糖代谢途径全解

AcCOA：乙酰辅酶A；CIT：柠檬酸；DHAP：磷酸二羟丙酮；E4P：4-磷酸赤藓糖；F6P：6-磷酸果糖；F1,6P：1,6-二磷酸果糖；FUM：延胡索酸；GA3P：3-磷酸甘油醛；G3P：3-磷酸甘油；G6P：6-磷酸葡萄糖；G6PA：6-磷酸葡萄糖酸；α-KG：α–酮戊二酸；LAC：乳酸；MAL：苹果酸；OAA：草酰乙酸；PEP：磷酸烯醇式丙酮酸；3PG：3-磷酸甘油酸；PYR：丙酮酸；Ru5P：5-磷酸核酮糖；R5P：5-磷酸核桃；SUC：琥珀酸；SUCCoA：琥珀酰辅酶A；X5P：5-磷酸木酮糖.胞内的代谢反应方程糖酵解途径GlycolysispathwayrlGLU+ATP→G6P+ADPr2G6P→F6Pr3F6P+ATP→DHAP+G3AP+ADPr4DHAP+NADH→G3P+NADr5G3P+ADP→GLY+ATPr6GA3P+NAD+ADP→3PG+NADH+ATPr73PG→PEPr8PEP+ADP→PYR+ATP三羧酸循环Tricarboxylicacidcycler11PYR+CoA+NAD→AcCoA+NADH+CO2r12PYR+ATP+CO2→OAA+ADPr14OAA+AcCoA→ICI+CoAr15ICI+NAD→α-KG+NADH+CO2r16α-KG+CoA+NAD→SucCoA+NADH+CO2r17SucCoA+ADP+Pi→SUC+ATP+CoAr18SUC+FAD→FUM+FADH2r19FUM→MALr20MAL+NAD→OAA+NADH磷酸戊糖途径Plentosephosphatepathwayr21G6P+NADP→G6PA+NADPHr22G6PA+NADP→Ru5P+NADPH+CO2r23Ru5P→0.67X5Pr24Ru5P→0.33R5Pr25X5P+R5P→F6P+E4Pr26X5P+E4P→F6P+GA3P副产物形成By-productssynthesisr9PYR+NADH→Lactate+NADr10PYR+NADH→Ethanol+CO2+NADr13PYR→PyrExt氧化磷酸化OxidativephosphorylationNADH+0.5O2+(P/O)ADP→NAD+(P/O)ATPFADH2+0.5O2+0.5(P/O)ADP→0.5(P/O)ATP+FAD用于维持的ATP消耗ATPconsumptionformaintenanceATP→ADP+Pi生物量形成Biomassformation0.067G6P+0.064R5P+0.009GA3P+0.065PG+0.050PEP+0.176Pyr+0.095OAA+0.102KG+0.249AcCoA+1.142NADPH+0.157NAD+3.82ATP→Biomass+1.142NADP+0.157NADH+0.127CO2+3.8-2ADP图4补料发酵过程中胞浆3-磷酸甘油脱氢酶、6-磷酸葡萄糖脱氢酶、丙酮酸激酶酶活的时序变化Fig.4TimecoursesoftheactivityofctGPD,G6PDHandPYKinfed-batchculturectGPD：胞浆3-磷酸甘油脱氢酶；G6PDH：6-磷酸葡萄糖脱氢酶；PYK：丙酮酸激酶ctGPD:cytoplasmicglycerol3-phosphatedehydrogenase;G6PDH:glucose-6-phosphatedehydrogenase;PYK:pyruvatekinase