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26320096JOURNALOFNATURALSCIENCEOFHEILONGJIANGUNIVERSITYVol126No13June,2009,,,,,(,150040):,,(CCD)45,(RSA),:68%;111%;215h;10111(mLg)5168%,6173%:;;;:O69:A:1001-7011(2009)03-0390-06:2008-10-20:(2006BAD18B04):(1976-),,,,::(1954-),,,,0(Glycyrrhiza)(Leguminosae)[1],[2-3][4],,[5-7],,[8][9-10],,,,,(ResponseSurfaceMethodology,RSM)Box1957[11],[12-16],,,,1111(980,975,Jasco);UV-2550-();();();PW177();()(,Sigma);(98%,AcrosOrganics);(98%,:111610-200604,),,(GlycyrrhizauralensisFisch)(,),,60,11210100g(W0)500mL,,,,(L),1mL,(X1)(X2),12(Y1)(Y2):Y1=X1LW0100%(1)Y2=X2LW0100%(2)113[17],X1(mgmL-1)Y1:Y1=748861416956X1+12802015243,R2=019996,010074119000mgmL-1[18],X2(mgmL-1)Y2:Y2=015557X2+010210,R2=019987,010728213300mgmL-1221121111,5,,0153%;1,5,12243648h,0178%1134%:48h,,211123,,,,991361125%,21221211,,6,1121%;1,6,12243648h,1136%1172%:48h,,212.23,3,,,981891136%,213,-,10100g,,141933:1,,,70%,,,70%2,,,1%,110%112%,111%3,1155h,,215h,,,215h4,,,10mLg,,10(mLg)214,4,Design-Expert710,,,4530(6),1,21Table1Experimentalfactoroflevelandcode--101/%AX16065707580/%BX21100110511111151120/hCX32212521521753/(mLg)DX4991510101511:,X1=(A-70)/5,X2=(B-111)/0105,X3=(C-215)/0125,X4=(D-10)/0152Table2Centralcompositedesignandexperimentalresults/%/%/%/%X1X2X3X4X1X2X3X4111-114180510916-11-11517151132111-141965112171-1-1-1510341453-1-1-1-1510151291811-1-14159515841-11151114187191111416551225-1-1-1151706181201-11-1512741606-1-11-14183519721-000414051347000061056199220000515861488-11115129519723000314741339-111-151326170240005105419510-1-1115107515725000-41234163110000518761782600041765168120000519561442700005133614413-11-1-141894186280-0041505109141-1-11516451552900-04183415115000051976174300004157418129326215Design-Expert7102,:Y=0+ixi+ijxixj+iixi2Y,xixj,0iiiij,3,,:Y1=516897-011515X1+011559X4-011658X1X2-010859X1X4+010842X2X3-011622X3X4-013573X12-012083X22-011423X32-011817X42:Y2=615694-013266X1+012154X2X3-012094X2X4-013687X12-013398X22-013044X32-013796X423Table3VarianceanalysisforregressionequationY1/%FPY2/%FP0150342410334010001331112568101040100023351696157X1-0115015512261315001000233-0133215598181216001000833X2-01050107213144010108488133E-041167E-051119E-04019915X3-01040104212100930117820115015442318728010692X40116015831271841401000133011012181115522012333X1X2-01170143992110030010004330116013963218200011153X1X30105010329115685012309-0119015558319550010666X1X4-0109011182516417010324301026181E-034184E-02018290X2X30108011134514143010355301220174225128160103753X2X4-0104010280113355012672-01210170144199140104233X3X4-0116014209201094701000533-012016186414020010545X12-01363150171671186801000133-0137317284261532301000133X22-0121111901561822201000133-0134311672221539101000333X32-0114015554261518801000133-013215421181090701000833X42-0118019053431224601000133-0138319516281120601000133010209011405010245210371012571011814417182010741010120010384:33(P0101);3(P0105)()(),F,P(ProbF),3,3P012571,0107410105,,3,X1X4X1X2X1X4X2X3X3X4X12X22X32X42;X1X2X3X2X4X12X22X32X42,,;,,;565,6,,;(2163h),3933:787,,8216,,,:68129%;1110%;2151h;101131,5173%,6165%,68%;111%;215h;10111,5168%6173%,,217,:(1),[19];,(2),,;,493263,,,,,,,,,[1].(,)[M].:,1998:167-175.[2],,.[M].:,1997:1256-1279.[3].[J].,2000,17(3):59-63.[4]HATANOT,TAKAGIM,ITOH,etal.AcylatedflavonoidGlycosidesandaccompanyingphenolicsfromLicorice[J].Phytochemistry,1998,47(2):287-293.[5]LINJC.MechanismofactionofglycyrrhizicacidininhibitionofEpstein-Barrvirusreplicationinvitro[J].AntiviralResearch,2003,59(1):41-47.[6]HARAGUCHIH,ISHIKAWAH,MIZUTANIK,etal.AntioxidativeandsuperoxidescavengingactivitiesofretrochalconesinGlycyrrhizainflata[J].BioarganicandMedicinalChemistry,1998,6(3):339-347.[7]HARAGUEHIH,TANIMOTOK,TAMURAY,etal.ModeofantibacterialactionofretochalconesfromGlycyrrhizainflate[J].Phytochemistry,1998,48(l):125-129.[8]LIW,ASADAY,YOSHIKAWAT.FlavonoidconstituentsfromGlycyrrhizaglabrahairyrootcultures[J].Phytochemistry,2000,55(5):447-456.[9],,.[J].,2006,21(2):12-13.[10],,,.[J].(),2007,28(2):35-37.[11]BOXGEP.Evolutionaryoperation:Amethodforincreasingindustrialproductivity[J].AppliedStatistics,1957,6(2):81-101.[12],,,.[J].,2007,12(5):52-56.[13],,.[J].,2003,20(1):118-121.[14],,,.[J].,2008,8(1):49-54.[15],,.[J].,2001,18(4):105-108.[16],,,.[J].,2008,36(7):185-190.[17]KOGAK,OHMACHIK,KAWASHIMAS,etal.Determinationof18-glycyrrhizinand18-glycyrrhizinindogplasmabyhigh-performanceliquidchromatography[J].JournalofChromatographyB,2000,738(1):165-168.[18],,,.[J].,2005,25(4):469-472.[19],.[J].,2007,18(3):648-650.OptimizationofcombinedextractionofglycyrrhizicacidandflavonoidsbyresponsesurfacemethodologyLIChun2ying,LIXiao2juan,YANGLei,ZHAOChun2jian,LIJia2hui,ZUYuan2gang(KeyLaboratoryofForestPlantEcology,MinistryofEducation,NortheastForestryUniversity,Harbin150040,China)Abstract:Responsesurfaceanalysismethodology(RSM)wasusedforoptimizingtheextractionprocessofglycyr2rhizicacidandflavonoidsfromglycyrrhiza.Basedonsingle2factorexperiments,threeindependentvariables,refluxsolvent,timeandratioofliquidtosolidwereselectedasaffectingfactorsduringextraction.Anexpevimentaldesignof4f