抑郁症动物模型及评价方法研究进展-薛涛

20156233ACTALABOＲATOＲIUMANIMALISSCIENTIASINICAJune2015Vol．23No．3“”[e-mail]．E-mail檵檵檵檵檵檵檵檵檵檵殝殝殝殝[e-mail]．·1121*1．6460002．100193【】depression。。。。、、。【】【】Q95－33【】A【】1005-4847201503-0321-06Doi10.3969/j．issn．1005－4847.2015.03.019ＲesearchprogressonanimalmodelsofdepressionandtheirevaluationmethodsXUETao1WULi-sha1LIUXin-min2WANGQiong1*1．LuzhouMedicalCollegeLuzhou646000China2．InstituteofMedicinalPlantDevelopmentChineseAcademyofMedicalSciencesandPekingUnionMedicalCollegeBeijing100193【Abstract】Depressionaseriouslyharmfuldiseasetopeoples’physicalandmentalhealthisprevailingworld-wide．Buttheetiologyandpathologicalmechanismisstillnotfullyuncovered．Withthedevelopmentofsocialeconomyandthepaceoflifespeedingupincreasingpressureandvulnerableemotioncausedepressionincidenceincreasedrapidly．Forthefurtherresearchondepressionstudyofdepressionmechanismandantidepressantdrugshighlydependsoneffectiveani-malmodels．Thedegreeofsimilarityofanimalmodelstohumandiseasestatusandtheaccuracyandreliabilityofevaluationmethodsofanimalmodelsdirectlyinfluencethevalueoftheresutsofexperiments．Inthisarticleweintroducethecommon-lyusedanimalmodelsofdepressiontheevaluationindicatorsatorganismorganandmolecularlevelsandtoprovidetheoreticalreferencesforthefurtherstudiesondepression．【Keywords】DepressionAnimalmodelBehaviorBiochemicalcriteriaEvaluationmethods2002、、、、“”1。9“”2020。2。、、20156233ActaLabAnimSciSinJune2015Vol．23.No．33。。14①②③④。4。1.11.1.1behavioraldespairBD。1、Porsolt3、“”。、。120～25℃6min4min2120～25℃15min25min。①、②、、③。25℃20℃“”。2“”4。1cm6min4min。5。、。6。、。1.1.2learnedhelplessnessLHSeligman71967、、。1、2300.65mA30s18～42s。3306s24s。30258。。1.1.3chronicunpredicta-blestressmodelCUMS19829Willner10。CUMS、。、、、、、、、、、。。3～5311。22320156233ActaLabAnimSciSinJune2015Vol．23No．312。、。。。1.21.2.1reserpinereversal13。5-、、。1/411/223/434142.1.2。4mg/kg15。、。1.2.2Schekunov1968。DA16.0mg/kg。。Xu16-。1.2.35-5-HTP5-HTP5-HT5-HT。175-HT。5-HTP10min5-HT。5-HTNA。18、195-HTP。1.2.4yohimbinepotentiationmodelinmice。。2036-。1.31.3.1、。2223。。1.3.2-72sSeiden2080。72swaitingbehavior24。。25、26、27、28、29、30、TryonMazeDull31。22.12.1.132320156233ActaLabAnimSciSinJune2015Vol．23.No．3。2.1.2openfieldtestOFTOFT、。32、、。2.1.333。。3d11%～2%23。24h。1h。%=/+×100%34。2.1.430min。24h2cm4cm5min。2.1.5Dulawa352005。。48h5min。2.1.6CUMS。、。。2.236。。2.2.1。37。SODMDA38。2.2.2。NE、、DA、、5-HT、39。、。2.2.3BDNFBDNF。BDNF40BDNF41。42BDNFBDNF。BDNF43BDNF44、、。2.2.4HPA--HPA。HPA42320156233ActaLabAnimSciSinJune2015Vol．23No．345。HPA46。CHL147、ω-348、GFAP49、NP50、r-GABA51。311%、、、。。、、52。。。1H．DangY．ChenX．LiuetalAntidepressanteffectsofginsengtotalsaponinsintheforcedswimmingtestandchronicmildstressmodelsofdepressionJ．ProgrNeuro-psychophar-macolBiolPsychiatry2009331417－1424．2WillnerPMitchellPJ．Thevalidityofanimalmodelsofpredis-positiontodepressionJ．BehavPharmacol2002133169－188．3PorsoltＲDLePichonMJalfreMetal．Depressionanewan-imalmodelsensitivetoantidepressanttreatmentJ．Nature19772665604730－732．4WillnerP．ValidationcriteriaforanimalmodelsofhumanmentaldisorderslearnedhelplessnessasaparadigmcaseJ．ProgNeuropsychopharmacolBiolPsychiatry1986106677－690．5．J．2010184360－393．6ＲenardCEDaillyEDavidDJetal．MonoaminemetabolismchangesfollowingthemouseforcedswimmingtestbutnotthetailsuspensiontestJ．FundamClinPharmacol2003174449－455．7SeligmanMEBeaglyG．LearnedhelplessnessintheratJ．JCompPhysiolPsychol1975882534－541．8KobayashiHIwataMMitaniHetal．ValproicacidimprovesthetoleranceforthestressinlearnedhelplessnessratsJNeu-rosciＲes201272355－363．9KalzＲJSibelM．Furtheranalysisofthespecificityofanovelanimalmodeofdepressioneffectsofanantihistaminicantipsy-choticandanxiolyticcompoundJ．PharnacolBiochemBehav198216979－982．10WillnerPTowellASampsonDetal．Ｒeductionofsucrosepreferencebychronicunpredictablemildstressanditsrestora-tionbyatricyclicantidepressantJ．PsychopharmacologyBerl198793358－364．11．J．201231157－60．12DucottetCGriebelGBelzungC．Effectsoftheselectivenon-peptidecorticotropin-releasingfactorreceptor1antagonistanta-larmininthechronicmildstressmodelofdepressioninmiceJ．ProgNeuropsychopharmacolBiolPsychiatry200327625－631．13．A．．M．19981061－1071．14FouchardFMenciuCDuflosMetal．Synthesisandpharma-cologicalevaluationofnewin-dol-3-ylalkylaminesactingaspo-tentialserotoninuptakeinhibitorsJ．Arzneimittelforschung199949296－105．15．J．2007204223－225．16XuYWangZGYouWT．Antidepressant-likeeffectoftrans-resveratrolInvolvementofserotoninandnoradrenalinesystemJ．EuroNeuropsychopharmacol2010206405－413．17．--J．200331222．18．J201315264－66．19．J．20121821268－271．20．36-J．200843181391－1394．22PandeyDKMaheshＲKumarAAetal．Anovel5-HT2Are-ceptorantagonistexhibitsantidepressant-likeeffectsinabattery52320156233ActaLabAnimSciSinJune2015Vol．23.No．3ofrodentbehaviouralassaysapproachingearly-onsetantidepres-santsJ．PharmacolBiochemBehav2010943363－373．23SatoANakagawasaiOTan-NoKetal．Effectofnon-selectivedopaminergicreceptoragonistondisruptedmaternalbehaviorinolfactorybulbectomizedmiceJ．BehavBrainＲes20102102251－256．24GronliJMurisonＲFiskeEetal．Effectsofchronicmildstressonsexualbehaviorlocomotoractivityandconsum