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PCRcDNA3123(2,230032)cDNAPCR,cDNAPCR;(300bp),PCRSorthern;PGEM2T,JM109,PCR,Sorthern115kbcDNA;GeneBankBlaste,,GeneBank(RatLiverRegeneration2relatedPro2tein1,RLRP21AF236054)PCRSortherncDNA;;PCR,[1];cDNA,PCRcDNA3(39670366)(00044202)1,1008503PCR11.1Wistar;cDNAClonetech;Promega;232P(dCTP);PCR()1.2RDA(,representationaldifferenceanalysis,RDA)EST,53DNAtools,BLAST10GenebankSlotBlotting,NorthernBlotting,(300500bp)[2]1.3PCR,cDNAT7/SP6cDNAPCR,PCR,PCR:P152AGTGCTATTGTGACCATCAAGATG23P252TTCTGGTCACTCCACATCAGCTCT23:T752ATTATGCTGAGTGATATCCC23SP652TAAATCCACTGTGATATCTT23T:10,(1/3),,30,,T36mon,T,,,,,,,,,1,++T:3,,1,,,,,T,,36mon,Mirizzi1/2,,Roux2YMirizzi,,,Mirizzi41CsendesA,CarlosJ,BurdilesPetal.Mirizzisyndromeandchole2cystobiliaryfistula:Aunifyingclassification.ByJSurg,1989;76(11):11392,..1,:,2000;1:10693McsherryCK,FerstenbergH,VirshupM.TheMirizzisyndrome:SuggestedclassificationandSurgicaltherapy.SurgGastroenterol,1982;1:2194,,etal.Mirizzi.,2000;20(12):727885AnhuiMedicalandPharmaceuticalJournal2003Feb;7(1):1.4Promega:(cDNA)150ng,15l,1005min,,Promega:5XLabelingbuffer5lBSA1ldNTPmix1l32p2dCTP215lTKRKenown015l(25)1h,SephdexG250,-201.5SorthernPCR,[1],1h,20minNC(15h),NC802h,BioDev651h,,65NC2SSC015SDS2,015SSC015SDS502,20min,,-701.6Sorthern,,,BioDevDNA(BioDevDNA),PGEM2T,JM109,1.7,Promega(),PE,BLASTGeneBank(expressedsequencetags,EST)22.1PCR,cDNA,cDNA,PCRPCR110%,P1+T7PCR014kb;P2+SP6PCR115016kb;P2+T7,P1+SP6PCR2.2SorthernPCR110%,Sorthern,:P2+SP6PCR,1PCRM:DL2000Maker,A:P2+T7,B:P2+SP6,C:P1+SP6,D:P1+T7,E:P1+P22Sorthern115kb016kb2.3PGEM2T,,,PET7/SP6,BLASTGeneBank,,21(RLRP21)GeneBank:AF2360543[3][4]RDA(Microarray)[57]EST,,(ORF),cDNAEST52RACE,ClonetechPositivecDNAClonecaptureKitcDNA;;,PCRSorthern,cDNAcDNA,,,,PCR,Taq(AdvantageDNAPloymerase)SorthernCDNAPCRPCR95AnhuiMedicalandPharmaceuticalJournal2003Feb;7(1)1J.,E.F.,T...,:,1999:60752Xuwangxiang,Wangsiying,Wanggeetal.IdentificationandCharacterizationofdifferentillyExpressedGenesintheearlyResponsePhaseduringtheLiverRegeneration.BiochemandBiophysResComm,2000;278(2):318253TapperoG,FarinaM,NegroFetal.Intrahepaticexpressionofc2fos,c2mybandc2myconcogenes:correlationwithvirus2inducedchronicliverdiseaseandresponsetointerferon.ItalJGastroenterolHepatol,1997;29(2):148544YeeKW,OFarrellAM,SmolichBDetal.SU5416andSU5614inhibitkinaseactivityofwild2typeandmutantFLT3receptortyrosinekinase.Blood,2002;15(8):294195,,etal..,2000;35(6):42246SchenaM,RenuA.Microarrys:biotechnologydiscoreryplatformforfunctionalgenomics.Tib,1998:16227UrbanelliL,RonchiniC,FontanaLetal.TargetedGeneTransduc2tionofMammalianCellsExpressingtheHER2/neuReceptorbyFila2mentousPhage.JMolBiol,2001;313(5):9657606AnhuiMedicalandPharmaceuticalJournal2003Feb;7(1)