脊髓灰质炎病毒sabin株工作种子批基因鉴别

Sabin廖国阳孙明波余芬沈伟赵玮蒋燕军姜述德Sabin!∀Sabin#Pfizer3RTPCR,SDSPAGE!∀Sabin#Pfizer3977153bp,RTPCRSabin;;RTPCR!∀#,RNA,,,Sabin,Sabin1WHOType1SabinseedSO+1,WHOType2SabinseedSO+1WHOType3457PfizerRSO1Vero:!SabinSO+2,UPV9102;∀SabinSO+2,UPV9103#;PfizerRSO2,UVP95012RNAOneStepRTPCR,Qiagen3!F:5∃TCCACTGGCTTCAGTGTT3∃,!R:5∃AGGTCAGATGCTTGAAAGC3∃;∀F:5∃CGGCTTTGTGTCAGGC3∃,∀R:5∃CCGTTGAAGGGATTACTAAA3∃;#F:5∃AGTATCAGGTAAGCTATCC3∃,#R:5∃AGGGCGCCCTAACTTTG3∃()4[1]10,:(2000XY05).:IPV(650118).:,Te:l08718334551,Emai:[e-mail]2(),(36%1)&,7d5!∀#,,(36%1)&1h,Hep2,(36%1)&,7d,,6RNAQiagenQIAampRNARNAQiagenOneStepRTPCR:H2O235,l5∋buffer10,ldNTP2,lEnzyme2,lRNasin05,lRNA10,l1,l1lRT:50&30min,95&15minPCR:95&45s,55&45s,72&45s,30,72&10min20%10%,(1g/ml),T[()],DNA1Sabin,Sabin!862LogCCID50/m,l∀812LogCCID50/m,l#787LogCCID50/mlSabin,,,3:UPV9102!,UPV9103∀,UVP9501#,1(376(20067194ChinJBiologicalsJuly2006,Vo1.19No.41Sabin!∀#UPV9102-++-++-UPV9103+-+-++-UVP9501++--++-2RNA(IPV),!∀#SabinRTPCR,DNA(OPV),!SabinRTPCR,97bp;∀SabinRTPCR,71bp;#PfizerRTPCR,53bp,11:M199;2:OPV#;3:OPV∀;4:OPV!;5:#PfizerRSO2;6:∀SabinSO+2;7:!SabinSO+2;8:25bpDNAmarker;9:IPV.1SabinRTPCR3,!Sabin:TGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACGATTCCACTGGCTTCAGTGTTTGGGAGAGCGTCTCTAGACGTTGCCGCCCCCACCGTTTCACGGACTGTGTTGTCAATCATGCTTTCAAGCATCTGACCTAATCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGA!Sabin!Sabin,RTPCR!Sabin2385~2481(97),!∀Sabin:TTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACGATTCGGCTTTGTGTCAGGCAGGCTATTGGTGGAAGTCGGGGGAACCAATGCATTTTTAGTAATCCCTTCAACGGAATCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGA,∀Sabin2507~2577(71),∀#Pfizer:TTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACGATTAGGGCGCCCTAACTTTGTCACTCCCGAAGCAACAGGATAGCTTACCTGATACTAATCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGA#Sabin2508~2560(53),#,,OPV(VAPP)(VDPV)[2]IPV(380)(377(20067194ChinJBiologicalsJuly2006,Vo1.19No.4,,[2]Tohru[3],BCG,BCGMER(BCG),BCGCWS(BCG)BCGMDP(BCG),BCGPSN(),T,IFNIL2[4](IL2IL4IL6IFN)rBCG,[5],,CMIDTH[6],,,CMIDTH,PPD,PPDPPD,15mm,,[1]..:,2001.95~99.[2],..,1998,11(3):183~186.[3]TohruT,HiroshiY,ShizuoSY,eta.lAntitumoractivityofDNAfractionfrommycobacteriumbovisBCG1,Isolation,physicochemicalcharacterrizationangantitumoractivity.JNailCancerInst,1984,72(4):18.[4],,,..,1999,12(4):208~209,224.[5],..,2002,25(2):55~59.[6],..:,2004.787.(:20050627)(377)VAPPVDPV,,BSL4,[3,4]Sabin,,,WHOSabinIPV[5,6],SabinBehringwerkeAG.MarburgLahnInstitutMerieux,,,Sabin,RTPCRSabinWHO,!∀#Sabin,,3Sabin,Sabin,[1]Requirementsforpoliomyelitisvaccine(Inactivated).WHOTRS910Annex2,Revised2000.[2]LaassriM,LottenbachK,BelsheR,eta.lEffectofdifferentvaccinationschedulesonexcretionoforalpoliovirusvaccinestrains.JInfectDis,2005,192(12):20922098.[3]KerstenG,HazendonkT,BeureryC.AntigenicandimmunogenicpropertiesofinactivatedpoliovaccinemadefromSabinstrains.Vaccine,1999,17(1516):20592066.[4]DoiY,AbeS,YamamotoH,eta.lProgresswithinactivatedpoliovirusvaccinesderivedfromtheSabinstrains.DevBiolBaselKarger,2002,105:163169.[5]GuidelinesforthesafeproductionandqualitycontrolofIPVmanufacturedfromwildpoliovirusbiosafetyLevel3/Polio.WHOTRSNO926.2004.[6]WHOglobalactionplanforlaboratorycontainmentofwildpolioviruses(Secondedition).Uirusu,2005,55(1):161178.(:20060303)(380(20067194ChinJBiologicalsJuly2006,Vo1.19No.4