鸡N-乙酰-β-D-氨基葡萄糖苷酶ELISA试剂盒说明书

鸡N-乙酰-β-D-氨基葡萄糖苷酶ELISA试剂盒说明书鸡N-乙酰-β-D-氨基葡萄糖苷酶ELISA试剂盒说明书保存条件：2-8℃低温保存供应商：上海樊克生物有限公司保质期：6个月，所有试剂盒均提供最新批次。ELISA试剂盒规格：(1)规格：96T可以测90个样，5个标准孔，1个空白孔(2)规格：48T可以测42个样，5个标准孔，1个空白孔【ELISA试剂盒种属】人、大鼠、小鼠、豚鼠、兔子、猪犬、牛羊、鸡鸭、植物ELISA试剂盒等。【试剂盒检测目的】用于测定血清，血浆及相关液体等样本。例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。【标本】血清、血浆、细胞上清液、尿液、体液、灌洗液、脑脊髓、心房水、胸房水、组织等。【用途】科研实验等领域科学研究，不得用于临床诊断。elisa试剂盒价格，elisa试剂盒说明书，elisa检测试剂盒计算：Tothestandardconcentrationastheabscissa,ODvalueastheordinate,drawthestandardcurveoncoordinatepaper,accordingtotheODvalueofsamplesfromthestandardcurvefoundcorrespondingconcentration;multipliedbythedilutionmultiple;orwiththestandardconcentrationandtheODvaluecalculatedstraightlineregressionequationofthestandardcurve,thesampleODvalueintheequation,calculatethesampleconcentration,multipliedbythedilutionfactoristheactualconcentrationofthesample.鸡N-乙酰-β-D-氨基葡萄糖苷酶ELISA试剂盒说明书Experimentalprinciple:Determinationofgonadotropinlevelsinmicechorionicspecimensofthekitusingdoubleantibodysandwichmethod.Usingpurifiedmousechorionicpromotegonadalhormoneantibodycoatedmicrotiterplate,madeofsolidphaseantibody,tocoatedmicroporousmonoclonalantibodiesfollowedbyaddingcollagenase,combinedwithHRPlabeledcollagenaseantibody,theformationofantibody,antigenandenzymelabeledantibodycomplexes,afterthoroughwashing,TMBsubstrateaddedcolor.TMBunderthecatalysisoftheHRPenzymeintoblue,andundertheactionofacidintothefinalyellow.Collagenasewaspositivelyrelatedtothedepthofcolorandsamples.Enzymestandardinstrumentin450nmwavelengthwasusedtomeasuretheabsorbance(OD),throughthestandardcurvecalculationofasmallsampleinchorionicgonadotropinconcentration.鸡N-乙酰-β-D-氨基葡萄糖苷酶ELISA试剂盒说明书Operationsteps10孔，在第一、第二孔中分别加标准品100μl，然后在第一、第二孔中加标准品稀释液50μl，混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第Tenthnineholeswereaddedtothestandarddilutionliquid50L,aftermixingfromtenthholesinthe50totakeninthMuLabandoned.(dilutedsampleamountforeachholeconcentrationof50L,respectively1800ng/L,1200ng/L,600ng/L,300ng/L,-addsample:arerespectivelyprovidedwithablankhole(blankcontrolwellswithoutsampleandELISA,therestoftheeachstepoperationissame),sampleholetobemeasured.IntheenzymestandardcoatedplatetobetestedonasampleholeZhongxiansampledilutionof40gl,andthentobemeasuredisadded10mulofsample(samplefinaldilutiondegreesfor5times).Thesampleisaddedtothebottomoftheenzymelabeledplatehole,asfaraspossiblewithouttouchingthewallofthehole.Havingincubationwithclosureplatemembranesealingplaterear37DEGCincubationfor30minutes.Collaboratedwithliquid:30(20times48T)timesconcentratedwashingliquidwithdistilledwater30times(20times48T)dilution.-washing:becarefultornoffthesealplatemembrane,discardliquid,drying,washingliquidtofilleachhole,standingfor30secondsafterthediscard,repeat5times,patdry.Falls:enzymeperholeaddingenzymereagent50L,excepttheblankhole.HavingIncubation:operationwith3.-washing:operationwith5.-Color:eachholetojointhechromogenicagentA50MuL,addingchromogenicagentB50MuL,gentlyshakemixand37DEGCtoavoidlightcolorfor15minutes.Havingterminated:perholeaddingstopsolution50Lterminationreaction(thebluecolorturnyellow).Havingdetermined:theblankairconditioningzeroabsorbanceat450nminordertomeasurethehole(OD).Shouldbemeasured15minutesafterthestopsolutionandwithin.鸡N-乙酰-β-D-氨基葡萄糖苷酶ELISA试剂盒说明书1standardproducts:seriesofdilutionofthestandardproductshouldbepreparedintheexperiment,cannotbestored.Beforedilution,thestandardproductismixedevenly.Dilutionratioiscarriedoutinthetable:40umol/L(standard6)theoriginalconcentrationoftheconcentrationwithoutdilutiondirectlyintothe50ul.20umol/L(standard5)100uloftheoriginaltimesthestandardtojointhe100ulstandarddilution10umol/L(standard4)100ul5standardtojointhe100ulstandarddilution5umol/L(standard3)100ul4standardtojointhe100ulstandarddilution2.5umol/L(standard2)100ul3standardtojointhe100ulstandarddilution1.25umol/L(standard1)100ul2standardtojointhe100ulstandarddilution0umol/L(blankcontrol)theoriginalconcentrationisnotdiluteddirectlyintothe50ul.2washingbuffersolution(50*)dilution:distilledwater50timesdilution.上海樊克生物有限公司专业的提供elisa试剂盒，人elisa试剂盒，猪elisa试剂盒，大鼠elisa试剂盒，小鼠elisa试剂盒，羊elisa试剂盒，植物elisa试剂盒等等，更多优惠，来电咨询！谢谢！注：本产品只用于科研实验不用于临床诊断。