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12005()8:30-8:508:50-9:009:00-9:409:40-10:2010:20-10:40TeaBreak10:40-11:30211:30-12:001.2.3.12:00-13:30Lunch13:30-15:20(I)13:30-13:50REMOVALLEAD(II),CADMIUM(II),ANDMERCURY(II)IONSFROMAQUEOUSSOLUTIONBYLOOFASPONGEIOMMOBILIZEDBIOMASSOFASPERGILLUSTERRUES13:50-14:10STUDIESONVIBRIOPARAHAEMOLYTICUSBYPCRDURINGTHEFOURSEASONSINTHESOUTHCOASTOFTAIWAN14:10-14:4014:40-15:20(II)14:40-15:0015:00-15:2015:20-15:3032005…………………………………………………………………………..1…………………………………………………………………………..3P16P27P38P49P5104P611P712P813P914P1015P1116P1217P13Pb18P14Aspergillusterreus195/O1REMOVALLEAD(II),CADMIUM(II),ANDMERCURY(II)IONSFROMAQUEOUSSOLUTIONBYLOOFASPONGEIOMMOBILIZEDBIOMASSOFASPERGILLUSTERRUES21O2STUDIESONVIBRIOPARAHAEMOLYTICUSBYPCRDURINGTHEFOURSEASONSINTHESOUTHCOASTOFTAIWAN28O336O44220056P.1biogeochemicalcycleNaviculacryptotenellaMicrosystisaeruginosa7P.2SARSuraninesodiumfluoresceincoliphageCollisonnebulizerantifoamAwettingagentTween80peptoneAerodynamicParticleSizerSpectrometerAPSMarpleeight-stageMarplepersonalimpactorpolyvinylchloridefilter,PVCfilterMarple20ml1596microplate8P.31121212310254nm495.93µW/cm2220.75µW/cm222.510.50.9560.3820.191Joule0.9560.382JouleUVC0.956JouleUVC3~4ToxstatTMLD50LD505hrLD508hr0.700.16JouleLD50C9P.4(Curinggun)ACGIHUVUVC254nm30cm70.14µW/cm2ACGIH42.77UVUVUVUVUVCuringGunUVUVAUVUVC10P.51121B2::IncidentCommandSystemMitigationMSDS921129605941931-106386039825773%,,,,,,,:11P.611221B2::12P.7B(PolycyclicAromaticHydrocarbonsPAHs)PAHs2PAHsABPAHs200518~9NIOSHNo.5506XAD-2PAHs14PAHsAB0.240.12mg/m30.150.08mg/m3APAHs23.4320.91ng/m32111.611584.81ng/m32135.041575.50ng/m3B16.326.73ng/m32307.17832.98ng/m32323.49834.61ng/m3PAHsPAHs99PAHsPAHsPAHsPAHs13P.8AVOC2LPM3LPM14P.9121A2A()VOCACHACH15P.10B76~9120015010121012~22T-shit19.6(1.1)172.3(4.0)70.3(12.5)19.51F8F1681F8F8F1F78/82/2(MHW-150K)0kg6kg9kg15kg(PolarHeartRateMonitor)516P.1112312A3A17P.1211212pH(NaCl)pH18PbP.13111212AspergillusterreusAspergillusterreus19AspergillusterreusP.14(T.S.Wu)1(L.C.Chen)212AspergillusterreusCr6+Sn2+A.terreusCr6+Sn2+qmax144hqmax0.1mgofCr6+/gofbiomass8.89mgofSn2+/gofbiomassA.terreus2021REMOVALLEAD(II),CADMIUM(II),ANDMERCURY(II)IONSFROMAQUEOUSSOLUTIONBYLOOFASPONGEIOMMOBILIZEDBIOMASSOFASPERGILLUSTERRUESO.11212AAbstractThebiosorptionprocessfortheremovaloflead(II),cadmium(II),andmercury(II)ionsbyloofaspongeimmobilizedbiomassofAspergillusterrues(FBILS),anewlydevelopedimmobilizedbiosorbent.EffectsofenvironmentalfactorsonmetaluptakecapacityofFBILSwerestudied.Lead(II),cadmium(II),andmercury(II)ionsremovalbyFBILSwasfoundtobeinfluencedbypHofthesolution,initialmetalconcentration,temperature,androtationrate.FBILSwasfoundtoremoveheavymetalionsefficientlyfromaqueoussolutionwithselectivityintheorderofPb(II)Hg(II)Cd(II).ThebiosorptionofmetalionsbyFBILSincreasedastheinitialconcentrationofmetalionsincreasedinthemedium.Themaximumheavymetalionsadsorbedwasfoundtobe305.0,46.8,33.7mg/gFBILSforPb(II),Hg(II)andCd(II),respectively.ThemaximumuptakeofmetalionswasobtainedatpH3~5.Temperatureovertherange10~50hadnoeffectonthebiosorptioncapacityofFBILS.Duringthesebiosorptionstudies,FBILSexhibitedgoodphysicalandchemicalstability.Thekineticsoflead(II),andcadmium(II)removalwasextremelyfastreachingatequilibriuminabout30minforFBILS.TheresultssuggestthatFBILScanbeusedasabiosorbentforanefficientremovalofheavymetalionsfromaqueoussolution.Keywords:Biosorption,immobilization,Loofasponge,lead(II),Aspergillusterrues22232425262728STUDIESONVIBRIOPARAHAEMOLYTICUSBYPCRDURINGTHEFOURSEASONSINTHESOUTHCOASTOFTAIWANO.2AbstractVibrioparahaemolyticusisacommonfood-borneentericpathogeninAsia,occurringnaturallyinestuarineandmarineenvironmentsthroughouttheworld.Theincidenceofthisorganisminanaquaticenvironmentdependsuponmanyfactors.SeawaterandorganicmaterialwerecollectedduringthefourseasonsfromthesouthcoastofTaiwan,andanalyzedtodetermineV.parahaemolyticusdensitiesandtheoccurrenceofpathogenicstrains,definedasthosepossessingtdhand/ortrhgenesbypolymerasechainreaction(PCR),usingisolatedDNAfromenrichmentcultureofthesamples.About85.2%ofsampleswerepositiveforV.parahaemolyticuswithdensitiesof3to1100cellsper100mlofwateror10goforganicsamplesbythemost-probable-number(MPN)-PCRtechnique,butonly59.3%werepositivebytheconventionalMPNculturetechnique,withdensitiesrangingfrom3to1100cellsper100mlofwateror10goforganics.Furthermore,thetdhandtrhgeneswerepositivein7.4%and13.9%ofsamples,respectively,bytheMPN-PCRtechnique.Notdhand1.9%trhgene-positivestrainswereisolatedbytheconventionalMPNcultureprocedure.ThepercentageofO10serotypeoccupiedhighest.ThedifferenceindetectionbetweentheMPNcultureandtheMPN-PCRtechniquesappearedtobesignificantandmaybeattributedtodifferentdetectionsensitivitiesandotherfactors.Keywords:Vibrioparahaemolyticus,food-borneentericpathogen,PCR2930313233343536O.31212254nmC12310580.59µW/cm2220.75µW/cm22.02.510.51.090.440.22JouleUVC1.090.44Joule0.22JouleUVC1.09Joule80.44Joule90.22Joule11(0.22Joule)(1.09Joule)C[1,2,3][4][5,6][7]3720(Chlorellaspp.)25150ml[8,9]CSmedium1[10]1212hrLD,hr75rpm25OLYMPUSCH308mChlorellaspp.Chlorellaspp.1.C