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232Vol.23,No.220013ChineseJournalofPreventiveVeterinaryMedicineMar.2001,,(,310029),,,,;;;Q78;B1008-0589(2001)02-0157-03Mason1992,B(LT-B)(Norwalkvirus)10[17],[811]11.1,,,1.2,,;,1.3,,,,,,1.41.5,1.6,,,:(:30070570):2000-09-252,(),,,,[12,13],2.12.1.1:DNA,,,,,2.1.2:,,,,,,,,2.1.3:::,,,;,,:©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,DNA,,;,,,2.22.2.1:,,,DNA;(CaMV)(TMV)(CPMV)X(PVX)TMV1502.2.2::TiRiTi(Agrobacteriumtumefaciens),Ri(Agrobacteriumrhizogenis)TiRi,,TiDNA[1418]Ti:T-DNA(,)Vir(T-DNA)T-DNA1224kb,25bp,T-DNA[18,19],DNA,T-DNA,VirT-DNA35kb,T-DNA,Vir[20]VirTiT-DNATi,VirVIRD2,VIRD4VIRBTi,200800kb,DNA,TiTiTiT-DNADNA[21]:(,)TiE.Coli,Ti,,E.Coli2.3()T-DNA,VirT-DNA,Ti,T-DNA,,TiT-DNATiT-DNA(intermediatevector),TiTi(acceptorTiplasmid),(disarmedvector)TiTiT-DNAoncTi,T-DNAonc,,onc-T-DNApBR322pBR322DNApBR322DNA,onc-TiE.coli,Ti:[22],:(),Ti,T-DNA,,Vir,,[23],T-DNAVirTi:Ti(mi2ni-Tiplasmid)Ti(helperTiplasmid),T-DNAVir,T-DNATiT-DNAVirTi,(OriV),VirT-DNA,VirT-DNATi:Ti;,TiE.ColipRK2013E.ColiTipRK2013,8512001©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,TiE.Coli,TipRK2013,TiTi,;Ti,,,,3;,,,,,:DNAT-DNADNA,DNA,DNA,DNA,,;,,4,,,:(CaMV35S,);,RNA,,,,,,GUS,80%,13,,GUS,GUS[24][1]MasonHS,Man-kitLamD,ArntzenCJ.Proc.Natl.Acad.Sci.USA,1992,89:1175411749.[2]MoffatAS.Science,1995,268(5):658660.[3]HaqKI,MasonHS,ClementsJD.Science,1995,268:714716.[4]MasonHS,BallJM,ShiJJ,etal.Proc.Natl.Acad.Sci.,USA,1996,93:53355340.[5]SugiyamaY,HamamotoH,TakemotoS,etal.FEBSletters,1995,359:247250.[6]PortaC,SpallVE,LovelandJ,etal.Virology.1994,202:949955.[7]TurpenTH,ReinlSJ,CharoenvitY,etal.Bio/technology,1995,(13):5357.[8],,,.2000,8(1):8588.[9],,,.,1999(19):3944.[10],,,1998,18:5154.[11],,,.,1997,5:2125.[12]EambryuskiP.Ann.Rev.Genet.,1988,22:130.[13]HooykassPJJ,SchilproortRA.PlantMolec.Biol.,1992,19:1518.[14]AlbertsB,BrayD,LewisJ,etal.Molecularbiologyofthecell.3rded.NewYorkandlodon:GarlandPublishing,Inc.,1994.[15],.,1998,18(1):2226.[16]PaulJJ,Hooykass,RobA.PlantMolec.Biol.,1992,19:1538.[17],.,:.1990.[18]BartonK.A,Cell,1989,56:1033.[19]BaoY.J.Virology.,1996,70:63786383.[20]StachelSE,MessensE,VanMontaguM,etal.Nature,1986,318:624629.[21]FraleyRT.Biotechnology,1985,3:629635.[22],.,:,1988.[23]AnGBinary.MethodsEnzymol.1987,153:292305.[24]MoloneyMM.US.Patent1997,550554.9512,.©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.