文献汇报

关于肝胆胰疾病代谢研究科研汇报ZhengzhoucentralHospital,ZhengzhouUniversity2018.05.30肝胆胰微创外科林改革文献1RolesofSphincterofOddiLaxityinBileDuctMicroenvironmentinPatientswithCholangiolithiasis:FromthePerspectiveoftheMicrobiomeandMetabolomeToappearin:JournaloftheAmericanCollegeofSurgeonsAcceptedDate:2December2015背景Bileductmicroenvironmentplayskeyrolesincholangiolithiasisoccurrence.SphincterofOddilaxity(SOL)isassociatedwithcholangiolithiasis,probablyduetoenhancedrefluxofintestinalcontentsthatchangesthemicroenvironment.However,themicroenvironmenthasnotbeencomprehensivelyinvestigated方法Patientswithcholangiolithiasiswereconsecutivelyrecruitedandtheirbilewascollectedintraoperativelyforhigh-throughputexperiments.Pyrosequencingof16SribosomalRNAgenewasperformedtocharacterizethemicrobiotainthebile.Aliquidchromatography-massspectrometry-basedmethodwasusedtoprofilebilecomposition.Clinicalmanifestation,microbiome,andbilecompositionwerecomparedbetweenpatientswithandwithoutSOL结果18patientswithSOLand27patientswithoutSOLwerefinallyincluded.PatientswithSOLshowedsevererinflammation.Bacteriainthebileductwereoverwhelminglyaerobesandfacultativeanaerobes.ProteobacteriaandFirmicuteswerethemostwidespreadphylotypes,especiallyEnterobacteriaceae.ComparedwiththosewithoutSOL,patientswithSOLpossessedmorevariedmicrobiota.IntheSOLgroup,pathobionts,suchasBilophilaandShewanellaalgaehadrichercommunitieswhileharmlessbacteriawerereduced.Metabolomicsanalysisshowedthedifferencesinbilecompositionbetweengroupsweremainlydistributedinlipidsandbileacids.Particularly,theincreasedabundanceofBilophilainvolvedintaurinemetabolismwasassociatedwithreducedcontentsoftaurinederivativesinthebileofpatientswithSOL结论AbileductmicroenvironmentwithmoreseverebacterialinfectionandstrongerlithogenicitywasfoundinpatientswithSOL.Thefindingssuggestapossiblemechanismofcholangiolithiasisandprovidethebasisforfuturestrategiesforpreventionofcholangiolithiasisrecurrence.文献2MetabolomicanalysesoffaecesrevealsmalabsorptionincirrhoticpatientsToappearin:DigestiveandLiverDiseaseAcceptedDate:4February2013背景Thestudyoffaecesoffersauniqueopportunitytoobservecooperationbetweenthemicrobiomeandthemetabolismofmammalianhosts,anessentialelementinthestudyofthehumanmetabolome.Inthepresentstudy,aglobalmetabolomicsapproachwasusedtoidentifymetabolitesdifferentiallyexcretedinthefaecesofcirrhoticpatientscomparedtocontrols.方法Seventeencirrhoticpatientsand24healthyindividualswererecruited.Faecalmetabolitesweredetectedthroughnon-targetedreversed-phaseultra-performanceliquidchromatographycoupledtoelectrosprayionizationquadrupoletime-of-flightmassspectrometry.方法Patientsandvolunteersdidnotundergoanygastrointestinalsurgeryorreceiveantibiotictreatmentduringthetwomonthspriortothestudy.Subjectswereadvisedtomaintaintheirusualdietduringthestudyperiod,andtoavoidtheintakeoffermentedfoods.结果Atotalof9215peaksweredetected.Usingunequalvariancet-tests,2393peakswereobservedwithP≤0.05,approximately74.0%ofwhichwereduetodecreasedfaecalmetaboliteconcentrationsinlivercirrhosisvs.healthycontrols.Integratingmultivariatedataanalyses,weidentifiedsixmajorgroupsofmetabolites.Relativelevelsofidentifiedmetaboliteswereasfollows:strongincreaseinlysophosphatidylcholines,aromaticaminoacids,fattyacids,andacylcarnitines,andadramaticdecreaseinbileacidsandbilepigments结论Withseverehepaticinjuryinpatientswithlivercirrhosis,malabsorptionoccursalongwithdisordersoffattyacidmetabolism,potentiallyduetochangesingutmicroflora文献3胆石症与肠道菌群的相关性临床分析西北国防医学杂志2017年5月30日文献3目的：研究胆石症患者与健康个体肠道菌群的差异，探讨胆石症与肠道菌群的相关性，为胆石症防治提供新的参考依据。方法：分别收集４０例胆石症患者和４０例正常人群的新鲜粪便，采用稀释性平板菌落计数法和快速细菌鉴定法对６种细菌（肠杆菌、肠球菌、链球菌、双歧杆菌、乳酸杆菌和葡萄球菌）进行定性和定量分析检测。结果：胆石症患者较正常人群的肠道菌群发生显著变化，其大肠杆菌、肠球菌、链球菌菌落数均比正常人群明显增高（Ｐ＜０．０５），其中链球菌的升高最为显著（Ｐ＜０．０１）；肠道双歧杆菌和乳酸杆菌的菌落数与对照组相比显著下降，差异有统计学意义（Ｐ＜０．０５）；两组葡萄球菌菌落数比较，差异无统计学意义（Ｐ＞０．０５）。结论：胆石症患者存在明显的肠道菌群失调，提示该类患者存在胃肠道健康风险，同时也提示了肠道菌群失调可能是胆囊结石形成的原因之一。方法研究对象：病例组：选择我科２０１３～２０１４年因胆囊结石择期行腹腔镜胆囊切除术患者４０例，收集其术前的粪便样本。入选的胆囊结石患者无其他代谢性疾病（如肥胖、肝硬变、糖尿病和心血管疾病等），且前３个月未服用抗生素或益生菌类药物。对照组：选择在我院体检的健康成人４０例，收集其粪便样本。统计两组的基本信息。Thanksforyourattention