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413()Vol.41No.320025JournalofXiamenUniversity(NaturalScience)May2002:043820479(2002)0320330204:2001206228:(F0100008):(1968-),,.1,1,1,1,WilsonDavidB2(1.,361005;2.Dept.ofMolecularBiologyandGenetics,CornellUniv.,USA):.2.58mg/L,,pH9.6.,,,30%.pH,pH.EDTA.:;;;:X131.2:A,[1].,[2].CornellWilson,[3].,,.,.11.1WilsonE.coliJM109,DNA,,.,,.,(Amp)(Kan).1.250mg/L30mg/LLB(Luriabroth),37.LB,OD6000.10.3,37OD6000.50.72D2(IPTG)1.0mmol/L.4h,4.,,371h,.,70%.,0.2m,.[4],20%,.1.3,600nm,Cornell2.22.1Syracuse,.NaClCl2NaOH.,,NaCl.12.,2.58mg/L,,.,().NaOH,pH,9.6.,,.1Tab.1Componentsofelectrolytewastewater/mgL-1/mgL-1Ca3.53Mg21.75Fe0.16Mo0.14Hg2.58Na1550K40.58Sr0.36Li0.46Zn0.112.20.35g/L.,2.58mg/L.,5min,5min(1).1,Hg2+:2.58mg/L,,Hg2+:2.58mg/LFig.1Comparisonofbioaccumulationrate2EDTANaCl4mg/LHg2+,4mg/LHg2++0.05mmol/LEDTA,4mg/LHg2++0.1mmol/LNaClFig.2EffectofEDTAandNaClonbioaccumulationrate1,,.,EDTANaCl.2EDTANaCl.,,.1333:2.3(2.58mg/L).pH,pH.3(CD),(AB,ApH9.67.6)30%..[5],,,.3A:,pH7.6,B:,pH9.6,C:,pH7.6,D:,pH7.6Fig.3Comparisonofequilibriumbioaccumulation3pH.pH,pH.,.,(D)(C),,.2.4.(biomass)()..,,5mg/L.,,,,..4mg/L,1.56mg/g,10.2mg/g,7.3.,30%.pH,.:[1]SpryDJ,WernerJG.Metalbioavailabilityandtoxicitytofishinlow2alkalinitylakes:acriticalreview[J].Envi2ron.Pollut.,1991,71:243-304.[2]LiuRX,TangHX,ZhangBW.RemovalofCu,Zn,CdandHgfromwastewaterbypoly(acy2laminophosphonic)2typechelationfiber[J].Chemo2sphere,1999,38(13):3169-3179.[3]ChenSL,WilsonDB.GeneticenigneeringofbacteriaandtheirpotentialforHgbioremediation[J].Biodegrada2tion,1997,8:97-103.[4]JenneEA,AvotinsP.Thetimestabilityofdissolvedmercuryinwatersamples[J].J.Environ.Qual.,1975,4:427-431.[5]KuyucakN,VoleskyB.Accumulationofgoldbyalgalbiosorbent[J].Biorecovery,1989b,1:189-204.233()2002BioaccumulationofMercurybyGeneticallyEngineeredEscherichiacolifromRealElectrolyticWastewaterDENGXu1,LIQing2biao1,SUNDao2hua1,HUANGYi2li1,WILSONDavid2B2(1.Dept.ofChem.Eng.,XiamenUniv.,Xiamen361005,China;2.Dept.ofMolecularBiologyandGenetics,CornellUniv.,Ithaca,NY14850,USA)Abstract:BioaccumulationperformanceofmercuryfromrealelectrolytewastewaterbygeneticallyengineeredE.coliJM109,whichexpressedamercurialiontransportsystemandmetallothioneinwasinvestigated.Theseweremorethan10componentsexistinginthewastewaterbesides2.58mg/Lmercury,andthepHofthewastewaterwas9.6.Theco2ionsdidntdecreasebutsomehowpromotedmercuryuptakerateofinducedcells,whilea30%lossoftheamountofmercurybioaccumulationwasobtainedincomparisonwiththatfromlaborato2rysurrogatesolutiononlycontainingmercury.ThevaryingofpHinwastewateronlyslightlydroppedmercuryaccumulation,suggestinginducedcellshadapropertyofpHinsensitivity.Keywords:geneticallyengineeredbacteria;bioaccumulation;wastewatertreatment;mercurialion3333: