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670陈曦(,116029)[目的]研究柄海鞘和其他5个物种的热休克蛋白70家族的系统发育关系[方法]通过在Ensembl中下载与筛选,获得柄海鞘和其他5种生物的热休克蛋白70家族的氨基酸序列,对其进行系统发育树和基因结构分析,并阐明其系统发育关系[结果]经过筛选,共获得了来自线虫果蝇柄海鞘斑马鱼家鸡和人类6种生物的热休克蛋白70家族的33个氨基酸序列,其中25个具有明确的基因结构系统发育分析表明,系统发育树并未完全按照来源物种聚类;6种生物热休克蛋白70的氨基酸序列相当保守,但其内含子插入位点并不整齐,表明热休克蛋白70家族是一个多基因多结构的家族[结论]这6个物种均发生了基因复制事件,新产生的基因仅仅是冗余的存在,还是具有新的功能,仍需作进一步的研究热休克蛋70;柄海鞘;基因结构;序列比对S188A0517-6611(2011)02-00701-02EvolutionaryAnalysisonHeatShockProtein70FamilyfromAscidianandOther5ModelOrganismsCHENXi(LiaoningNormalUniversity,Dalian,Liaoning116029)Abstract[Objective]Thestudyamiedtoresearchthephylogeneticrelationshipofheatshockprotein70familyfromtheascidianandother5species.[Method]Theaminoacidsequencesofheatshockprotein70familyfromascidianandother5organismswereobtainedthroughdownloadingandscreeningtheonefromEnsemb,ltheirphylogenetictreeandgeneticstructurewereanalyzedandtheirphylogeneticrelationshipwasexpounded.[Result]33aminoacidsequencesofheatshockprotein70familyfromthe6kindsoftheorganismsincludingascidian,nematode,drosophila,zebrafish,chickenandhumanwereobtainedthroughscreening,amongwhich25aminoacidsequenceshadthedefinitudegenesequences.Thephylogeneticanalysisshowedthatinthephylogenetictree,theheatshockprotein70familyfailedtobeclusteredaccordingtotheirsourcespecies.Theaminoacidsequencesofheatshockprotein70fromthe6specieswerehighlyconserved,buttheirinsertionallocusofintronswerenotnea,tshowingthattheheatshockprotein70familywasafamilywithmoregeneandmorestructure.[Conclusion]These6speciesalltakenplacethegeneticreplicationeventsandthefurtherstudieswillbeneededtoestmiatewhetherthenewproducedgeneswillbeonlyredundantorwithnewfunctions.KeywordsHeatshockprotein70;Ascidian;Geneticstructure;Sequencealignment陈曦(1986-),男,安徽宣城人,硕士研究生,研究方向:比较基因组与生物信息学,Emai:lchenxi860214@gmai.lcom20101012(HSPs)1964,Ritossa2530,30min,(Puff),[1]Mormioto4,HSP90(83~90kD)HSP70(66~78kD)HSP60HSP(15~39kD)100~110kD[2],,,[3]670,11.17070NCBI,Ensemblgenomebrowser,heatshockprotein70HSP70701.2ClustalX1.81[4]70,,MEGA3.1[5]mega,MEGA3.1,,10001.3Ensemblgenomebrowser,-,ClustalX1.8170,701.4SignalP3.0[6]70,,,70MEME3.5.4[7],22.17070NCBI,Ensemblgenomebrowser,342.2570,3570(1)701,70,,670,;,70!,670,JournalofAnhuiAgr.iSc.i2011,39(2):701-702张杨林卢瑶,,!,,,,13370Fig.1Phylogenetictreefor33HSP70aminoacidsequencesbasedontheNJmethod2.363370,25-,25,70,,702.470,70,,SignalP3.070,,,,,humanHSPA12AhumanHSPA13humanHSPA12B(5),70,703670,7033,25,70,,,,[1]RITOSSAFM.AnewpuffingpatterninducedbyheatshockandDNPinDrosophila[J].Experienda,1962,18:571-573.[2]MORIMOTORI.StressProteininbiologyandMedicine[M].ColdSpringHarbor:CSHLPress,1990:1-36.[3]邬堂春,贺涵贞,张国高.热应激蛋白研究的进展与前景[J].中华预防医学杂志,2000,30(2):65-66.[4]THOMPSONJD,GIBSONTJ,PLEWNIAKF,eta.lTheClustalXWindowsinterface:flexiblestrategiesformultiplesequencealignmentaidedbyqualityanalysistools[J].NucleicAcidsRes,1997,24:4876-4882.[5]KUMARS,TAMURAK,NEIM.MEGA3:integratedsoftwareformolecularevolutionarygeneticsanalysisandsequencealignment[J].BriefBioinform,2004,5:150-163.[6]BENDTSENJD,NIELSENH,VONHEIJNEG,eta.lImprovedpredictionofsignalpeptides:SignalP3.0[J].JMolBio,l2004,340:783-795.[7]BAILEYTL,ELKANC.Fittingamixturemodelbyexpectationmaxmiizationtodiscovermotifsinbiopolymers[J].ProcIntConfIntellSystMolBio,l1994,2:28-36.(上接第668页)[6]SHATSKAYAOA,SHCHERBAKVS,CHUMAKMS,eta.lRelationshipbetweenfrequencyofautodiploidcorninbredoccurrenceandoriginofinitialstock[J].MaizeGenetCoopNewsLet,t1992,66:58.[7]CHALYKST.Greatingnewhaploidinducinglinesofmaize[J].MaizeGeneticsCooperationNewsletter,1999,73:53-54.[8]赵佐宇,谷明光.药物诱导玉米孤雌生殖获得二倍体纯系[J].遗传学报,1984,11(1):39-46.[9]母秋华,杨振棠,陈泽光,等.玉米花粉胚状体无性系的筛选及其在育种中的应用[J].试验生物学报,1983,16(2):223-227.[10]李祥昭,陈洪俭,胡文端.药物诱导玉米孤雌生殖选育自交系[J].安徽农业科学,1994,22(1):52-55.[11]李冬郁,杨宪民,滕少花.利用孤雌生殖技术选育玉米自交系和杂交种初报[J].玉米科学,2005,13(S1):23-25.[12]王宏伟,史振声,邢志远,等.药物诱导玉米孤雌生殖技术选育自交系[J].玉米科学,2007,15(3):52-54.[13]郭奕明,杨映根,郭仲琛.玉米花药培养和单倍体育种研究的新进展[J].植物学通报,2001,18(1):23-30.[14]ALATORTSEVATA,TYRNOVVS.Reproducingofhaploidanddiploidmaizeformsinvitro[J].MaizeGeneticsCooperationNewsletter,2001,75:56.[15]梁文科,戚廷香,徐尚忠.单倍体技术在玉米育种中的应用和新进展[J].玉米科学,2004,12(3):13-15.[16]汤飞宇,丁菲,王国英.从玉米传粉子房培养出单倍体植株[J].福建农林大学学报,2004(12):489-493.[17]CHASESS.Productionofhomozygousdiploidsofmaizefrommonoploids[J].AgronJ,1952,44:263-267.[18]刘志增,宋同明.玉米单倍体雌雄的自然恢复以及染色体的化学加倍[J].作物学报,2000,26(6):947-952.[19]文科,黎亮,刘玉强,等.高效生物诱导玉米单倍体及其加倍方法研究初报[J].中国农业大学学报,2006,11(5):17-20.[20]魏俊杰,张晓丽,陈梅香,等.6叶期秋水仙素注射处理玉米单倍体的加倍效果研究[J].玉米科学,2007(4):49-51.[21]CHOOTM,REINBERGSE,KASHAKJ.Applicationsofhaploidinbarleybreeding[J].PlantBreedingReviews,1985,3:219-245.[22]白守信,李翠云,张振刚,等.单倍体小麦染色体加倍研究[J].遗传学报,1979(2):230-232.[23]陈玉蓉,王培.冬小麦花粉植株的适宜越夏苗龄和染色体加倍的研究[J].河北农业大学学报,1992,15(1):4-7.[24]徐玉冰,牛维和,刘继红,等.甜菜单倍体植株染色体人工加倍技术的探讨[J].中国甜菜,1991(3):7-11.702安徽农业科学2011