全胚胎体外培养技术的建立及评价

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HCNIESEJOURNALFONAATOMVYol.16No.419939193164(,lX)31).,·。r。ywohwoleembryoeulture,,。,,,。、,.、、1[,21。11.1.:iWsetr,,2009。(2):①:NCP。②:。③:。1.2(l):iWster,,,12,,2)/,5。,,.56℃30,,20℃。(2):31,,,,12:000.5。9.5,r,,fRiehcert’s,、。Zm!,2。,,37.5℃,100%,,1520。5%,48,5%、40%、55%。72,,、(1),O5,7。0,1,。(3):.95、10.5、11.5,、、、、、295、、.(4)90%10%Hank,s:9.590%10%aHkn,s,24、48、72,..(5):、,.0℃.Ddfo131.l,()。l2345IInI、nmn,!0~67~13142021~2728353622.12。.223o2.390%10%aHnk,s4。.24,、、、、、.,24.,,_..2,X.5s9.510.511.512.51llll0l00000.450.103.360.1.550.212.0.10.3.0012.300.!54.000.004.00.000.3000.00sssss000030.7210.1.0.010.730.14011.00.23157.7032.4.0.172.0.102.X()0.1321.00.59354.5345.115.000.00.3000.00100.0003.340.5955.323.19296.,.3()s02.30.153.10283.60.16XSsss9.510.511.512.5llll10l000.50.181.30.152.50.1701.90.102.10.232.50.17s0100(X)4.10.285.00.())s08.30.15220.202.70.150038.72】869011、40.22156.3234、10180.2021.60.65252.2050.173.40.1629.30.42430.3157.224`09%10%Hank`s))s{X5s01.80.004.40165.00.(s}、sss9.510.511.512.5lll1l0l00008.6()0.162.10.102.200.101.200、133、0O、2!2.400162.550.133.00.132.670.1300038.7218.690.700.1501130.21!51.3935.192400.222.000.0022.90.462.3751.292.670.132.530.1330.10.4()44.6356.0190%10%Hank,s,,72,。39.5,,,,,。.;。,24,,,72,。72,、,,、、。1.NewDAT.Wholeelllbyroeultuerandthestudyofammmaliaembyrosduirngorganogenesis.BioleRv.1978,53:81.2.SadlerTW,Warner(、V,Useofwholeembyroeul·tuerforevaluatingtoxicityandteratogenicity.PhanacolRev,1984,36:14553.BardfodrM.AmPidandsensitivemethodforthe297quantitationof。,rqauntitiesofPortdniltpirlPeofportdnesdjgn.iBco1976,72:248THETECHNIQUEOFWHOLEEMBRYOCULTURE190,iLshouorut~tofObsettricsdyGcolySeckriital,eBrhi,e”iytofMeealScices,hCaeh,1341)1`tecinqofoleo.byrotthatgyartbyrOSatSPItedformtheuterusotcutttleandcultuxlintm.Thenriuln.5serunitrfui」immedeltyafterthar.1formthed。normt,tina`tivatdein56℃.terabthfor30nutesand”.hecultbottleiflldeths%、forif48bo、tbenhts%cq,40%qforanother24h~.rngcult,thecultobttleorattdecontinuouy15tillPernute.tAthe72thhourobVrdethedeveloPntofernbryosthroughthe,olkacs,d`atinoratitohorL’txIt,neuarltube,vialrares,ofdihdnilmostesandottalptdnofbo.Thorughcoosnofthedevelntoft。bryoinvivoandinvitor,ofdthatd-urigntifrst24hooftcultthearteofthedeVeloPIY”tofo.bryoslarinobthconidtions,buter48hoofthecultthed。loPnntofb0invitor。rthaninvivo.tAthe72thhouroftcult,tidfltiationandfon。tlouofnoargs”tofenyros~。leinbothcon-idtions.Theoleonb0eult15agxlrI.thodinnal℃hofembyrology,teartulogy,tocolo·,,,298

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