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福建农林大学本科毕业论文论文题目：头孢克洛与中药单用及混配对大肠杆菌的抑菌效果学院：植物保护学院专业年级：制药工程2012级学号：3125102083姓名：梁小强指导教师、职称：林美珍讲师2016年4月26日福建农林大学本科毕业论文UndergraduateDissertationThesisTitle:AntibacterialeffectofCefaclorsolelyandcombinedwithChinesemedicineonEscherichiacoliCollege:CollegeofPlantProtectionSpecialtyandGrade:PhyarmacyEngineering,2012StudentNumber:3125102083Name:LiangXiaoqiangSupervisor:LINMeizhenSubmittedtime:April,2016福建农林大学本科毕业论文1目录Abstract..........................................................................................错误!未定义书签。1.引言.............................................................................................................................31.1大肠杆菌.................................................................................................................31.2头孢菌素类抗生素..................................................................................................31.3抗生素与中药联合应用.........................................................................................31.4课题背景.................................................................................................................42.材料与方法.................................................................................................................52.1实验材料..................................................................................................................52.1.1试剂.......................................................................................................................52.1.2供试菌...................................................................................................................52.1.3主要仪器...............................................................................................................52.2实验方法..................................................................................................................62.2.1大肠杆菌培养和培养液配制...............................................................................62.2.2抑菌圈法...............................................................................................................62.2.3液体培养法及吸光度测定..................................................................................72.2.4头孢克洛抑菌浓度范围初筛及抑菌性中药种类筛选......................................72.2.4.1头孢克洛抑菌浓度范围初筛...........................................................................72.2.4.2抑菌性中药种类筛选及抑菌浓度范围初筛...................................................72.2.5头孢克洛、板蓝根和复方金银花单剂的抑菌活性..........................................82.2.6头孢克洛和板蓝根及复方金银花混配的抑菌活性测定..................................93.实验结果与分析.......................................................................................................103.1头孢克洛抑菌浓度范围初筛...............................................................................103.2抑菌性中药种类筛选及抑菌浓度范围初筛........................................................103.3头孢克洛、板蓝根和复方金银花单剂的抑菌活性...........................................103.4头孢克洛和板蓝根及复方金银花混配的抑菌活性测定...................................134.讨论...........................................................................................................................14参考文献......................................................................................................................15致谢..............................................................................................................................16福建农林大学本科毕业论文2摘要：本论文同时用抑菌圈法和液体培养法比较头孢克洛与中药单用及混配对大肠杆菌的抑菌效果。采用抑菌圈法比较复方金银花颗粒、甘草口服液、双黄连口服液和板蓝根颗粒四种中药试剂对大肠杆菌的抑菌效果，结果发现仅复方金银花颗粒和板蓝根颗粒两种药物有抑制效果；头孢克洛对大肠杆菌的最小抑菌浓度MIC为13.7mg/L，板蓝根的最小抑菌浓度MIC为5.8×104mg/L，复方金银花的最小抑菌浓度MIC为5.7×104mg/L。将头孢克洛与板蓝根和复方金银花混用，最佳的混配组合是头孢克洛浓度为20mg/L与板蓝根和复方金银花最高浓度的两个组合，抑菌圈直径从6.2mm增加到18.6mm和18.8mm，具有明显的加成作用。关键词：头孢克洛，大肠杆菌，板蓝根颗粒、复方金银花颗粒、联合用药，抑菌作用Abstract:Inthispaper,whileusingtheinhibitionzonemethodandliquidculturemethodandcefaclormedicinealoneandmixedwithE.coliantibacterialeffect.Inhibitionzonemethodcomparisoncompoundhoneysucklegranules,orallicorice,SHLoralBanlangenfourtraditionalChinesemedicinesandreagentsinhibitoryeffectofE.coli,foundthatonlycompoundhoneysucklegranulesandBanlangentwodrugsinhibittheeffect;cephalosporinsCroweminimuminhibitoryconcentrationMICofE.coli13.7mg/L,RadixminimuminhibitoryconcentrationMICof5.8×104mg/L,theminimuminhibitoryconcentrationofcompoundhoneysuckleMICof5.7×104mg/L.ThecefaclorandRadixhoneysucklemixandcompound,thebestcombinationismixedcefaclorconcentrationof20mg/LincombinationwithtwoRadixandthehighestconcentrationofcompoundhoneysuckle,inhibitionzonediametersfrom6.2mmto18.6mmand18.8mm,hasasignificantadditiveeffect.Keywords:cefaclor,escherichiacoli,Radixisatidisparticle,tCompoundhoneysuckleparticles,raditionalChinesemedicine,combination,bacteriostaticaction福建农林大学本科毕业论文31.引言1.1大肠杆菌大肠杆菌学名大肠埃希氏菌（Escherichiacoli），是Escherich在1885年发现，由于当时科技水平比较低，大肠杆菌被认为是在肠道菌群正常组成部分。直到20世纪中期，众多生物学家才认识到大肠杆菌对动物和人可能会有病原性。经过验证，大肠杆菌会导致严重腹泻或者败血症。大肠杆菌是动物和人肠道需要的细菌落。正常情况，大肠杆菌并不会造成疾病，但却实致病病菌。大肠杆菌是原核生物，只有核糖体，而作为病菌，大肠杆菌生长速度极快[1]。培养大肠杆菌适合温度是33℃，12h后长势良好，正因为如此，大肠杆菌变得应用广泛，论是遗传学，还是生物学以及医学上常被作为试验用的代表性细菌。1.2头孢菌素类抗生素头孢菌素类抗生素是细菌性病害的主要治疗药物，具有抗菌范围广、作用强