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MALDI-TOF-MSMatrix-AssistedLaserDesorptionIonizationTimeofFlightMassSpectrometryMassspectrometry(MS)ComponentsofaMassSpectrometerIonSourceMassAnalyzerIonDetectorDataSystemm/zRelativeabundance(%)InletIonFormationSeparationDetectionSampleIntroductionMassSpectrumNobelPrize（2002）KoichiTanaka（Japanese)Usingthelasertoimpactbiologicalmacromolecules.Matrix-AssistedLaserDesorptionIonization(MALDI)IonizationoftheanalyteAnalyte：themoleculeormixturebeinganalyzedFormgaseousionswithoutfragmentationordecompositionMatrix：smallorganicmoleculeFacilitatetheionizationprocess3,5-Dimethoxy-4-hydroxycinnamicacid(Sinapinicacid,SA)2,5-Dihydroxybenzoicacid(Gentisicacid,DHB)α-Cyano-4-hydroxycinnamicacid(CHCA)Conjugatedpisystemabsorbenergyandblasttheanalytemoleclesintogasphase.Matrix-AssistedLaserDesorptionIonization(MALDI)Thesolventevaporatefromthesamplesolutionleavingbehindasolidsamplespotonthetarget.m/zRelativeabundance(%)InletMassSpectrumIonSourceMassAnalyzerIonDetectorDataSystemamatrixsample（withdifferentcomponents）sampleplatebsampleplatecsampleplate++AnalyteionsAnalyteareionizedbyprotontransferwiththenearbymatrix.Thelaserenergyabsorbedbymatrixtransmittoanalyte.m/zRelativeabundance(%)InletMassSpectrumIonSourceMassAnalyzerIonDetectorDataSystemAcceleratesionsinahigh-voltageelectricfieldkineticenergy=½mv²ConstantamountofkineticenergySeparationofionsbymasstochargeratioSmallestionstravelfastestTimeofFlight(TOF)time-of-flighttubeDetectorSampleplateLaserm/zRelativeabundance(%)InletMassSpectrumIonSourceMassAnalyzerIonDetectorDataSystemTheFeatureofMALDISoftionizationMixedsamplesNoheatedinjectionchamberCompletemolecularionsremainintactwhenionizedMSshowsthedistributionofthedifferentparticlesPreparationandstorageofasolidsampleBeingstoredundernitrogenorargonEasytooperateandshortdetectiontimeSensitivemoleculesthatdecomposeathightemperaturesApplicationsAnalyzingbiomoleculesPeptides（多肽）Lipids（脂类）Nucleotides（核苷酸）Saccharides（糖类）Otherorganicmacromolecules.SomecompoundsDNA,RNA,orRNA-proteinconjugatesAllergensinfoodmaterialsSectioningacancerouspartsofthebrainOpen-airpaintingsonthebuildingsthatformpartofourarchitecturalheritage.Thedarkeninganddegradationofcolorscausedbyurbanpollution,aggressivemarineatmospheresandclimatechange.Organicbinders’effectoncausingtheirageinginpaintsamples(i.e.oils,proteins,waxesorgums)ManyfreshwaterecosystemsareexposedtoanthropogenicpollutionDevelopingprobestoassesshowstreamecosystemsrespondWhatextenttheyareabletodegradesyntheticorganicmatterspolymeranalysisSimpleacquisitionofthemassspectraShowmainlysingle-chargedionswithhardlyanyfragmentation(i)toselectsyntheticpolymersasprobesindegradationstudies;(ii)tocomparethestartingpolymersusingMALDI-TOFMSatlabscale;(iii)tousetheselectedpolymerprobeinrealfieldexperiments.Thankyou！