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PlantPhysiologyandAgriculture20002122192001212203(,100037)GeneticEngineeringandFoodIndustryPEILing2Peng,HUANGQin2Ni(DepartmentofBiology,CapitalNormalUniversity,Beijing100037),,,(geneticengineering)DNA,,,,161.1,,:[1];,,[1];,,[1]10000,[2],,CalgeneSheeg[3]35SCaMV(cauliflowermosaicvirus)PGDNA,,,,PGRNA,70%90%,,,,,1.220,:,,,,:NorternDREB1DREB2,DREB1,DREB2[4],[4],[4]1[5],,(2)[6]1.3Lancashine[7],,,,,Lancashine[8],,,,,()[9],,Feller[10](moraxella)TA144692383,20026,1[5]P5cs252P5cs252CodAMltd212TPS1262SacBHVA1LeaMn2SOD2[6]KurstakiCryIA(b)(Ostrinianubilalis)300,,,,BtCryIA(b)3646aaTi35S:8.7%;22.3%:4.1%;17.8%Actinpr/CpTIBtCryA(Ceptinotarasadecemlincatal):,,;:,3(Lactobacillus),(Lactococcus);;(Saccharomycescerevisiae),,1.4,,[11],Henahan[12](Bacil2lus),EcoRIDNA,,,710,[13](Pseu2domonaspseudoalcaloenes)HB101,PHZ1408,101.5,,Golman[14],,,,,1.6,Gol2man[14],,,,,Genzyme[15]3,,21985,(USDA)[16]2584,27.6%26.7%24.3%10.4%3.8%7.2%()792383,20026,,,,,??,,,,,[17]:(1),;(2);(3);(4),2.1(OECD)1993,,,[18],Nebraska[17]2SDNAnpt2EPSPS[19],WHO(1991)FAD(1994)[20]2.21996863,GFMCryIA,,,Bt,Bt[21],,1996726,20011,..,2001,36(2):672,.10kD.,2000,45(12):131313173SheeyRE.ReductionofpolyglaoturonaseactivityintomototruitbyantisenseRNA.ProcNatlAcadSciUSA,1988,85:880588094Pilon2SmitsEAH,EbskampMJM,PaulMJetal.Improvedper2formanceoftransgenicfuctan2accumulatingtobaccounderdroughtstress.PlantPhysiol,1995,107:1251305,..,1999,16(5):5555606..,1993,13(5):187LancashineWE.SuperttenuatingBrewihgYeast.EuroBioCongress,1989.48988LancashingWE.Moderngeneticsandbrewingtechnology.Brew2er,1986,72:3459,..,1999,11(2):14315010FellerG.Nucleotidcsequenceofthelipasegenelip2fromthean2turcticpychrotrophMoraxellaTA144andSite2Specificmutage2nesesoftheconseruedserineandhistidine.DNACellBiol,1991,10:38138411WoodsJ.Thegeneticengineeringofmicrobialsoventprodution.TrendsBiotechnol,1995,13(7):25926412HenahanJ.Geneticialengeeringinfuture.JBiotechnol,1984,(2):21221513,..,1999,19(4):283214GolmanA.Productionofproteintransgenticlivestock:problem,solutionandsuccess.AmercianJClinNutr,1996,63:63964515ElbertKM,SelgrathJP.Transgenticproductionofavariantofhumantissuretypeplasminogenactivatorongoatmilk:generationoftransgenticgoatsandanalysisofexpression.Biotechnol,1991,91:83583816..,1999,35(6):48649017..,1997,17(6):374218..,1997,(1):4919..,1997,30(2):11520..,2001,36(2):181921,..:,2000.61892383,20026