酶切位点的保护碱基原则

EnzymeBasePairsfromend1bp2bp3bp4bp5bpAciI-+++++++AgeI+++++++++++++++AgeI-HF™++++++++++++++AluI-++++++++++++ApaI+++++++++++++++AscI+++++++++++++++AvrII+++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpBamHI++++++++++++BamHI-HF®+++++++++++BglII++++++++++++++BmtI+++++++++++++++BmtI-HF®+++++++++++++++BsaI+++++++++++++++BsaI-HF®+++++++++++++++BsiWI++++++++++++++BsmBI+++++++++++++++BsrGI+++++++++++++++BssHII+++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpClaI--+++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpDdeI+++++++++++++++DpnI-++++ntntDraIII+++++++++++++++DraIII-HF®+++++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpEagI++++++++++++++EagI-HF®+++++++++++++EcoRI+++++++++EcoRI-HF®++++++++++EcoRV+++++++++++EcoRV-HF®++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpFseI++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpHindIII-++++++++++HindIII-HF®-++++++++++HpaI+++++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpKpnI+++++++++++++KpnI-HF®+++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpMfeI++++++++++++MfeI-HF®++++++++++++MluI++++++++++++MseI+++++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpNcoI-+++++++++++NcoI-HF®++++++++++++NdeI+++++++++++NheI++++++++++++NheI-HF®+++++++++++++NlaIII++++++++++++++NotI++++++++++NotI-HF®++++++++++NsiI+++++++++++NspI--+++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpPacI+++++++++++++++PciI+++++++++++++++PmeI+++++++++++++++PstI+++++++++++++PstI-HF®++++++++++++++PvuI++++++++++++++PvuI-HF®+++++++++++++++PvuII++++++++++++PvuII-HF®-++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpRsaI+++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpSacI-+++++++++++SacI-HF®-++++++++++SacII+++++++++++++++SalI-+++++++++++SalI-HF®-+++++++++++SapI+++++++++++++++Sau3AI+++++++++++++++SbfI++++++++++++++SbfI-HF®++++++++++++++ScaI+++++++++++++++ScaI-HF®+++++++++++++SfiI+++++++++++++++SmaI+++++++++++++++SpeI+++++++++SpeI-HF®+++++++++SphI-HF®+++++++++++++SphI+++++++++++++++SspI+++++++++++++SspI-HF®+++++++++++++StuI+++++++++++++++StyI++++++++++++StyI-HF®+++++++++++++EnzymeBacktotopBasePairsfromend1bp2bp3bp4bp5bpXbaI++++++++++XhoI++++++++++++XmaI+++++++++++++++