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23520049JournalofWuxiUniversityofLightIndustryVol.23No.5Sep.2004:10092038X(2004)0520096205:2003210221;:2004203222.:(98JKZD006).:(19472),,,,.,,(,410128):,25,.,3%,8%,4%,16h.:;;;:TS25214:ATheManufacturingTechnologyofANew2TypeKefirZHOUChuan2yun,NIEMing,WANJia2rong(FacultyofFoodScienceandTechnology,HunanAgriculturalUniversity,Changsha410128,China)Abstract:Themanufacturingtechnologyofaddingfruitjuicetomilkforkefirproductionwasstudiedinthispaper.Thefruitkefirwasmanufacturedbyaddingstrawberryjuice,pineapplejuiceandcanesugartofreshmilk,inoculatingstarterwhichwasmadebykefirgrainsafterbactericidationandcooling,andthenfermentingat25.Thebestmanufacturingtechnologywasobtainedthroughorthogonalexperiment.Theresultshowedthat,thebesttaste,organizationstateandflavorofthenew2typekefircouldbeproducedbyadding3%strawberryjuiceandpineapplejuice,8%canesugar,4%starter,fermentingfor16h.Keywords:strawberryjuice;pineapplejuice;fermentation;kefir(Kefir).,,(Kefirgrains),CO2[1],[2],.(),,..[3],,.,,.,,;B;CO2;,CO2,[2,4]..10,,,,.11.11.1.1.1.1.2.1.1.3,60BX.1.1.4.1.1.5MRS[5],[6],[7],[7],[7],[7].1.1.6,250B,,(),SW2CJ2IB,,,.1.21.2.1150,253:(121,5min)26(150)(25,1214h)(810,12h),.1.2.2(70)()(45MPa)(9095,20min)()30(25)(68,12h).1.2.3L16(45)(1),25.1L16(45)Tab.1ThefactorleveloforthogonalexperimentsA/%)B/%C/%D/%E/h12121122324214343631645484181.2.4[2,3],,[7],.,MRS[5,6];[7];[7].:(106CFU/mL)(4%)(500mL,300mL),25,2h(T),[7];,.10%,,,2548h,,,.1.2.55.0g,150mL,40mL40,;,0.1mol/LNaOH(30s),NaOH,20(T)[8].3,.1.2.62[9],3,.795:22.11)(CFU/mL):1.2109;3.8106;1.4105;5.7105.2),2.2Tab.2Theresultoffermentingmilkbylactobacillus/h/T/T224204302363829849381056491258601459691659772,,;.3).4):60%,;35%,;5%,.2.2L16(45)6812h,252,,3.3Tab.3Theorganolepticevaluationstandardforproduct(40)(30)(30),,,354025302530,,,213416241624,,,,2015154,4,6,11.RI,5EDACB,.A2B3C4D4E3,()3%,3%,8%()4%,16h,25,.2.3F,16.4,RII,5CDBEA,,109.78T,24.78T.A2B3C4D4E3,C492.87T,,,.2.44RIII,5CAEBD,.5,C4117%,,(215%)68%,,.31),25.2)L16(45),,:,()3%,3%,8%()4%,16h.:;8923:.,,,,,,,.4L16(45)Tab.4TheresultoforthogonalexperimentsofL16(45)ABCDE/T/%11111161.424.780.621222273.561.970.731333385.986.871.541444493.298.982.252123478.530.630.862214391.768.790.772341273.572.851.482432177.683.711.193134271.794.761.2103243175.8109.781.4113312489.638.470.9123421381.927.150.8134142386.789.861.7144231455.863.471.4154324177.972.711.0164413257.663.251.2I1314298.3300.3272.6292.7I2321.3296.8311.8327.4276.3I3319326.9291.0297.8346.2I4278310.3329.2334.5317.1I1/478.574.5875.0868.1573.18I2/480.3374.2077.9581.8569.08I3/479.7581.7372.7574.4586.55I4/469.577.5882.386.6379.28RI10.837.539.5515.4817.47RI,5:EDACB1272.6240.06195.29188.25290.982255.98304.01232.48274.01292.833270.16270.9328.81290.53272.674289.29273.09371.5335.24231.551/468.1560.0248.8247.0672.752/464.0076.0058.1268.5073.213/467.5467.7382.2072.6368.174/472.3268.2792.8883.8157.89R8.3215.9844.0636.7515.32RII,5:CDBEA15004303404204102400420330440450343048052049047045305306705105301/4125107.585105102.52/410010582.5110112.53/4107.5120130122.5117.54/4132.5132.5167.5127.5132.5R32.527.58522.530RIII,5:CAEBD995::[1],,,.(kefir)[J].,1996,24(2):20-22.[2],,,.[M].:,1994.133-139.[3],,,.[J].,1996,24(4):39-42.[4].[M].:,2001.106-108.[5].[M].:,1992.[6],,,.[J].,2003,(1),26.[7],.[M].:,1997.110-178.[8]GB/T5009.41996,[S].1997.[9].[M].:,1998.(:)2005;,,,,16,56,10,5,60:4675,();:72(),:1469:200011:(021)63781733:(021)6377326400123