河北省大型生化分析仪上岗培训贝克曼库尔特公司杨军旗2014-9-2Outline1.Beckman现有大于800T/h仪器简介2.参数设置流程(AU&DXC)3.质控的案例及质控失控处理流程4.维护保养1.贝克曼现有测速大于800T/H产品AU系列AU2700AU5421AU5821AU6801.贝克曼现有测速大于800T/H产品DXC/LX系列DxC600DXC800LX20AUSYSTEM2、参数设置通用参数参数实验项目名称组合设置实验组设置Testname的通道号决定着测试的先后次序,而group中次序为菜单显示和打印次序!参数具体参数参数分析方法ATS+RT1TAT1T2S+R1加R2S+R1T1T2TATS+R1T1T2R2A参数测光点选择与分析方法有关与试剂的加入时间和孵育时间有关具体选择参照试剂说明书LIHISE计算项目Forexample,A/G=Alb/(TP-Alb)范围参数DynamicRangeGFPanicValueRangeRepeatDecisionRangeReflexRangeNormalRangePlPhKJflfhLH范围关于旗标成对的旗标GFBDplphLHJKYUyu成对的旗标动态范围反应吸光度危急值正常值范围复查范围S1abs结束吸光度S1abs初始吸光度参数复查参数の旗标说明书第九章错误说明校准参数定标具体参数质控参数质控具体参数3.案例用户反映:我们有二台AU5821,最近1月有一台仪器Fe失控严重,二水平质控都忽高忽低不稳定,CV超过10%,另一台仪器质控正常,CV在3%以内。请帮助解决问题。Fe质控图显示严重失控正常的Fe质控图定标曲线无异常正常的RB历史记录RB历史记录不稳定重复性实验异常受到破损搅拌棒影响的FE结果升高R1-1为破损的搅拌棒R1-1为破损的搅拌棒没有用到破损的搅拌棒,结果正常。搅拌棒破损造成搅拌棒与FE试剂反应,造成结果偏高破损点。需更换新品更换搅拌捧后,重复性实验正常。质控失控处理思路维护保养日保养周保养月保养半年保养按需保养AU5800中文操作手册.pdfLX/DXCSYSTEMDXC800MCmodel2、关于定标报告的分析1、定标的目的2、定标的原因3、定标的构成仪器反馈浓度1.装入一新批号试剂2.定标时间已到3.质控超限4.重新开机5.保养后1.仪器2.试剂3.校准品定标成功的三个标准定标参数BC术语解释必要条件准确性range期望回收值必须在范围以内精确度BacktoBack重复性差值小于限制值灵敏度span曲线的斜率两点的差值大于限定值Adv.DxCVersionE,072109Calibrator:AQUACAL1Rack:Pos:NAKCLCO2CALC1RangeLimitSmplReference-4630-223837370.412408Sample-1419-470245043115422NA:-7500to-2500SmplDeviation1151724K:-4000to-1000Reference3210-24647677533014CL:1700to5700RefDeviation116618CO2:0.0to0.70CALC:-500to45002SmplReference-4573-221737140.422427Sample-1351-468144863195441BacktoBackLimitSmplDeviation941622Reference3221-24647737613014NA:190RefDeviation109315K:80CL:1303CO2:0.05SmplReference-4590-223337280.422425CALC:140Sample-1362-469545153205453SmplDeviation711318Reference3227-24627877603028RefDeviation774124SmplReference-4587-222337340.422412Sample-1358-468745313235451SmplDeviation631520Reference3228-24647967663039RefDeviation148717SetPoint150.004.0062.000.005.00SetPointUnitsmmol/Lmmol/Lmmol/Lmmol/Lmg/dLReplicatesUsed3,43,43,42,33,4-½(2233+2223)=-2228|-(2233-2223)|=10Calibrator:AQUACAL2Rack:Pos:NAKCLCO2CALC1RangeLimitSmplReference-7705148611826448Sample-4561-96719509506NA:-10750to-5250SmplDeviation641221K:-300to2700Reference3143-24537693058CL:-1000to3000RefDeviation1210819CO2:CALC:3500to85002SmplReference-7827144911906447Sample-4689-100419659524BacktoBackLimitSmplDeviation551728Reference3137-24537753077NA:190RefDeviation77515K:80CL:1303CO2:SmplReference-7837145412076428CALC:140Sample-4712-99919849514SmplDeviation661121Reference3125-24537773086SpanLimitRefDeviation97912NA:25004K:3000SmplReference-7729149411876405CL:1700Sample-4605-95819689494CO2:SmplDeviation641221CALC:3100Reference3123-24537813089RefDeviation1512818SetPoint102.010.20109.015.0SetPointUnitsmmol/Lmmol/Lmmol/Lmg/dLReplicatesUsed2,41,32,42,3Span3189369825424049½(1486+1454)=1470(1486-1454)=32Calibrator:AQUACAL1Rack:Pos:BUNmGLUCmCREmPHOSmRunTime06:46:5906:46:5906:46:5906:46:59RunDate03/01/0503/01/0503/01/0503/01/051Rate167121719211DeltaAbsInitRead-148431498-22960-19773FinalRead4834-8243-22724-147472Rate166121719212DeltaAbsInitRead-138021601-22969-19762FinalRead5717-8056-22702-147213Rate165122321211DeltaAbsInitRead-137832937-23190-19809FinalRead5687-6692-22917-147504Rate165120921211DeltaAbsInitRead-137863581-23443-19825FinalRead5743-5969-23149-14774SetPoint80.0200.01.007.50SetPointUnitsmg/dLmg/dLmg/dLmg/dLReplicatesUsed2,31,21,33,4GLUCmCalSetPointADCRangeB/BLimitSpan1200.0500to150020460250.0075to490150Adv.DxCVersionE,072109Calibrator:AQUACAL2Rack:Pos:BUNmGLUCmCREmPHOSm1Rate202871591DeltaAbsInitRead-137823879-23545-19836FinalRead-11751869-19560-201432Rate212931580DeltaAbsInitRead-141184591-23570-19874FinalRead-15152402-19597-201763Rate212881601DeltaAbsInitRead-141174849-23559-19867FinalRead-15312776-19548-202114Rate202891580DeltaAbsInitRead-141425033-23573-19901FinalRead-15223035-19588-20212SetPoint10.050.08.000.00SetPointUnitsmg/dLmg/dLmg/dLmg/dLReplicatesUsed3,43,41,43,4Span145929138211GLUCmCalSetPointADCRangeB/BLimitSpan1200.0500to150020460250.0075to490150SPAN=½(1217+1217)-1/2(288+289)=929=460DXC600/800の参数设置血清指数/超范围重检1.血清指数/自动超范围重检DIL1缓冲液用于分光光度法血清指数测量。准确吸取样品(14微升),注入含有200微升的缓冲液的小杯中。所用样品和试剂的比率为1:14.3。系统测定340、410、470、600和670纳米处的吸光率,用一组方程式来确定定每个指数的响应值。该响应值与样品的黄疸(胆红素)、溶血(血红蛋白)和脂血含量成正比。2.用户自定义项目-15点击定义定标模式:共8种(线性,数学计算模式1,2,3,8,9及DAT,DP4)主波长:于测量所需的发色团(340,380,410,470,520,560,600,650,670,和700nm)次波长:用于校正主吸收值闪烁。不能和主波长相等。(340,380,410,470,520,560,600,650,670,和700nm)计算因子:如果未输入定标参数,需要输入校正因子(-99999.000至99999.000,除了0外)定标参数:输入几点定标及各点设置点数值定标周期:最长336小时处理参数:第一次加样:可选A,B,C仓;试剂量在125ul至327ul第二次加样:可选A,B,C仓;试剂量在6ul至75ul第三次加样:可选A,B,C仓;试剂量在6ul至75ul加样本量:3ul至40ul参数●CC试盒,每个盒上有3个试剂仓:“A仓”中最多允装入的试剂量为110mL,最少应装入的试剂量为6mL“B仓”中最多允装入的试剂量为18mL,最少应装入的试剂量是1mL“C仓”中最多允装入的试剂量为4mL,最少应装入的试剂量是0.3mL•CC部分在读取试剂空白时,反应杯中的试剂最少应为200ul•CC部分样本和试剂的总体积最大不能超过330ul•仪器的CC部份样本针最大吸样量为40ul,最小吸样量为3ul4.DXC600/800维护保养Weekly检查试剂水平更换小杯拭子清洁总蛋白和白蛋白样品杯(DxC800)清洗各探针(针外壁)清洗flowcell和样本杯CC探针清洁检查氯校正跨度Monthly•更换碱性缓冲液•清洁全部样品杯模块•清洁混合器•校正灯和感测器•更换CTS刀片维护保养Everytwomonths:CTS刀片清洗(CTS系统)更换/清洁空气滤网Everythreemonths:•更换注射器活塞Everyfourmonths•清洁MC试剂管线Halfyear•更换CAL