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©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(11,100700;21,110001)[]:,,:,HPLC:,,;,:,,[];;[]R28313[]A[]100125302(2006)1120889203[]2005203224[]3,Tel:(010)6401441122975PaeonialactifloraPall1,,,,[124],,,,,,1Waters600,996,Millennium2010,(HPLC),(07362200219)CCFG1-15();TM902C(,)()2211:(),36h,,,,:(1,115kW2):10%,15%,20%,,,,250g(1800g,,600g),,11/kW///min11.519785714321.519582616831.519680619341.519882617851.520083611361.015370016171.0153651110081.0153661011891.0193679120101.0197689155212(),,,,,988311120066ChinaJournalofChineseMateriaMedicaVol131,Issue11June,2006©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(416mm250mm,5m),25,22(3367012),1mLmin-111HPLCA1;B1;11:10L,5,,RSD112%:2,4,6,8,10L,,(g)(X),(Y),,Y=11621106X+21503104,,011380169g(r=019999):,0,2,4,8,12,24,48h,RSD210%,48h:5,,RSD214%:5,,,9910%,RSD213%:015g,,20mL,18mL,4h,30min,,,,,5L,2213122000[1]22/kW/%/min/%/%11.0127:015.10.4921.5129:014.90.6131.51211:014.50.5641.5207:015.80.5051.0209:014.30.5061.02011:015.30.5171.5507:015.40.5381.0509:014.70.5791.55011:014.30.57101.0127:014.60.473(),(),(8214118)(6712119),(19712119)(169182311),(1),(3)(P0105),,31.000.681.000.980.691.00-0.95-0.83-0.921.002,3,,,(P0105),[][1].,:.19791291[2]..:,1983167.[3]..:,1983142.[4]112000.43.098311120066ChinaJournalofChineseMateriaMedicaVol131,Issue11June,2006©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(11InstituteofChineseMateriaMedica,ChinaAcademyofTraditionalChineseMedicine,Beijing100700,China;21SchoolofMedicine,ChinaMedicalUniversity,Shenyang110001,China)[Abstract]Objective:ToprovideexperimentaldataforthequalitycontrolofprocessedPaeonialactiflora,aChineseherbalmedicine.Method:TraditionalprocessingofP.lactiflorawassimulated,contentofpaeoniflorinandwaterextractsamongdifferentprepara2tionswereassayedbyHPLC;Thequantitativecorrelationsamongdifferentprocessingconditionswereanalyzed,theeffectsofprocessingparam2etersonthecontentsofpaeoniflorinandwaterextractswereassayedandanalysed.ResultandConclusion:Thecontrolledprocessingparame2terswerecorrelatedwithcovariableswhichshowedthatprocessingprocedureswascontrollable,andtheheatingtemperaturewasafactorim2pactingthecontentofpaeoniflorin.[Keywords]Paeonialactiflora;paeoniflorin;traditionalChinesepharmaceuticalprocessing[](883)HPLCdeterminationofcaffeicacidinHerbaLycopiNIEBo1,LIUYong1,XUQing2,SHIJin2li1,LIANGXin2miao2,XIAOPei2gen3(11SchoolofChinesePharmacy,BeijingUniversityofChineseMedicine,Beijing100102,China;21DalianInstitueofChemicalPhysics,ChineseAcademyofSciences,Dalian116012,China;31InstituteofMedicinalPlant,PekingUnionMedicalCollege,ChineseAcademyofMedicalSciences,Beijing100094,China)[Abstract]Objective:TodevelopanewmethodfordeterminationofcaffeicacidinHerbaLycopibyHPLCandanalyzethesamplesfromdifferenthabitatsandcollectingtime.Method:Thesampleswereextractedwith30%ethanolbyultrasonic.SeparationwascarriedoutonaHypersilODS2columnusingCH3CN2012%H3PO4(1189)atdetectionwavelength325nm.Result:ThehighestcontentofcaffeicacidwasdetectedinHerbaLycopifromHebeiamongtenhabitats,andthesamplecollectedinAugustfromBeijingshowedthehighestcontenta2mongfivesamplecollectedfromJunetoOctober.Conclusion:ThemethodissensitiveandaccuratewithgoodrepeatabilityandsuitableforthequalitycontrolofHerbaLycopi.[Keywords]HerbaLycopi;caffeicacid;HPLC;habitat;collectingtime[]198311120066ChinaJournalofChineseMateriaMedicaVol131,Issue11June,2006