2013,21(6):765–768Doi:10.3724/SP.J.1003.2013.04133BiodiversityScience——————————————————:2013-05-31;:2013-08-22:(31200403)Authorforcorrespondence.E-mail:lai@ibcas.ac.cnCanoco5*(,100093):(ordination),CanocoCanoco4.52002,,,Canoco4.5Canoco4.5,Canoco5201210Canoco5Canoco4.5,,(PCNMNMDS)Canoco5,,:,,,,Canoco5:anewversionofanecologicalmultivariatedataordinationprogramJiangshanLai*StateKeyLaboratoryofVegetationandEnvironmentalChange,InstituteofBotany,ChineseAcademyofSciences,Beijing100093Abstract:Ordinationofmultidimensionaldataoncommunitycompositionisoneofthemostimportantmultivariatestatisticalmethodsusedinbiodiversityresearch.Theaimofordinationistovisualizemultidimensionaldatastructureatalow-dimensionalordinationspace.CanocoisoneofthemostpopularprogramsforordinationanalysisandCanoco4.5waswidelyusedforsuchanalysisafteritsreleasein2002,becauseofitssimpleuserinterfaceandpowerfulgraphictools.AnewversionofCannoco,Canoco5wasreleasedinOctober2012.Thisnewversionsimplifiesdataentry,providesabetterhelpsystemandgraphicstools,simplifiesstepsofvariationpartitioningandsignificancetests,addssomenewmethods(e.g.PCNM,NMDS,associationanalysisoffunctionaltraits,etc.).ThispaperprovidesanoverviewofthemajorimprovementstoCanoco5,andaddressesimportantstepsrequiredforparticularanalyses.Keywords:variationpartitioning,PCNM,NMDS,functionaltraits,phylogenetic(),,(),,,,2,2,,,10,766BiodiversityScience2145(C210)45,,(ordination)(),,,,(,2004),,2050,,(vonWehrdenetal.,2009),:(unconstrainedordination),(constrainedordination)(principalcomponentsanalysis)(correspondenceanalysis,CA)(principalcoordinateanalysis,PCoA)(non-metricmulti-dimensionalscaling,NMDS)(redundancyanalysis,RDA)(canonicalcorrespondenceanalysis,CCA),,Canoco,canonicalcommunityordination(terBraak&Smilauer,2002),MicrocomputerPower1985Canoco1.0,6:Canoco1.0(1985),Canoco2.0(1985),Canoco3.0(1990),Canoco4.0(1998),Canoco4.5(2002),Canoco5(2012)Canoco4.52002(terBraak&Smilauer,2002),,,ISIWebofKnowledge,10(2003–2013),Canoco4.54,000Canoco4.5,,Canoco4.5,Canoco5201210(terBraak&Smilauer,2012)Canoco5Canoco4.5,,,(PCNM)(NMDS)Canoco5,,1Canoco4.55,ProgramsofCanocoforWindows()CanoDraw4.0forWindows()PrCoord()WcanoImp()CanoMerge()Canoco5,ICanoco52ExcelCanoco4.xCanoco,,Canoco4.x,ExcelWcanoImpCanocoCanoco5,ExcelCanoco4.x,IIExcelCanoco5,8,,Canoco5,(),(0,1),6:Canoco57673Canoco5,Canoco(adviser)Canoco,,(III)4Canoco5CanoDraw4.0,,JPEGTIFFPDFIV()():(1),():Project-Classifications(,,),Useselectedclassificationingraphs(2)Analysis-plotcreationoptions–General-plotenvelopsfor(sampleorspecies)(3):Graph-Scatterplots-(sampleorspecies)(4)Graph-ShowAttributeEditor,(5):Edit-Setting-Graphingoptions–Drawenvelopesasellipses,Graph-Recreatgraph,,,,:,,changeellipsessize,(,)Graph-ShowAttributeEditor5Conoco4.5,(PCoA)(db-RDA)(Conoco4.5),PrCoordCanoco5(Bray-CurtisGowerJaccard11),PCoAdb-RDAVPCoA,Canoco5NMDS6Canoco4.5,,Canoco5(VI),Canoco5:VariationPartitioningAanlyses,IX7,“Summarizeeffectsofexpl.variables”(VII),(VII)SimpleEffects(),ConditionalEffects8Canoco4.5,(),,(solution(.sol)),(Lepx&Smilauer,2003)Canoco5(VIII)9(PCNM)(principalcoordinatesofneighbormatrices,PCNM),(Borcard&Legendre,2002;Legendreetal.,768BiodiversityScience212005,2008;vonWehrdenetal.,2009)Canoco5(variationpartitioninganalyses)IXPCNM10,,(Petchey&Gaston,2006),Canoco5,,,Canoco5Desdevises,Newick,PatristicCanoco5,RLQXCanoco5:MicrocomputerPowerRichardE.FurnasCanoco5Canoco5Richard(FurnasR@microcomputerpower.com)BorcardD,LegendreP(2002)All-scalespatialanalysisofecologicaldatabymeansofprincipalcoordinatesofneighbourmatrices.EcologicalModelling,153,51–68.LegendreP,BorcardD,Peres-NetoPR(2005)Analyzingbetadiversity:partitioningthespatialvariationofcommunitycompositiondata.EcologicalMonographs,75,435–450.LegendreP,BorcardD,Peres-NetoPR(2008)Analyzingorexplainingbetadiversity?Comment.Ecology,89,3238–3244.LepxJ,SmilauerP(2003)MultivariateAnalysisofEcologicalDataUsingCANOCO.CambridgeUniversityPress,Cambridge.PetcheyOL,GastonKJ(2006)Functionaldiversity:backtobasicsandlookingforward.EcologyLetters,9,741–758.terBraakCJF,SmilauerP(2002)CANOCOReferenceManualandCanoDrawforWindowsUser'sGuide:SoftwareforCanonicalCommunityOrdination.Version4.5.MicrocomputerPower,Ithaca,NewYork.terBraakCJF,SmilauerP(2012)CANOCOReferenceManualandUser'sGuide:SoftwareforOrdination.Version5.MicrocomputerPowerIthaca,NewYork.vonWehrdenH,HanspachJ,BruelheideH,WescheK(2009)Pluralismanddiversity:trendsintheuseandapplicationofordinationmethods1990–2007.JournalofVegetationScience,20,695–705.ZhangJT()(2004)QuantitativeEcology().SciencePress,Beijing.(inChinese)(:)ICanoco5()IIExcelCanoco5()IIICanoco()IV()VPCoA()VI()VII(