pGL3-Basic-Vector质粒图谱及其说明

TechnicalManualpGL3LuciferaseReporterVectorsINSTRUCTIONSFORUSEOFPRODUCTSE1741,E1751,E1761ANDE1771.PRINTEDINUSA.Revised12/08Part#TM033AF9TM0331208TM033tm033.1208.qxp12/31/20089:15AMPageaPromegaCorporation·2800WoodsHollowRoad·Madison,WI53711-5399USATollFreeinUSA800-356-9526·Phone[phone]·Fax[phone]·::[e-mail]12/31/20089:15AMPage11.DescriptionThepGL3LuciferaseReporterVectors(a–c)provideabasisforthequantitativeanalysisoffactorsthatpotentiallyregulatemammaliangeneexpression.Thesefactorsmaybecis-acting,suchaspromotersandenhancers,ortrans-acting,suchasvariousDNA-bindingfactors.ThebackboneofthepGL3LuciferaseReporterVectorsisdesignedforincreasedexpression,andcontainsamodifiedcodingregionforfirefly(Photinuspyralis)luciferasethathasbeenoptimizedformonitoringtranscriptionalactivityintransfectedeukaryoticcells.Theassayofthisgeneticreporterisrapid,sensitiveandquantitative.Inaddition,theseLuciferaseReporterVectorscontainnumerousfeaturesaidinginthestructuralcharacterizationoftheputativeregulatorysequencesunderinvestigation.2.ProductComponentsandStorageConditionsProductSizeCat.#pGL3-ControlVector20μgE1741pGL3-BasicVector20μgE1751pGL3-PromoterVector20μgE1761pGL3-EnhancerVector20μgE1771Informationonrelatedproducts,includingtheLuciferaseAssaySystem,isprovidedinSections4–7and8.K.StorageConditions:StorethepGL3LuciferaseReporterVectorsat–20°C.3.pGL3VectorMapsandSequenceReferencePointsThelistingsofrestrictionsitesforthepGL3LuciferaseReporterVectorsareprovidedinSectionVIII.G–J.Note:ThespecifictranscriptionalcharacteristicsofthepGL3Vectorswillvaryfordifferentcelltypes.ThismaybeparticularlytrueforCOScells,whichcontaintheSV40largeTantigen.TheSV40largeTantigenpromotesreplicationfromtheSV40origin,whichisfoundinthepromoterofthepGL3-PromoterandpGL3-ControlVectors.ThecombinationoflargeTantigenandSV40originwillresultinahighercopynumberofthesevectorsinC