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四川农业大学专业学位研究生学位论文论文题目：规模化猪场伪狂犬病疫苗净化措施研究学号：z2012004研究生：刘劲松专业学位（类别）：兽医硕士研究方向（领域）：预防兽医学所在学院：动物医学院校内导师：颜其贵教授校外导师：张绍元高级兽医师提交时间：2015-10-25四川农业大学研究生处制论文独创性声明本人郑重声明：所呈交的学位论文是我个人在导师指导下进行研究工作所取得的成果。尽我所知，除了文中特别加以标注和致谢的地方外，学位论文中不包含其他个人或集体已经发表或撰写过的研究成果，也不包含为获得四川农业大学或其它教育机构的学位或证书所使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了谢意。研究生签名：2015年月日关于论文使用授权的声明本人完全了解四川农业大学有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅，可以采用影印、缩印或扫描等复制手段保存、汇编学位论文。同意四川农业大学可以用不同方式在不同媒体上发表、传播学位论文的全部或部分内容。研究生签名：2015年月日导师签名:年月日I摘要猪伪狂犬病是伪狂犬病毒（PRV）侵入机体后，导致猪群发热、神经症状及繁殖障碍一种烈性传染病。该病是我国二类烈性传染病，是造成养猪行业产生重大经济损失的疫病之一。2012年前后在我国北方与南方部分省份流行，就给养猪业造成很大的损失。本研究使用PRVgE抗体检测试剂盒筛查4个候选种猪场猪群的PRVgE抗体水平，获得PRVgE抗体阳性率较低(≤10%)的阳性猪场，作为PRV净化猪场进行试验。4个种猪场的所有种猪进行Bartha-K61株基因缺失苗免疫4周后，分批次对全部种猪(含后备种猪)、部分仔猪进行伪狂犬PRVgE抗体、PRVgB抗体进行监测，淘汰PRVgE抗体阳性猪，补免PRVgB抗体阴性猪只；间隔4周后同法监测，淘汰PRVgB抗体仍为阴性猪；通过重复检测与淘汰，保留PRVgE抗体阴性与PRVgB抗体阳性种猪，建立无PRV野毒感染的稳定健康猪群。通过采取综合防控措施，如完善生物安全措施、强化生产管理、落实消毒制度、严格执行疫苗免疫程序等净化措施的实施，供试4个种猪场伪狂犬病净化后，胎均产活仔数增加0.68头，平均初生重增加0.11kg，胎均断奶健仔数增加0.71头，断奶成活率提高0.7%。抽样监测这4个稳定健康猪群/种猪场，两年内核心一场、扩繁一场、扩繁二场和扩繁三场所有种猪的PRVgE野毒抗体阳性率从试验前的3.3%、4.8%、3.6%和4.4%均降为0，成为稳定的PRVgE野毒抗体阴性猪场，表明4个种猪场的猪伪犬病均净化成功。本研究表明，对于猪伪狂犬PRVgE抗体低阳性率(≤10%)的猪场，实施净化是可行的。该净化措施的实施不但能提升猪场生产成绩，而且能明显提升猪场经济效益，为4个供试猪场节省预防治疗费用近30万元，节省饲料费用76万元，提升猪场综合经济效益120余万元。该研究也为猪场净化其它严重影响生产的疫病提供了参考。关键词：规模化种猪场；伪狂犬病；监测；净化措施IIResearchonthePseudorabiesvaccinepurificationintheindustrializedpigfarmJinsongLiuDirectedbyProfessorQiguiYanABSTRACTThepigpseudorabiesisadeadlyinfectiousdiseasescausingswinefever,itching,neurologicalsymptomsandreproductivedisordersafterthepseudorabiesvirus(PRV)intrudesintothebody.Thediseaseistheseconddeadlyinfectiousdiseases,itisoneofthediseasestoledstoheavyeconomiclossesinthepigindustry.Around2012inthenorthernandsouthernpartofChina'sispopular,ledstogreatlossesinpigindustry.ThisstudyusedfourPRVgEantibodydetectionkittoscreenPRVgEantibodylevelsfromfourcandidatesforbreedingpigfarmpigs,obtainPRVgEantibodypositiverateislow(10%)orlesspositivepigfarms,asPRVcleaningpigtoexperiments.After4weeksofimmunity,allswinefrom4breedingpigfarmgetBartha-61genedeletionstrainsvaccine.TakePRVgEantibodiesand,PRVgBantibodiesmonitoringaboutAllofswine(includingbackupswine)andpartofthepiglets.EliminatePRVgEantibodypositivepigs,supplementaryPRVgBantibodynegativepigs.After4weekswiththesamemethodtomonitoringtheherdofswine，eliminatePRVgBantibodynegativepigs.Byrepeatingdetectionandelimination,KeepPRVgEantibodynegativeandPRVgBantibodypositivebreedingpigs,toestablishinfectedthestableandhealthywithoutPRVwildpigs.Bythecomprehensivepreventionandcontrolmeasures,Suchasperfectingbiologicalsafetymeasures,strengtheningproductionmanagement,tocarryoutthedisinfectionsystem,strictlyenforcetheimplementationofthepurificationmeasuressuchasvaccineimmunizationprograms.afterthePseudorabiesvaccinepurificationinthefourbreedingpigfarm,Everytimetheproduction,theaverageoflivecubnumberincreases0.68thanbeforepurification，theaveragebirthweight0.11kgmorethanbeforepurification,theliveweaningbornnumberincreased0.71thanbeforepurification,Survivalrate0.7%higherthanthatofbeforepurification.Samplingtomonitorthesefourstableandhealthypigs/breedingpigfarm,Intwoyears,thePRVgEwildpoisonousantibodypositiverateofallthepigsfromthecoreasⅠ,thepropagationasⅠ,thepropagationasⅡand,thepropagationasⅢfromthetestIIIbeforethe3.3%,4.8%,3.6%and4.8%werereducedtozero,becomethestablePRVgEwildpoisonousantibodynegativepigfarms,Showfourpurificationbreedingpigfarmpigpseudodogdiseasearesuccessful.Thisstudyshowsthat,forpigpseudorabiesPRVgElowantibodypositiverate(10%)orlesspigfarms,theimplementationofpurificationisfeasible.Thepurificationmeasuresnotonlycanpromotetheimplementationofthepigproductionperformance,andcansignificantlyincreasetheeconomicbenefit,Thefourselectedfarmcouldsavepreventivetreatmentcostsnearly300000yuan,tosavefeedcost760000yuan,andraisetheoveralleconomicbenefitsofmorethan120120yuanofpigfarms.Theresearchforthediseaseofpigcleaningotherseriousinfluenceproductionprovidesreference.Keywords:Large-scalebreedingpigfarm;Pseudorabies;Monitoring;purificationmeasuresIV符号说明中文全称英文全称英文缩写毫升milliliterml微升microlitreul千克Kilogrammekg克grammeg微克microgramug微微克picogrampg周weekw天dayd小时hourh分钟minturemin纳米nanometernm千碱基对kilobasepairkbp碱基对basepairbp摄氏度celsius℃V目录摘要..........................................................................................................................................................IABSTRACT..............................................................................................................................................II符号说明.................................................................................................................................................IV一、文献综述...........................................................................................................................................11伪狂犬病病原学....................................................................................................................................11.1病毒基因组.........................................