如何回复审稿人意见(Response-to-Reviews)

DearEditor,Wehavestudiedthevaluablecommentsfromyou,theassistanteditorandreviewerscarefully,andtriedourbesttorevisethemanuscript.Thepointtopointrespondstothereviewer’scommentsarelistedasfollowing:Respondstothereviewer’scomments:Reviewer1Comment1:inpage3,line40,wefedrats...changedtoratswerefedwith...Response:Accordingtothereviewer’scomment,wehavecorrectedthesentence.Furthermore,wehavehadthemanuscriptpolishedwithaprofessionalassistanceinwriting.Comment2:page25.Thestyleofreference40isnotright(usinginitialsforthefirstnames).Sincethispaperhasbeenpublished,thevolumeandpageNosshouldbeprovided.Response:Thankyouforyourcarefulwork.Wehaveaddedthevolumeandpagenumbersforreference40.Reviewer2Comment:Iwouldliketothanktheauthorsfortheireffortsinaddressingthecriticismswithadditionalexperiments.Theonecriticismthattheydidnotaddresswasrelatingtoenergyexpenditureasthereasonthattheanimalsonthelowcalciumdietgainedmoreweight.WhileIunderstandthatperformingthisexperimentwillnotaffecttheconclusionofthismanuscript,IdobelievethatthispointcouldbediscussedintheDiscussionsection.Response:Thankyouforyourvaluableadvice.Basedonthepreviousrevision,wefurtheraddresstherelationshipbetweenlowcalciumdietandenergyexpenditureinthesectionofdiscussionaccordingtoyourthoughtfulcomments.Reviewer3Comment1:Inthetextyouoftenwrite:“Aspreviouslydescribed”.Unlessthatpaperisfromyourlaboroneofthemethodpaperco-authorsisonthepresentMSthisisnotquitepropersincethestatementinfersmethoddevelopmentfromyourlab.Therearenumerousinstanceslikethatinthemethodssection;theseshouldallbechanged“accordingtothosedescribedby…..”Response:Wearesorryforthislanguagemistake.Wehavecarefullycorrectedthisphrasethroughoutthemanuscriptaccordingtoyourcomment.Comment2:Therearestillsomewording,sentencestructureandgrammaticalissueseveninthisbasicallywellputtogetherMS.Forexample,whileauthorsmayhavebeenexcitedaboutthedatayoucannotstartasentencewith“Excitedly”inline418or“Whatever”inline395.Response:Thankyouverymuchtopointoutthesentencestructureandgrammaticalissuesinourmanuscript.Accordingtothecommentsfromyouandtheeditors,wepolishedthemanuscriptwithaprofessionalassistanceinwriting,conscientiously.Comment3:Inmyviewabigomissioninthisworkisignoringtheanabolicsideoflipidmetabolismaswellasthermogenesisissues.ForexampleallanimalsconsumedthesameamountoffeedbutwehadextrafatstorageinthelowCadietgroups.Sowheredidtheextraenergygo?Zemeletal(citation34)insimilarworkindicatethatincreasedthermogenesisonthehighCadietexplainsthedissipationofdietaryenergy.FurthereventhoughZemeletal(#34)indicatedlipogenesiswasenhancedinthelowCadietsthatwasin2000andyoushouldhavemonitoredexpressionofFASandUCPeitherasmRNAabundanceoractualFAS/UCPchangesviaproteomicsorblottingtechniques.InanycasethesecontrolsaremissinghereandnotemphasizedintheMS.CasualreadingofthispaperwouldleadtotheconclusionthatthedietaryCaeffectonfatdepositionisstrictlyafunctionofincreasedordecreasedlipolysis.Whilelipolysisappearstobeamajorplayer,lipogenesisandthermogenesiscannotbeignoredforcompleteness.InFig8youalsoshowadeclineincAMPforthelowCadiet.WellbetaagonistsorcAMPenhancersregulatetranscriptionofadiposeandliverFAS(inrats(JBiolChem271:2307,1996)andrecentlywithlargeanimalmodels(HausmanetalJAnimalScience87:1218,2009andHalseyetalJAnimalScience89:1011,2011).InadditioncAMPlevelscouldhavebeenmonitored.IreallydonotlikethelastsentenceintheAbstractline47-50whereyoustatethat“lowcalciumdiet-inducedincreaseinfatmasswasduetoenhancedlipogenesismediatedbyanupregulatedCaSRsignalingpathway”Yourresultshereshownosuchthing,thisisacompletelyfalsestatementbasedondataherein.Correct.YoushowthathighCadietsenhancelipolysisandlowCadietsareantilipolytic.Youdidnotmonitorlipidanabolismhereatall.Seealsoline255-257andlines333-335ofyourMS.Response:Thankyouforyourvaluableandthoughtfulcomments.Asyousuggestedthattheanabolicsideoflipidmetabolismaswellasthermogenesisissuesshouldbemonitored.Wereallyagreewithyourviewpoints.Inthepresentstudy,wedidfindthatlowcalciumdietincreasedthemRNAleveloffattyacidsynthase(FAS)inwhiteadiposetissue.Furthermore,theFASmRNAlevelwerealsoincreasedinadipocytesaftertreatmentwith1,25-(OH)2D3inin-vitroexperiments.However,theincreasedFASmRNAlevelswerenotaffectedbypreventingeitherthenuclearvitaminDreceptor(nVDR)orcalcium-sensingreceptor(CaSR),suggestingthatFASmightnotbeinvolvedintheCaSRpathway.Inaddition,wethoughtthatFASplayeditsroleinfattyacidsynthesismainlyinliverpreviously.Besides,themanuscriptwasrequiredtorestrictnumberoftotalwordsandourpreviousfocuswasontheantilolyticroleofCaSRintheprocessoffataccumulation.SoweignoredtoprovidethedataofFASmRNAlevelsinthesubmittedmanuscript.Inthenewlysubmittedmanuscript,wehaveprovidedthemRNAlevelsaccordingtoyourhelpfulsuggestion.WehavereportedtheeffectsofdietarycalciumonUCP2mRNAlevelsinadiposetissueandUCP3inskeletalmuscleinourpreviousstudies(1,2).Thus,webelievedthatlowcalciumdietledtodecreasedthermogenesisinthepresentstudy.Itwasapitythatwedidnotmeasuretheratcoretemperatureinthosestudies.TheUCP2mRNAlevelsinadipocyteswereobservedtobedecreasedafter