水通道蛋白研究进展

:1004-5422(2010)02-0104-03蒲春霞(成都大学医护学院,四川成都　610017)　:水通道蛋白广泛存在于生物体中的各组织部位,影响着生物机体水代谢的过程,随着分子生物学技术的进步,对水通道蛋白的基础研究已经比较深入和成熟.对水通道蛋白的研究情况进行概要综述,目的在于利用水通道蛋白研究的基础成果,阐释临床水代谢障碍类疾病的发病机理及提供可能的解决思路.:水通道蛋白;水代谢;研究进展:R329　　　　　　　　　　:A0　　(aquaporin,AQPs),Agre1988,28(CHIP),1991,cDNA,CHIP28AQP1.,9(AQP2～AQP10).,(majorintrinsicprotein,MIP),MIP,AQP0.AQPs[1,2].1　2,——(Asn-Pro-Ala,NPA),180°.65(A、B、C、D、E),:B、D、,A、C、E.B、E,E、B;AN-;ENPA[3].AQP1.AQP14.4,,318A.[3,4].、.,:AQP0,AQP1、、,AQP2,AQP3、、;AQP4、、、、,AQP5,、、,AQP6,AQP7,AQP8,,AQP9,AQP10.,AQP1AQP5[5,6].2　AQPsAQPs2:,AQP0、AQP1、AQP2、AQP4、AQP5、AQP6、AQP8;,,,AQP3、AQP7、AQP9、AQP10.AQPs,:2.1　AQP1～AQP4AQP.AQP1,,AQP1AQP3.AQP4.:2009-11-25.:(1974—),,,,.AQP1,.AQP110,AQP1.AQP2、AQP3AQP4.AQP3AQP4.AQP3,AQP4.,,AQP3AQP4.AQP4..,AQP2[7-9,15].2.2　,AQP1～AQP5、AQP8、AQP9.AQP1,,.AQP4、、、、、.TsukaguchiAQP9mRNA,Badaut2001AQP9,,AQP4AQP9.AQP9AQP4.Tomas(LPS)AQP4AQP4mRNA,、AQP4[9-10].Nicchia,AQP4[11,12,14,20].AQP4,AQP9,AQP8.[12,13].2.3　,.,AQP1,AQP4,AQP5.AQP5,,AQP1AQP4[15],,AQP5.AQP4,AQP4.AQP1,、、[11,12].2.4　3AQP,AQP10.3mm,AQP4,AQP5.,AQP3,,[6,17].2.5　AQP1,AQP1215,AQP1.AQP4,AQP4.AQP1.AQP1.,AQP1,AQP5.AQP3,AQP3[13,14].2.6　,AQP4,.AQP0[15,16].3　AQPs3:AQP.AQP1、AQP2、AQP4、AQP5A(proteinkinaseA,PKA)C(PKC),.,AQP、.AQP1、AQP2、AQP4、AQP5.3pH.AQP,AQP1、AQP2、AQP5、AQP8,,[4,9,10,19,20].4　　AQPs10,AQPs.AQPs·105·第2期　　　　　　　　　　　　　蒲春霞:水通道蛋白研究进展,,,.:[1]PrestonGM,CarrollTP,GugginoWB,etal.AppearanceofwaterchannelsinXenopusoocytesexpressingredcellCHIP28protein[J].Science,1992,256(5055):385-387.[2]BengaG.Birthofwaterchannelproteins———theaquaporins[J].CellBiolInt,2003,27(9):701-709.[3]刘树荣,张少斌.水通道蛋白结构与功能研究进展[J].现代预防医学,2007,34(12):2260-2261.[4]NemethCKL,KalmanK,HallJE.MolecularbasisofPHandCa2+regulationofaquaporinwaterpermeability[J].TheJournalofGeneralPhysiology,2004,123(5):573-580.[5]马　磊,何　华.兴奋或则抑制汗液分泌对小鼠汗腺水通道蛋白免疫定位的影响[J].中国组织化学与细胞化学杂志,2008,17(6);555-556.[6]TowneJE,KraneCM,BachurskiCJ,etal.Tumornecrosisfac-toralphainhibitsaquaporin-5expressioninmouselungepithelialcells[J].JBiolChem,2001,276(22):18657-18664.[7]范亚平,蒋季杰.水通道蛋白与肾脏[J].国外医学泌尿系统分册,2001,21(5):215-219.[8]GradiloneSA,GarciaF,HuebertRC,etal.Glucagoninducestheplasmamembraneinsertionoffunctionalaquaporin-8waterchannelsinisolatedrathepatocytes[J].Hepotology,2003,37(6):1435-1441.[9]BengaG,PopescuO,BorzaV,etal.Waterpermeabilityinhumanerythrocytes:Identificationofmembraneproteinsinvolvedinwa-tertransport[J].EurJCellBiol,1986,41(2):252-262.[10]VerkmanAS.Applicationsofaquaporininhibitors[J].DrugNewsPerspect,2003,14(7):412-420.[11]YasuiM,HazamaA,KwonTH,etal.Rapidgatingandanion-permeabilityofanintracellularaquaporin[J].Nature,1999,402(67):184-187.[12]YangB,GillespieA,CarlsonEJ,etal.Neonatalmortalityinanaquaporin-2knock-inmousemodelofrecessivenephrogenicdiabetesinsipidus[J].JBiolChem,2001,276(4):2775-2779.[13]VerkmanAS,YangB,SongY,etal.Roleofwaterchannelsinfluidtransportstudiedbyphenotypeanalysisofaquaporinknock-outmice[J].ExpPhysiol,2000,85(1):233-241.[14]KageyamaY,IshibashiK,HayashiT,etal.Expressionofaqua-porins-7and-8inthedevelopingrattestis[J].Andrologia,2001,33(3):165-169.[15]MaT,YangB,GillespieA,etal.Severelyimpairedurinarycon-centratingabilityintransgenicmicelackingaquaporin-1waterchannels[J].JBiolChem,1998,273(8):4296-4299.[16]MaT,YangB,GillespieA,etal.Generationandphenotypeofatransgenicknock-outmouselackingthemercurial-insensitivewaterchannelaquaporin-4[J].JClinInvest,1997,100(5):957-962.[17]VerkmanAS,LiJ,MaT,etal.Roleofaquaporinwaterchan-nelsinkidneyfunctionstudiedusingtransgenicmice[J].ClinExpNephrol,2001,5(1):75-84.[18]VerkmanAS.Aquaporinwaterchannelsandendothelialcell-function[J].JAnat,2002,200(6):617-627.[19]HaraM,VerkmanAS.Glycerolreplacementcorrectsdefectiveskinhydration,elasticity,andbarrierfunctioninaquaporin-3-deficientmice[J].ProcNatlAcadSciUSA,2003,100(12):7360-7365.[20]WardDT,HammondTG,HarrisHW.Modulationofvaso-pressin-elicitedwatertransportbytraffickingofaquaporin-2-con-tainingvesicles[J].AnnuRevPhysiol,1999,61(1):683-697.ResearchProgressonAquaporinPUChunxia(SchoolofMedicineandNursing,ChengduUniversity,Chengdu610106,China)Abstract:Aquaporinswidelyexistineverytissuecompartmentoforganismwhichinfluencestheprocessofwatermetabolismoforganism.Withtheadvancementofbiologictechnologies,basicresearchesonaquaporinhavebeenfurtherdeepenedandbecomemature.Recentresearchesonaquaporinweresummarizedinordertoutilizetheseresearchachievementstoexplainpathogenesisofdiseaseswhendisturbanceofwaterme-tabolismappearandtoproposeresolvingways.Keywords:aquaporins;watermetabolism;researchprogress·106·　　　　　　　　　　　　　　　成都大学学报(自然科学版)　　　　　　　　　　　　第29卷