免疫组织化学技术（immunohistochemistry）

DAB701S-10023EMA4IgκλBBIgκ+/λ+Igκ+λ+bcl-290%bcl-2bcl-25678c-erB-2c-mycP53nm23EGFRPCNAKi-67Ki-67PCNAEGFR9106TB10CMVHPVHSVHBV11.15~101.21~2mlHankRPMI-1640500r/min5~10min1106/ml11.31500r/min10min1.41.52105~6/ml50100l1000r/min2min0.6~1cm2105l2h24Poly-L-Lysine()300000.5%110602.5g500ml0.25g2minAPES(3--)1:50APES20sAPESPHSigmaSantaCruz10mg/ml0.1%-1.5%0.01-0.15NaN3NaN3-20202841-37102282020