生物基尼龙

Fromzerotohero-Productionofbio-basednylonfromrenewableresourcesusingengineeredCorynebacteriumglutamicumMainTopic1Topic2Topic3Topic4第一PPT模板网IntroductionMaterialsandMethodsResultsDiscussionMainTopic1Topic2Topic3Topic4IntroductionproblemglobalwarmingozonedepletionacidrainsmogPolyamide(PA),commonlyknownasnylon,isapolymerwithamyriadofpharmaceuticalandindustrialapplications.Chemically,thepolymerbackboneiscomposedofrepetitiveunitsofdiaminesanddicarboxylicacidsthatcontaindifferentnumbersofcarbonatoms,impartingavarietyofmaterialproperties.2019/12/21Topic1MainTopic1Topic2Topic3Topic4Withoutdoubt,ahighlypromisingbio-blockforpolyamidesis1,5-diaminopentane(cadaverine,diaminopentane),afive-carbondiamine.Inthiswork,wedescribeasustainablevaluechain,extendingfromrenewableresourcestoanovel,bio-basedpolyamidePA5.10.ThedevelopmentintegratedengineeringofC.glutamicumatthecellularandtheprocessleveltoensurefermentativesupplyofthepolyamidebuildingblock,diaminopentane.MainPageTopic1Topic2Topic3Topic4Topic5Topic2strainshakeflasksfermentertestmethodMaterialsandMethodsMainTopic1Topic2Topic3Topic4Topic5ResultTopic31.Expressionoflysinedecarboxylaseinthelysinehyper-producerC.glutamicumLYS-12providesdiaminopentaneasmajorproductLys高产戊二胺产量N-乙酰-戊二胺产量赖氨酸产量2019/12/21MainTopic1Topic2Topic3Topic4Topic5Topic3MainPageTopic1Topic2Topic3Topic5Topic3Topic42.DeletionoftheN-acetyltransferaseNCgl1469eliminatesN-acetyl-diaminopentaneasby-product.2019/12/21MainPageTopic1Topic2Topic3Topic5Topic3Topic4MainTopic1Topic2Topic3Topic4Topic33.MetabolicengineeringoftheexportersforlysineanddiaminopentaneincreasesproductyieldMainPageTopic1Topic2Topic3Topic3Topic4MainPageTopic1Topic2Topic3Topic44.ThedesignedproducerC.glutamicumDAP-16accumulates88g/Ldiaminopentaneunderindustrialfermentationconditions.Topic3菌体OD2019/12/21MainTopic1Topic2Topic3Topic4Topic35.Bio-nylonPA5.10revealsexcellentmaterialpropertiesascomparedtothoseofthepetrochemicalpolyamidesPA6andPA6.6.2019/12/21MainTopic1Topic2Topic3Topic4Topic37.Reinforcementofthebio-nylonPA5.10withglassfibersincreasesmechanicalstability.2019/12/21MainTopic1Topic2Topic3Topic4Topic41.ThesyntheticproducerC.glutamicumDAP-16enablesgooddiaminopentaneproductionfromrenewableresources2.Fromtheviewpointofmaterialperformance,bio-basedPA5.10mightreplaceconventionalpetrochemicalnylons3.Bio-basedpolyamidePA5.10–apioneeringbio-basedpolymerwithadvancedmaterialpropertiesDiscussion2019/12/21谢谢MaterialsandMethodsC.glutamicumLYS-12EscherichiacolistrainsDH5αandNM522plasmidspClikintsacBandpTcMainTopic1Topic2Topic3Topic4Topic5Topic22019/12/21MainPageTopic1Topic2Topic3Topic4Topic5Topic22019/12/21Topic1Topic2Topic3Topic4Topic3返回DAP-13DAP-14DAP-15DAP-162019/12/21fermenterMainPageTopic1Topic2Topic3Topic4Topic5Topic2Fermenter30℃baffledflasks200mLmedium8h.Cellswereharvestedbycentrifugation(8800g,5min,4℃)washedoncewithsterile5%NaCl,re-suspendedin30mLof5%NaClFeedingwasstartedataglucoseconcentrationof10g/Landadjustedsuchthattheglucoseconcentrationinthefermenterremainedabove10g/L返回