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EpCAM倪亚平1△庄建良21．3500042．362000R735A1006-2084201221-3579-04EpCAM、、、。EpCAM、。EpCAM、。EpCAM、。ResearchProgressofCorrelationsbetweenEpCAMandNeoplasmsNIYa-ping1ZHUANGJian-li-ang2．1．FujianMedicalUniversityFuzhou350004China2．DepartmentofSurgicalOncologyQuanzhouFirstHospitalAffiliatedtoFujianMedicalUniversityQuanzhou362000ChinaAbstractEpithelialcellularadhesionmoleculeEpCAMpossessesthebiologicalfunctionsofaccelera-tingcellcyclepromotingcellproliferationdifferentiationmigrationandimmuneescape．TheresearchandexploitationoflatesttechnologyanddrugonaccountofEpCAMspecificitymonoclonalantibodycankilltumorcellshighlyeffectivelyprecislyandspecifically．RecencyresearchindicatesEpCAMasascreeningmarkeroftumorstemcellaswellasotherco-markerspossessesthecharacteristicsself-renewalhighoncoge-nicityandhereditarystability．AccordinglyasanewtypeoftumormarkerEpCAMisnotonlyutilizedintumorearlydiagnosistreatmentandprognosisjudgmentbutalsointheoreticalstudyoftumorstemcelles-tablishingthefoundationofmolecularbiologyresearchoftumorstemcell．KeywordsTumorPrognosisTreatmentStemcellEpithelialcellularadhesionmoleculeepithelialcellularadhesionmoleculeEpCAMCD326EpCAM。CD326CD133、CD44、CD24。EpCAM、、。1EpCAM、EpCAMEGP40、TROP-1、GA733-22p21GA733-24×104Ⅰ。TACSTD11。EpCAM14kbmRNA1．5kb。EpCAM80%82%2。EpCAM、。2EGF3。。α-actinin。EpCAMEpCAM。EpCAM。EpCAMEpCAM。EpCAM、、、。Litvinov4EpCAMcDNAL。EpCAMEEpCAM。EpCAME。EpCAMEEpCAME。Munz5EpCAMC-mycA、E。Osta6EpCAMRNAEpCAM。EpCAMTh2ⅡCD4+TCD4+T7-8。EpCAMEpCAMhigh/CD44+9。10EpCAM·9753·2012111821MedicalRecapitulateNov．2012Vol．18No．21p53EpCAMp53-p21。2EpCAM“”、、。EpCAM、、。Yamashita11Wnt-β-cateninEpCAMβ-cateninTcf/β-cateninβ-cateninEpCAMEpCAMWnt-β-cateninWnt-β-cateninEpCAM。12EpCAMBarrett。EpCAMEpCAMEPICD26Wnt-β-catenin13。EpCAM。EpCAMsiRNA。3EpCAM、3．1EpCAM。EpCAM。。EpCAM、、14。Kloor15EpCAM26Msh2Lynch4Msh2EpCAMEpCAMEpCAM。Msh2EpCAMEpCAM2EpCAM。EpCAMEpCAMEpCAMEpCAMLynch。Zhang16RT-PCR60、EpCAM-mRNA、75．0%81．6%55．6%67．3%。EpCAM-mRNA、RT-PCREpCAM-mRNA。3．2EpCAMEpCAM。EpCAM、EpCAM。Osta6RT-PCREpCAM-mRNA100～1000。EpCAMRNAsiRNAEpCAM35%～80%。EpCAMsiRNAMDA-MB-23191．8%96．4%。EpCAMsiRNA。Du17EpCAMRT-PCRWesternblot。siRNAEpCAM、。EpCAMEpCAM。3．2．1。EdrecolomabEDEpCAMIgG217-1A。。EDⅡ、Ⅲ18。EpCAMIgG1AdecatumumabMT201。ED·0853·2012111821MedicalRecapitulateNov．2012Vol．18No．21、、、。Schmidt19ⅡEpCAM。MT201EpCAMMT201。Ⅱ、Ⅲ。3．2．2Brischwein20EpCAM/CD3MTl10CD8+T、、MTl10。MTl10T。MTl10、EpCAM。MTl10、、、、、。Ⅰ21MTl10EpCAM、、、、。MTl10MTl10。MTl10Ⅱ、Ⅲ。Cioffi22MTl10MTl10TT。MTl10。MTl10EpCAM。TMTl10。CatumaxomabEpCAM、CD3FC。CatumaxomabT、、、。。CatumaxomabⅠ、Ⅱ、Ⅲ、、、、。CatumaxomabⅠ、Ⅱ。Strhlein23CatumaxomabⅠ10～200μg2Ca-tumaxomab。Parsons24CatumaxomabⅡ、ⅢEpCAM258Catumaxomab。。。。Catumaxomab。。Heiss25CatumaxomabⅡ、ⅢEpCAM24515788Catumaxomab。6Cox、、。28．9%6．7%HR=0．649P=0．0219。＞13%/41/131d637．0%vs5．2%。CatumaxomabEpCAM＞13%。3．2．3·1853·2012111821MedicalRecapitulateNov．2012Vol．18No．21。IGN101/2-0117-1AEpCAM+。Ⅰ、ⅡⅢEpCAM+26。Neidhart27EpCAMA11-7EpCAM-。4EpCAM、、、、。EpCAM、。EpCAM。EpCAM。1CalabreseGCrescenziCMorizioEetal．AssignmentofTAC-STD1aliasTROP1M4S1tohumanchromosome2p21andrefine-mentofmappingofTACSTD2aliasTROP2M1S1tohumanchromosome1p32byinsituhybridizationJ．CytogenetCellGen-et2001921/2164-165．2TrzpisMMcLaughlinPMPopaERetal．EpCAMhomologuesex-hibitepithelial-specificbutdifferentexpressionpatternsinthekid-neyJ．TransgenicRes2008172229-238．3ChongJMSpeicherDW．Determinationofdisulfidebondassign-mentsandN2glycosylationsitesofthehumangastrointestinalcarci-nomaantigenGA73322CO1721AEGPKS1/4KSAandEp-CAMJ．JBiolChem200127685804-5813．4LitvinovSVVeldersMPBakkerHAetal．EpCAMahumanepi-thelialantigenisahomophiliccell-celladhesionmoleculeJ．JCellBiol19941252437-446．5MunzMKieuCMackBetal．Thecarcinoma-associatedantigenEpCAMupregulatesc-mycandinducescellproliferationJ．On-cogene200423345748-5758．6OstaWAChenYMikhitarianKetal．EpCAMisoverexpressedinbreastcancerandisapotentialtargetforbreastcancergenetherapyJ．CancerRes200464165818-5824．7ZieglerAHeidenreichRBraumüllerHetal．EpCAMahumantumor-associatedantigenpromotesTh2developmentandtumorim-muneevasionJ．Blood2009113153494-3502．8GutzmerRLiWSutterwalaSetal．Atumorassociatedglycopro-teinthatblocksMHCclassⅡ-dependentantigenpresentationbydendriticcellsJ．JImmunol200417321023-1032．9DyllaSJBevigliaLParkIKetal．ColorectalcancerstemcellsareenrichedinxenogeneictumorsfollowingchemotherapyJ．PLoSOne200836e2428．10HuangHPChenPHYuCYetal．Epithelialcelladhesionmole-culeEpCAMcomplexproteinspromotetranscriptionfactor-medi-atedpluripotencyreprogrammingJ．JBiolChem20112863833520-33532．11YamashitaTBudhuAForguesMetal．ActivationofhepaticstemcellmarkerEpCAMbyWnt-beta-cateninsignalinginhepatocellu-larcarcinomaJ．CancerRes2007672210831-10839．12WongNAWarrenBFPirisJetal．EpCAMandgpA33aremark-ersofBarrett'smetaplasiaJ．JClinPathol2006593260-263．13JaenischRYoungR．Stemcellsthemolecularcircuitryofpluripo-tencyandnuclearreprogrammingJ．Cell20081324567-582．14SeligsonDBPantuckAJLiuXetal．Epithelialcelladhesionmol-eculeKSAexpressionpathobiologyanditsroleasanindepend-entpredictorofsurvivalinrenalcellcarcinomaJ．ClinCancerRes20041082659-2669．15KloorMVoigtAYSchackertHKetal．AnalysisofEPCAMpro-teinexpr