表3在Genebank中同部分标准菌株ITS序列同源性比较结果

将0712-2序列在NCBI网站中使用blast程序在Genebank/EMBL/DDBJ基因库中进行同源性搜索,所得结果中相似性最高的前10个标准菌株比较结果见表1。从表1可见,J0712-2与雅致放射状毛霉ITS序列相似性高达99%;且全部为雅致放射状毛霉菌株。因此,确定毛霉J0712-2为雅致放射状毛霉。表1在Genebank中同部分标准菌株ITS序列同源性比较结果Tab.1ThehomologycomparisonresultofITSsequencewithpartstandardstrain菌株名称菌株号序列号相似性ActinomucorelegansstrainATCC5237014857814799%ActinomucorelegansstrainATCC4612314857814599%ActinomucorelegansstrainAc-1164632582699%ActinomucorelegansstrainATCC2296314857814499%ActinomucorelegansstrainCBS1115623217077699%ActinomucorelegansstrainSCD1223776960099%ActinomucorelegansstrainUAMH1093120936344099%MucormucedostrainMHC-7-1012114433099%MucormucedostrainMHC-7-112114432999%ActinomucorelegansstrainAc-34632582499%系统发育树构建综合同源性比对结果和雅致放射毛霉菌株在真菌发育系统中的位置,从Genebank中选择10个标准菌株的ITS序列,结果见表2;应用MEGA4软件中Phylogeny程序绘制系统发育树,结果见图1。表2所使用的ITS序列在Genebank中的登陆号Tab.2ThepracticalITSsequencegiinGenebank菌株名称菌株号序列gi号ActinomucorelegansstrainATCC52370148578147ActinomucorelegansstrainUAMH10931209363440AmbomucorseriatoinflatusstrainSmD71-1311404309ActinomucorelegansATCC22963148578144MucorcircinelloidesstrainCBS195.6875266447MucorcircinelloidesisolateR6261277547Mucorhiemalisf.luteusstrainFSU6171175940707MucormucedostrainWA0000017113226354725MucorplumbeusstrainA162146218316Fungalsp.ENTOK073O-3223558157Pilairasp.Pi-5146706170图1结果表明,0712-2菌株与雅致放射毛霉菌株的遗传距离比其他菌株小,且J0712-2菌株和雅致放射毛霉菌株在一个分支中。由此进一步证明,J0712-2菌株为雅致放射毛霉Actinomucorelegans。MucorcircinelloidesisolateR6MucorcircinelloidesstrainCBS195.68MucorplumbeusstrainA162AmbomucorseriatoinflatusstrainSmD71-1Pilairasp.Pi-5Mucorhiemalisf.luteusstrainFSU6171MucorhiemalisstrainWA0000017113J0712-2ActinomucorelegansstrainATCC52370ActinomucorelegansstrainATCC22963ActinomucorelegansstrainUAMH10931图1系统发育树Fig.1Phylogenetictree