病毒载体pLVX-puro

PLVX-Puro载体信息如下：pLVXpuro载体基本信息出品公司:Clontech载体名称:pLVX-Puro,pLVXpuro质粒类型:哺乳动物细胞慢病毒表达载体高拷贝/低拷贝:高拷贝启动子:CMV克隆方法:多克隆位点，限制性内切酶载体大小:8102bp5'测序引物及序列:CMV-F：CGCAAATGGGCGGTAGGCGTG3'测序引物及序列:--载体标签:--载体抗性:氨苄筛选标记:嘌呤霉素备注:含有组成型CMV启动子的慢病毒载体产品目录号:632164稳定性:/组成型:--病毒/非病毒:慢病毒pLVXpuro载体质粒图谱和多克隆位点信息pLVXpuro载体简介DescriptionpLVX-PuroisanHIV-1-based,lentiviralexpressionvector.Lentiviralparticlesderivedfromthevectorallowyoutoexpressyourgeneofinterestinvirtuallyanycelltype,evenprimarycells.Expressionofyourgeneisdrivenbytheconstitutivelyactivehumancytomegalovirusimmediateearlypromoter(PCMVIE),locatedjustupstreamofthemultiplecloningsite(MCS),allowingconstitutive,highlevelexpressionofyourproteinofinterest.pLVX-Purocontainsalloftheviralprocessingelementsnecessaryfortheproductionofreplication-incompetentlentivirus,aswellaselementstoimproveviraltiter,transgeneexpression,andoverallvectorfunction.Thewoodchuckhepatitisvirusposttranscriptionalregulatoryelement(WPRE)promotesRNAprocessingeventsandenhancesnuclearexportofviralandtransgeneRNA(1),leadingtoincreasedviraltitersfrompackagingcells,andenhancedexpressionofyourgeneofinterestintargetcells.Inaddition,thevectorincludesaRev-responseelement(RRE),whichfurtherincreasesviraltitersbyenhancingthetransportofunsplicedviralRNAoutofthenucleus(2).Finally,pLVX-Puroalsocontainsacentralpolypurinetract(cPPT)elementthatincreasesnuclearimportationoftheviralgenomeduringtargetcellinfection,resultinginimprovedvectorintegrationandmoreefficienttransduction(3).Inadditiontolentiviralelements,pLVX-Purocontainsapuromycinresistancegene(Puror)underthecontrolofthemurinephosphoglyceratekinase(PGK)promoter(PPGK)fortheselectionofstabletransductants.ThevectoralsocontainsapUCoriginofreplicationandanE.coliampicillinresistancegene(Ampr)forpropagationandselectioninbacteria.UsepLVX-PuroconstitutivelyexpressesyourgeneofinterestfromPCMVIEwhentransducedintotargetcells.Beforethevectorcanbetransducedintocells,however,itmustbetransfectedinto293TpackagingcellswithourLenti-X™HTPackagingSystem(Cat.Nos.632160and632161).Thispackagingsystemallowsyoutosafelyproducehightiter,infectious,replication-incompetent,VSV-Gpseudotypedlentiviralparticlesthatcaninfectawiderangeofcelltypes,includingnon-dividingandprimarycells(4).pLVXpuro载体序列LOCUSpLVX-Puro8102bpDNAcircularSYNDEFINITIONpLVX-PuroACCESSIONKEYWORDSSOURCEORGANISMothersequences;artificialsequences;vectors.COMMENTThisfileiscreatedbyVectorNTI|biofeng.com|FEATURESLocation/Qualifierssource1..8102/organism=pLVX-Puro/mol_type=otherDNAmisc_feature454..634/label=HIV-1_5_LTRmisc_feature454..634/label=truncHIV-1_3_LTRmisc_feature745..789/label=HIV-1_psi_packCDScomplement(1015..1476)/label=ORFframe3CDS1181..2032/label=ORFframe2misc_feature1303..1536/label=RREmisc_feature2031..2046/label=cPPTpromoter2198..2702/label=CMV_immearly_promotermisc_feature2205..2492/label=CAG_enhancermisc_feature2659..2679/label=CMV_fwd_primerpromoter2660..2729/label=CMV_promotermisc_feature2703..2722/label=pCEP_fwd_primermisc_feature2705..2729/label=LNCX_primermisc_featurecomplement(2925..2948)/label=MSCV_rev_primerCDS3170..4009/label=ORFframe2misc_feature3322..3341/label=mPGK_F_primergene3410..4009/label=puro(variant)/gene=puro(variant)misc_feature4025..4600/label=WPRECDS4113..4640/label=ORFframe3misc_feature5271..5451/label=HIV-1_5_LTRmisc_feature5271..5451/label=truncHIV-1_3_LTRpromotercomplement(5576..5594)/label=M13_reverse_primermisc_featurecomplement(5593..5615)/label=M13_pUC_rev_primerpromotercomplement(5629..5658)/label=lac_promotergenecomplement(6741..7601)/label=Ampicillin/gene=AmpicillinCDScomplement(6741..7601)/label=ORFframe1promotercomplement(7643..7671)/label=AmpR_promoterterminator7757..7888/label=SV40_PA_terminatormisc_feature7845..7864/label=EBV_rev_primerORIGIN1tggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccaca61cacaaggctacttccctgattagcagaactacacaccagggccaggggtcagatatccac121tgacctttggatggtgctacaagctagtaccagttgagccagataaggtagaagaggcca181ataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccgg241agagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgag301agctgcatccggagtacttcaagaactgctgatatcgagcttgctacaagggactttccg361ctggggactttccagggaggcgtggcctgggcgggactggggagtggcgagccctcagat421cctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctga481gcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcct541tgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctc601agacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaag661cgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacgg721caagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctaga781aggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatggg841aaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatggg901caagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggct961gtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagat1021cattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagaca1081ccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagc1141aagcggccggccgctgatcttcagacctggagga