF2,sTNFRTNF2,SLETNFTNF2TNF2(,LT)MHCTNF,(,LT)TNF,1SLETNF2sTNFRLT:28SLE(37.2),1982SLE114(39.4);(PBL),PBLRNA11TNF2cDNATNFDNA,TNF2DNALT(L:5GCTGCGCAGCTCTCTGTAC3;R:5AAGAAG2GTCTTCCCTCTCGC3),TNFPCR240bp,DNALT21ELISATNF2()sTNFR&TNF2(R&DSys2tem)31PBLRNA(Molecular2cloningALaboratoryManual):RNA10g,232P2dCTPTNF2cDNA,,LTTNF2:tt11TNF25pgPml28SLE29TNF2,SLE(14.07.3pgPml),,3SLETNF24446.399.5pgPml,30pgPml,,TNF2(7.8pgPml)21TNF(sTNFR)&1sTNFR,SLEsTNFR&;2sTNFRSLE(P0.001)sTNFRTNF,TNF,sTNFRTNFTNF2RA,2sTNFRTNF2,SLE,sTNFRSLE31sTNFR&s(pgPml)P3(t)sTNFR289832540.001SLE2824571397sTNFR2520025340.001SLE286406202431PBLRNA2TNF2:SLETNF2:SLELT:SLE33220023222ChinJMicrobiolImmunol,March2002,Vol22,No.2,SLERNA,SLEPBLTNF2mRNA,SLETNF2,,mRNA,,2RNAsP3(tt)TNF23649647442320.01SLE1111146463744LT1493875516720.05SLE24158338109297:,105SLELT,,SLETNF2,TNF2,;SLEsTNFR,TNF21BeutlerB,vanHaffelC.UnravelingfunctionintheTNFligandandre2ceptorfamilies.Science,1994,264:6672668.2MorelandLW,HeckLWJr,KoopmanWJ,etal.Biologicagentsfortreatingrheumatoidarthritis.ArthRhem,1997,40:3972409.3AderkaD,WysenbeekA,EngelmannH,etal.Correlationbetweense2rumlevelofsolubletumornecrosisfactorreceptoranddiseaseactivityinsystemiclupuserythematosus.ArthritisRheum,1993,36:111121120.(:2001206219)121(ICAM21)mRNA,ICAM21mRNA(COPD)240(1999,7:4172419)10,:(2000):030001,(,,,);(,):,(E2mail:[e-mail])123456,,(RT2PCR),HPIAS21000ICAM21ICAM21mRNA1ICAM2110.41.0,3.70.4,(P0.05)ICAM2117.76.29.6%(P0.05)RT2PCRICAM21mRNA1,561(P0.01),ICAM21mRNAICAM21ICAM21mRNACOPD,,ICAM21ICAM21mRNA,2,,,COPDICAM21,(:2001212203)43220023222ChinJMicrobiolImmunol,March2002,Vol22,No.2