安徽职业技术学院毕业论文论文题目:姓名:所属系部专业:指导教师:年月日摘要花生在贮藏中存在黄曲霉毒素超标问题,严重影响我国花生的出口。为此,安徽省科技厅在2005年的科技攻关项目中,设立名称为《万吨级仁果类休闲食品原料多元复合保鲜贮藏技术及产业化》的项目,解决贮藏中黄曲霉毒素超标的问题。本实验以安徽产花生的花生仁为研究对象,通过研究涂膜材料及种类、成膜条件、柠檬醛添加量、贮藏温度和包装等因素对贮藏中黄曲霉毒素的影响,对各因素水平进行优化组合,开发了多元复合保鲜贮藏技术从而延长了花生的保藏期,并进行中试实验,同时研究了LC-MS检测黄曲霉毒素含量的新方法,取得如下研究成果:1.壳聚糖涂膜可以有效抑制花生仁在贮藏过程中黄曲霉毒素含量的增加,且对其他营养物质无显著影响。壳聚糖的浓度、制膜液的pH值和溶解温度对膜的特性有影响。包装条件对黄曲霉毒素含量影响不大,贮藏温度对黄曲霉毒素含量影响显著。2.在涂膜材料中加入柠檬醛后,抑制黄曲霉毒素的效果更佳。3.根据实验结果,结合生产实际,得出最佳的条件为:cps150的壳聚糖+1.5%制膜液浓度+0.025%柠檬醛添加量+15℃贮藏温度。4.中试实验表明,在选定的最佳条件下,花生贮藏180天,黄曲霉毒素B1及总量均低于欧盟制定的标准,可以满足出口条件。关键词:花生;黄曲霉毒素;壳聚糖;涂膜;柠檬醛AbstractDuringpeanutsstorage,studyoninhibitingaflatoxinsofcoatingtopeanutsbyanalyzingcontentsofaflatoxins.Theresultsshowsaflatoxinscanbesuppressionbytheprocessingofcoating.Sothatcoatingwouldpostphoneitsstoragetime.Chitosancoatingwasadoptedonpeanuts.TheresultsshowedthatChitosancoatingandstoragetemperaturedidinfluencenotabilityonsuppressionaflatoxinsduringpeanutsstorage,packagedidnotinfluencenotabilityonsuppressionaflatoxinsduringpeanutsstorage.Chitosancoatingcansuppressionaflatoxinsifpeanutsstoragedin10℃,vacuumshutofflightpackage.BesidesChitosancoatingwasadoptedonpeanut,coatingwhichintegratedcitralwasadopted.Theresultsshowedthattheeffectofinhibitionaflatoxinsinpeanutcanbeincreasedbyappropriatecitral.Chitosanwith0.025﹪citralcoatingcansuppressionaflatoxinsbestifpeanutsstoragedin10℃,vacuumshutofflightpackage.Duringthestorageof180d,thecontentsofaflatoxinscanbecontrolledlowerthanthestandardofEuropeanUnion.KeyWords:Peanut;Aflatoxins;Chitosan;Coating;Citral目录第1章前言..................................................................................................................11.1问题的提出.........................................................................错误!未定义书签。1.2国内外研究现状...............................................................................................11.2.1花生贮藏霉变控制的研究......................................................................11.2.2黄曲霉毒素检测的研究............................................错误!未定义书签。1.2.3覆膜工艺的研究进展................................................错误!未定义书签。1.3研究内容与目标..................................................................错误!未定义书签。第2章覆膜工艺研究......................................................................错误!未定义书签。2.1实验材料与设备..................................................................错误!未定义书签。2.1.1实验材料...................................................................错误!未定义书签。2.1.2实验器材...................................................................错误!未定义书签。2.2实验技术路线......................................................................错误!未定义书签。2.3实验方法.............................................................................错误!未定义书签。2.3.1原料的选取...............................................................错误!未定义书签。2.3.2膜材料的选择...........................................................错误!未定义书签。2.3.3涂膜..........................................................................错误!未定义书签。2.3.4包装..........................................................................错误!未定义书签。2.3.5贮藏..........................................................................错误!未定义书签。2.3.6正交实验设计...........................................................错误!未定义书签。2.3.7中试实验...................................................................错误!未定义书签。2.4实验测定项目及方法..........................................................错误!未定义书签。2.4.1含水量的测定...........................................................错误!未定义书签。2.4.2黄曲霉毒素含量的测定............................................错误!未定义书签。2.4.3花生营养成分全分析................................................错误!未定义书签。2.5结果与分析.........................................................................错误!未定义书签。2.5.1涂膜..........................................................................错误!未定义书签。2.5.2贮藏温度...................................................................错误!未定义书签。2.5.3包装方式...................................................................错误!未定义书签。2.5.4正交实验...................................................................错误!未定义书签。2.6中试实验.............................................................................错误!未定义书签。2.6.1实验材料与设备........................................................错误!未定义书签。2.6.2实验步骤...................................................................错误!未定义书签。2.6.3实验结果与分析........................................................错误!未定义书签。第三章LC-MS检测黄曲霉毒素.......................................................错误!未定义书签。3.1材料与方法.........................................................................错误!未定义书签。3.1.1实验试剂...................................................................错误!未定义书签。3.1.2仪器.........................................................................错误!未定义书签。3.1.3色谱、质谱条件........................................................错误!未定义书签。3.1.2黄曲霉毒素提取与净化............................................错误!未定义书签。3.2结果与讨论.........................................................................错误!未定义书签。3.2.1色谱分离条件的选择................................................错误!未定义书签。3.