条件性基因打靶技术研究进展

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PharmaceuticalBiotechnology2001,8(1):5457:100528915(2001)0120054204X1(,210009;1,201300)(genetargetingtechnology),,3,,Cre2loxp,;;Cre2loxp:Q78:A,ES,,,,(conditionalgenetar2geting)/1,ES,,(1),;(2),[1]2Cre2loxpCre2loxpCrep138kd,34bp,loxp[2]loxp:loxp,v,1Cre,ES[3],(a)Recombinationbetweendirectlyrepeatedloxpsites(filledtrian2gles)leadstoexcisionoftheinterveninggenesegment,leavingoneloxpsiteonreactionproduct.Notethatexcision(largearrow)ismuchmoreefficientthanreinsertion(smallarrow).(b)Recombinationbetweenloxpsitesofoppositeorientationleadstoinversionoftheinterveninggenesegment.Fig1Cre2mediatedrecombinationreactions3,45X2000208220,,19771,.E2mail:dawn876@sina.com,,,:3.1CreCreloxpCre,WesternCre[4]knockinCre,ES[5]1CreCre,,X2GalCre[6]Gre,GreTab1TrangenicmiceexpressingCreproteinsinspecifictissuesPromoterSiteofexpression(recombinase)ReferencelckT2cellsScience1994,265:10321062crystallinEyelensProc.Natl.Acad.Sci.USA1992,89:6232CD19B2cellsNucleicAcids.Res.1997,25:1317CamKIIHippocampalmeuronsCell1996,87:1317En2Mid/hindbrainCurr.Biol.1998,8:665Mx1Ubiquitous,inducibleScience1995,269:1427WAPMammaryNucleicAcids.Res.1997,25(21):4323P2AdiposetissuueNucleicAcids.Res.1997,25:2543protamin21SpermProcNatlAcadSci.USA1997,94:14602PO,POMC,IRBPSchwanncells,pituitary,retinaNucleicAcidsRes.1997,25:868nestinEarlyembryo,mosaicexpressionCurr.Biol.1996,6:1307PECAM21EarlyembryoTrends.Res.1997,6:349CMVminimalEarlyembryoNucleicAcidsRes.1995,23:5080AdenovirusEIIEarlyembryoProcNatlAcadSciUSA.1996,93:5860hu2actinEarlyembryoNatureGenet.1998,18:136DNAGuCreT2Lck/,T[7]Cre,ShibataAPCFAP,FAP,APC,ESAPC,,Cre,80%,APC[8]Cre2loxpCre,1996,BetzDNA,CreNestin,,,CreCNS,[9],Terry1997,PECAM21CreVcam21,Vcam21[10]:(1)CreCre;(2)Cre,Creknockin,Cre[11]3.2,Cre,:(1),Cre;(2);(3)55:3.2.1Cre3.2.1.1MX1Khn,MX1Cre,(rIFN)IFN(IC)loxpDNA100%,8%,IFNCRE,,10%,IFN[12]3.2.1.21992Bujard(tet)(dox)E.ColiTn10tetVP16,tTA(tettransactivator),tetdox,tTAtetOIE,,tetdox,tetVP16,[13]Cre,,tTA,tetrTA(reversetettransactivator),tet,rTA/,,Cre[14]tTArTAMayfordCamKII,rTA,CamII,doxCamIILTPdox,[15]lacR/O,lacRVP16,,[16],[17]3.2.2CreCre(hormonebindingdomain,HBD),,Hsp90Cre,Cre,,,Cre[18],HBD,C42Hpr891HBD,[19]1996FredSablitzkyHBD,525,,172,42[20]CREER2LBDES,,CreCMV,,10%()50%()[21],CreSchwenkBCre2ERB,B,80%,[22]4,,,,,,,,,1TorresRM,KhnR.Laboratoryprotocolsforconditionalgenergeting[M].OxfordUniversityPress,1997,Oxford.2KilbyNJ,SnaithMR,MurrayJA.Site2specificrecombination:toolsforgenomeengineering[J].TrendsGenet,1993,9:4133SauerB,HendersonN.TargetedinsertionofexogenousDNAintotheeu2karyoticgenomebytheCrerecombinase[J].NewBiol,1990,2:4414SchwenkF,SauerB,KukocN,HoessR,etal.Generationofrecombinase2specficmonoclonalantibodies,abletocharacterizethepatternofCreexpres2sionincre2transgenicmousestrains[J].JImmunolMethods,1997,207:2035MllerU.Tenyearsofgenetargeting:tarfetedmousemutants,fromvectordesigntophotypeanalysis[J].MechDev,1999,82:36ZinykDL,mercerEH,HarrisE,etal.Fatemappingofthemousemid2brainconstrictionusingasite2specificrecombinationsystem[J].CirrBiol,1998,8:6657Guh,MarthJD,OrbanPC,etal.DeletionofaDNApolymerasebetagenesegmentinTcellsusingcelltype2specificgenetargeting[J].Science,1994,265:1038ShibataH,ToyamaK,ShioyaT,etal.RapidcolorectaladenomaformationinitiatedbyconditionaltargetingoftheAPCgene[J].Science,1997,278:1209BetzUA,VosshenrichCA,RajewskyK,etal.Bypassoflethalitywithmo2saicmicegeneratedbyCre2loxP2mediatedrecombinatoin[J].CurrBiol,1996,6:1307658110TerryRW,KweeL,BaldwinHS,etal.Cre2mediatedgenerationofaVCAM21nullalleleintransgenicmice[J].TrandRes,1997,6:34911RichkertRC,RoseJ,RajewskyK.Blymphocyte2specific,Cre2mediatedmutagenesisinmice[J].NucleicAcidsRes,1997,25:131712KhnR,SchwenkF,AguetM,etal.Induciblegenetargetinginmice[J].Science,1995,269:142713GossenM,BujardH.Tightcontrolofgeneexpressioninmammaliancellsbytetracycline2responsivepromoters[J].NucleicAcidsResearch,1992,89:554714St2ongeL,FurthPA,GrussP.TemporalcontroloftheCrerecombinaseintransgenicmicebyatetracyclineresponsivepromoter[J].NuckeicAcidsRes,1996,24:387515MayfordM,BachME,HuangYY,etal.ControlofmemoryformationthroughregulatedexpressionofaCamKIItrangene[J].Science,1996,274:167816RiveraVM,ClacksonT,NatesanS,etal.Ahumanizedsystemforphar2macologiccontrolofgeneexpression[J].NatureMed,1996,2:102817GingrichJR,RoderJ.Induciblegeneexpressioninthenervoussystemoftransgenicmice[J].AnnuRevNeurosci,1998,19:17718PicardD.Regulationofproteinfunctionthroughexpressionofchimaericproteins[J].CurrOpinBiotech,1994,5:51119ChristophK,FrancoisT,PaulaAM,etal.RegulationofCrerecombinaseactivitybythesyntheticsteroidRU486[J].NucleicAcidsResearch,1996,24:140420ZhangY,RiestererC,SablitzkyF,etal.Induciblesite2directedrecombina2tioninmouseembryonicstemcells[J].NucleicAcidsResearch,1996,24:54321FeilR,BrocardJ,MascrezB,etal.Ligand2activatedsitespecificrecombi2nationinmice[J].ProcNatlAcadSciUSA,1996,93:1088722SchwenF,KhnR,AngrandPO,RajewskyK,etal.Temporallyandspatiallyregulatedsomaticmutagenesisinmice[J].NucleicAcidsRes,1998,26:1427ProgressinConditionalGeneTargtingWangHong,ShenZilong,XiTao,ChengGuoxi

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