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１波棱瓜籽不同溶剂提取物抗菌活性研究摘要摘要：本文采用水、乙酸乙酯、甲醇、氯仿、95%乙醇、丙酮6种不同性质的溶剂提取波棱瓜籽中具有抗菌活性的物质，采用抑菌圈法分析了不同溶剂提取物对大肠杆菌（Escherichiacoli），金黄色葡萄球菌（Staphylococcusaureus），枯草芽孢杆菌（Bacillussubtilis），白色念球菌（Tritirachiumalbum），铜绿假单胞菌（Pseudomonasaeruginosa），酵母菌（Saccharomycetes）6种菌的抑菌效果。实验表明，除水以外的其他溶剂提取物对细菌，霉菌均有抑菌活性，其中甲醇，氯仿，丙酮提取物的抑菌活性相对较好。水提取物只对枯草芽孢杆菌有抑菌活性，这6种溶剂提取物对酵母菌都没有抑菌活性。随后，采用分光光度法，测定了除水以外的5种溶剂提取物对细菌的最小抑菌浓度，结果表明水提取物、乙酸乙酯提取物、甲醇提取物，氯仿提取物，丙酮提取物对大肠杆菌的最小细抑菌浓度依次0.0625mg/ml，0.5mg/ml，0.25mg/ml，0.25mg/ml，0.5mg/ml；对金黄色葡萄球菌的最小抑菌浓度依次为0.25mg/ml，0.25mg/ml，0.5mg/ml，0.25mg/ml,0.5mg/ml；对枯草芽孢杆菌的最小抑菌浓度依次为0.25mg/ml，0.5mg/ml，0.5mg/ml，0.5mg/ml，0.5mg/ml；对铜绿假单胞菌的最小抑菌浓度依次为0.25mg/ml，0.25mg/ml，0.5mg/ml，0.25mg/ml，0.5mg/ml。关键词：波棱瓜籽；抑菌圈；光吸收值；最小抑菌浓度２StudyonAntimicrobialActivitiesofEtractionsfromHerpetospermumPedunculosumAbstract:TheantimicrobialactivitiesoftheextractionsofHerpetospermumpedunculosum,whichwereextractedbydifferentdissolvent.Distilledwater,ethylacetate,methanol,chloroform,95%ethylalcoholandacetoneareusedasdissolvent.Theresultsshowedthatthedifferentextractshaveobviousinhibitioneffectsonbacterialandmold,andithadnoobviousinhibitioneffectsonyeast.Theminimalinhibitoryconcentrationtobacterialwasdetermined.ExperimentsshowthattheminimuminhibitoryconcentrationoftheextractsfromdifferentdissolventforEscherichiacoliwere0.0625mg/ml,0.5mg/ml,0.25mg/ml,0.25mg/ml,0.5mg/ml.Theywere0.25mg/ml,0.25mg/ml,0.5mg/ml,0.25mg/ml,0.5mg/mlforStaphylococcusaureus.Theywere0.25mg/ml,0.5mg/ml,0.5mg/ml,0.5mg/ml,0.5mg/mlforBacillusstubtilis.Theywere0.25mg/ml,0.25mg/ml,0.5mg/ml,0.25mg/ml,0.5mg/mlforPseudomonasaeruginosa.keywords:SeedofHepetospermumpedunculate,Inhibition,Opticaldensity,MIC３目录摘要.........................................................................................................................１ABSTRACT................................................................................................................２第1章前言...........................................................................................................５第2章实验材料与仪器...........................................................................................７2.1实验材料...............................................................................................................................７2.1.1实验菌种介绍................................................................................................................７2.1.2实验材料来源................................................................................................................９2.2培养基...................................................................................................................................９2.3其他试剂...............................................................................................................................９2.4实验仪器...........................................................................................................................１０第3章提取方法...........................................................................................................113.1波棱瓜籽的处理...............................................................................................................１０3.2制备波棱瓜籽提取物.......................................................................................................１０第4章实验过程及结果.................................................................................................124.1提取物抑菌活性的测定...................................................................................................１２4.1.1细菌培养平板的制备................................................................................................１２4.1.2制备滤纸片................................................................................................................１２4.1.3抑菌圈法测定提取物的抑菌活性............................................................................１３4.1.3.1抑菌圈实验的实验原理............................................................错误!未定义书签。4.1.3.2抑菌圈实验的具体操作............................................................错误!未定义书签。4.2用分光光度法测定提取剂的最小抑菌浓度...................................................................１３4.2.1提取液的稀释............................................................................................................１３4.2.2培养基的配制............................................................................................................１３4.2.3菌液的制备................................................................................................................１４4.2.4菌体的培养................................................................................................................１４4.2.4.1分光光度法的实验原理............................................................错误!未定义书签。4.2.4.2分光光度法实验的具体操作....................................................错误!未定义书签。第5章结果及讨论........................................................................................................165.1实验结果...........................................................................................................................１５４5.1.1波棱瓜籽的百粒重...........................