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Preperationoftotalsapindus-saponinsforqualitycontrolH.Wu,C.Zhang,D.P.Xu,H.Y.Wang,W.R.YaoSchoolofFoodScienceandTechnology,JiangnanUniversity,Wuxi214122,ChinaSapindussaponinhaswonderfuldetergencywhichisnatural,environment-frendlyandnontoxic.Itcanbedevelopedintofoodindustrycleaningagent.However,thereisnopuresapindussaponincommerciallyavailable.WeproducedTotalsapindus-saponins,thepuritywerecalculatedtomorethan95.2%.TSSweremainlyoleanolicacidsaponinsandcanbeusedforqualitycontrolasstandard.Fig1ESI-ionchromatogramsofCSSIntroductionResultsTable1AnalysisofcomponentsofCSSNo.Time/minMFormula[M-H]-[M+Cl]-[M+Na]+Type14.431000C45H76O24999.511035.491023.55MukuroziosideⅠb25.061146C51H86O281145.581181.571169.63MukuroziosideⅡb35.411188Unknown1187.411223.461211.50Impurity45.791002C45H78O241001.521037.501025.56MukuroziosideⅠa56.221148C51H88O281147.601183.561171.63MukuroziosideⅡa66.65-7.601190C55H98O271189.771225.731213.71Unknown77.901044C33H58O151043.541079.551067.59Unknown88.49-9.841086Unknown1085.701121.681109.64Impurity1232C55H92O301231.621267.611255.68MukuroziosideA910.301206C58H94O261205.681241.671129.70Mukurozi-saponinY1074C53H86O221073.611109.591097.63Mukurozi-saponinX1011.201128C56H88O331127.711163.701151.69Unknown1113.761400Unknown1399.721436.701423.74Impurity1214.72882C46H74O16881.51918.51905.53SapindosideB1315.00750C41H66O12749.47785.45773.49SapindosideA1415.14924C48H76O17923.53959.52947.55Mukurozi-saponinE115.52924C48H76O17923.65959.63947.65SapindosideL1515.73966C50H78O18965.561001.54989.53Mukurozi-saponinG16.06966C50H78O18965.541001.52989.54SapindosideMMethodsFig2TheresultofseparationofMCIGELbyTLCdetection,thehemolytictestandESI-ionchromatograms(LeftRight)acbConclusionResultsResults2500002000015000100005000048121625000200001500010000300004812160123456789101112131415IoncurrentTime/minIoncurrentTime/minFig3TheUVgraphofOlenolicacidandsapindus-sapogenininvanilin-aceticacid-HClO4systemAbsWave(nm)1.51.00.0400.0600.0450.0500.0550.00.5549.0,0.9548.0,1.0sapindus-sapogeninOlenolicacidTable2Comparationofhydrolysisconditionsofthetotalsapindus-saponins(a-Heatunderreflux,b-100℃waterbath,c-Hightemperatureandpressure)ThepurityofTSS(TotalSapindusSaponin)werecalculatedtomorethan95.2%byacidhydrolysisundertheconditionofhightemperatureandpressure.(Table2)ItcanbefurtherconcludedthatTSSweremainlyoleanolicacidsaponinsandcanbeusedforqualitycontrolasstandard.TSSQuantitivetest:HighPerformanceLiquidChromatography/Massspectrum,vanillin–sulfuricacid.TSSQualitativetest:Hemolysistest.Totalsapindus-saponins(TSS)SapinduspericarpSolvantextractAB-8macroporousresinCrudesapindus-saponins(CSS)MCIGelHemolysistestof“c”provedthemosthemolytic.Thecomponents11~16wereanalysedbyHPLC/MS,identifiedasOleanolicacidsaponins.(Table1,Fig2)HydrolysisconditionsTriterpinoidsapogenin(mg)TSS(mg)TSSpurity(%)a37088888.8b33680680.6c39695295.2Oleanolicacidwaschosenasstandard.SapogeninofTSSafterhydrolysishadamaximumabsorptionat550nmwiththemethodofvanillin–sulfuricacid,whichwasthesameasOleanolicacid.(Fig3)abcAuthor:HengWu,MasterdegreeEmail:[e-mail]123456789101112131415