生物化学与分子生物学PPT图片素材-ScienceSlide1

AntibodyToolkit(IgG)αhelixVimentinActinandmyosinCoiled-coilMicrotubule6nm10nm25nmNucleosomes2ACACDDR1DDR1DDR1645456CD45PTPMetabolismof5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridinephosphorylase5-FluorodeoxyuridineThymidinephosphorylaseOrotatephosphoribosyltransferaseThymidinekinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidaseUridinekinaseATPADPATPADPPRPPWesternBlotting1.ResolveproteinsamplesonSDS-PAGE6.IncubatetheblotwithchemiluminescentHRPsubstrateandexposetofilm2.ElectrophoreticallytransferfractionatedproteinsfromgelontoPVDFmembrane3.Blockthemembranewithneutralprotein(BSAormilkcasein)4.Incubatethemembranewithprimaryantibodyspecifictotargetprotein5.IncubatethemembranewithHRP-labeledsecondaryantibodyspecifictoprimaryantibody