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ThermoFisherSCIENTIFICTheWorldLeaderinServingScienceQExactive日常维护ByLSMSServiceTeamThermoFisher赛默飞世尔科技客户服务热线400-650-5118800-810-5118主要内容1.硬件和软件简介1.硬件简介2.软件简介2.维护(Maintainance)1.每天要做的维护2.每周要做的维护3.每个月到季度要做到维护4.几个常规维护项目介绍3.调谐&校正(Tune&Calibration)4.常见问题排查和服务流程主要内容1.硬件和软件简介1.硬件简介2.软件简介2.维护(Maintainance)1.每天要做的维护2.每周要做的维护3.每个月到季度要做到维护4.几个常规维护项目介绍3.调谐&校正(Tune&Calibration)4.常见问题排查和服务流程Q-Exactive(ExactivePlus)硬件结构-主机和外设HPLC前级真空机械泵SogevacSV65BIFC质谱主机高纯氮（99.995%）抽速：71m3/h1.QE硬件简介-IonPath（离子轨迹）C-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceTurnerKrugerLensQ-ExactiveIonPath:API离子源C-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceAPI离子源之一：TheIonMaxSourceAPI包含ESI,APCI,HESI,APPIInterchangeableprobesIonSweeptechnologyforgreaterrobustnessESIAPCIProbeHESIIIProbeIonMaxHousingAPI离子源之二：TheNanoFlexIonSource纳喷源Needle±3~5kVSprayformLCSolventEvaporationandIonDesolvationElectrospray-BasicPrincipleIonTransferTubeQ-ExactiveIonPath:APIStack区域（Capillary，SLens&ExitLens）C-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceQ-Exactive：APIStack组成加RF的SLens（18片）ExitLensAPIStack实物Capillary/IonTransferTubeMetalBallS-LensExit-LensAPIStack结构图Heatediontransfertubein-place注意1、standby，降温到100℃再拆2、不要弄丢了石墨密封圈Q-ExactiveIonPath–IonOpticsbeforeQuadropoleC-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceIonOpticsbeforeQ：InjectFlatpole/InterflatpoleLens/BentFlatpole/TKLensTKLens90度弯曲BentFlatpoleInterflatpoleLensInjectFlatpoleInterfaltpolelensandinjectflatpoleInjectFlatpole(frontview)固定InjectFlatpole和interflatpolelens的架子interflatpolelens位于InjectFlatpole后面Q-ExactiveIonPath：IonOpticsbeforeQTKLens90度弯曲的BentFlatpoleInterflatpoleLens(notshown)Q-ExactiveIonPath:Quadropole,QuadExitLensandSplitLensC-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceQ-ExactiveIonPath：主四极杆和杆后透镜主四极杆杆后透镜QuadExitLens和SplitLensQ-ExactiveIonPath:TransferMultipoleC-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceQ-ExactiveIonPath：TransferMultipoleTransferMultipoleTransferMultipoleQ-Exactive–CtrapandHVLensStackC-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceQ-ExactiveIonPath：C-TrapandHVLens(Z-Lens)C-TrapHVLens(Z-Lens)Q-Exactive–HCDCollisionCellC-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceQ-Exactive结构：HCDHCD高能碰撞池HCD高能碰撞池Q-Exactive–OrbitrapC-TrapHVLensStackSplitLensOrbitrapS-LensCapillaryInjectflatapoleInterflatapolelensHCDCollisioncellBentFlatapoleTransferOctapoleQuadExitLensQuadropoleTKLensLens6ExitlensAPISourceTheOrbitrap主要内容1.硬件和软件简介1.硬件简介2.软件简介2.维护(Maintainance)1.每天要做的维护2.每周要做的维护3.每个月到季度要做到维护4.几个常规维护项目介绍3.调谐&校正(Tune&Calibration)4.常见问题排查和服务流程1、软件简介：Tune2.1设定质谱扫描参数设定离子源参数实时数据采集质谱图仪器状态参数工具栏按钮信息窗口图形显示窗口优化，作图窗口工具栏按钮12341–On/Standby/Off打开、保存tune文件2-六通阀(最多可以接两个)和蠕动泵的控制键3-仪器状态信息（Communication，Instrument，Performance）4-UserRole类型:可以选择Standard&Advanced两种类型.AdvancedUserRole会在Calibration中显示更多的参数。InstrumentControlScanparameterSourceparameter,源的类型能被自动识别AcquiredatadirectlyInstrumentstatus树状图树状图主要是可以更多的显示仪器的反馈值和参数等，在日常使用中，不用来设置参数。TICVariation＜10%(打校正液时）在此可以看到真空读数，分子涡轮泵转速正常为100%“Messages”and“Analysisgraph”信息窗口和图形显示窗口InstrumentMessages如果仪器报错，可在此处看到一些警告信息。AnalysisGraphs可以对某些信息采集作图，这些图可以copy出来，但不会被自动保存.LogFiles（记录文件）（自动）保存的路径为：C:\Xcalibur\system\Exactive\log每次打开Tune软件的时候,都会产生一个新的记录文件。主要内容1.硬件和软件简介1.硬件简介2.软件简介2.维护(Maintainance)1.每天要做的维护2.每周要做的维护3.每个月到季度要做到维护4.几个常规维护项目介绍3.调谐&校正(Tune&Calibration)4.常见问题排查和服务流程Exactive/QExactive每天要做的检查和维护1.检查实验室的温度(18-22℃)和相对湿度(40~60%).、2.检查真空度离子传输管没有堵时，ForeVacuum的值为1-2mbar*SourceTMPspeed100%UHV10E-10mabrUHVTMPspeed100%3.检查氮气的压力ForHCD:纯度(＞99.995%)输出压力:8±0.3bar（0.8±0.03MPa）ForSource&Vent:纯度(99%)输出压力:8±0.3bar（0.8±0.03MPa）4.每天实验完成后，冲洗HPLC和离子源*如果capillary被用垫片或者电晕针堵上，ForeVacuum值为1-2E-1mbar，并会提示pressuretoolow，capillarymaybeobstucted。每天实验完成后冲洗HPLC和离子源★冲洗色谱柱参照色谱柱的清洗方法，比如100%甲醇冲洗★清洗样品毛细管和APIprobe的步骤和设置参数如下：ESI（HESI）APCI放电电流N/A0SprayVoltage0N/ASheathGas3030AuxGas55SweepGas22VaporationTemp.--（HESI可参照实验用温度）500用液相泵输送50:50甲醇:水或100%甲醇冲洗至少15分钟，流速为0.5ml/min或其他合适的流速.将流速关闭后再等5分钟，再将仪器切换至Standby反面实例★按规程冲洗ESI,APCI,HESIProbe非常重要，否则容易在离子源区造成腐蚀或污染被腐蚀的组件反面实例不开扫描的情况下将溶剂通入离子源中容易使溶剂“返”到Probe中，造成腐蚀或污染反面实例★如长期存