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!!!!!!!!!!!!!!!!!!112#115102752530006thionineThiMWNTschitosanCHITHRPH2O2H2O20.03～5.5mmol/L0.999519µmol/LS/N=32005-06-282005-11-14No.20475068No.20575082No.2005B30101003No.0342018No.241H2O21H2O2234567～129101112carbonnanotubesCNTs1991131415ThiCNTsH2O222.1HRPCHIT99%H2O230%MWNTs90%0.1mmol/LThi0.05mol/LpH6.5PBS1%CHITCHI660AH2O2Ag/AgCl2.25µL0.5%NafionNafionGC/NafGC/Naf0.1mmol/LThiPBS10minThiGC/Naf/Thi10µL1g/LMWNTs-CHIT5µL10g/LHRP8µL4℃24hH2O2GC/Naf/Thi/MWNTs-HRP-CHIT2.30.05mol/LpH6.5PBS6mLH2O23420066FENXIHUAXUEChineseJournalofAnalyticalChemistry6801～80433.1MWNTsMWNTs1%CHIT30dMWNTs1%CHITFT-IRMWNTsMWNTs1H2O2Fig.1CyclicvaltammetriccurveoftheH2O2intheabsenceandpresenceof21mmol/LH2O21.blank2.21mmol/LH2O23.2CVH2O2CV1Ea=-0.137VIpa=-1.668µAEc=-0.212VIpc=1.668µAΔE=75mVThi21mmol/LH2O2Thi3.33.3.1210min10minThi2n=3Fig.2Theeffectofelectrostaticadsorptiontimeonpeakcurrentn=310min3.3.260mV/sThiThi60mV/sThi-3H2O2n=6CH2O2=0.1mmol/LFig.3Theeffectofworkingpotentialonresponsecurrentn=6CH2O2=0.1mmol/L3.3.30-0.1-0.22-0.3-0.4V0.1mmol/LH2O230V0～-0.1V-0.1～-0.3V-0.4V-0.22V3.3.4pHpHpH5.96.67.07.68.00.1mmol/LH2O24pH5.9～7.0pH7CHITpHpH6.5PBS3.3.5HRP2083413690.1mmol/LH2O2664pHn=6CH2O2=0.1mmol/LFig.4TheeffectofpHonresponsecurrentn=6CH2O2=0.1mmol/L3.43.4.1H2O295%6s30µmol/L～5.5mmol/LI/A=4.272×10-8+7.040×10-4C/mol/LR=0.9995Lineweaver-Burk161/Iss=1/Imax+KappM/ImaxCIssCImaxKappM1.93mmol/L0.1mmol/LH2O28RSD=5.8%=19µmol/L3.4.27d0.1mmol/LH2O264d6.3%75nA3.4.3MWNTsHRPAGC/Naf/Thi/MWNTs-HRP-CHITBGC/Naf/Thi/HRP-CHITMWNTs5CH2O2=0.5mmol/LFig.5Theamperometricresponseofdifferentcompositionofthebiosensorto0.5mmol/LH2O2AGlasscarbonGC/NaF/Thi/HRP-MWNTs-CHITBGC/NaF/Thi/HRP-CHITCGC/NaF/Thi/MWNTs-CHITCGC/Naf/Thi/MWNTs-CHITHRP0.5mmol/LH2O25IAIB+ICH2O2MWNTsHRPMWNTs17MWNTsHRP18MWNTsH2O2MWNTsH2O2H2O2MWNTs4MWNTs-CHITHRPThiH2O2MWNTsCHITHRPReferences1KulaginaNVMichaelAC.Anal.Chem.200375184875～48812HigashiNYokotaHHirakiSOzakiY.Anal.Chem.20057772272～22773ChangMCYPralleAIsacoffEYChangCJ.J.Am.Chem.Soc.20041265415392～1539330864LiuChuanzhiQianKejunYangMinliHePin'gangFangYuzhi.ChineseJ.Anal.Chem.200432129～325LuJZLauCWMorizonoMOhtaKKaiM.Anal.Chem.200173245979～59836HurdisECRomeynJH.Anal.Chem.1954262320～3257ElzanowskaHAbu-IrhayemESkrzyneckaBBirssVI.Electroanalysis2004166478～4908LiuYouqinShenHanxi.ChineseJ.Anal.Chem.200432141～459ThustMSchoningMJSchrothPMalkocUDickerCISteffenAKordosPLuthH.JournalofMolecularCatalysisBEnzymatic199971-477～8310MaoLQOsbornePGYamamotoKKatoT.Anal.Chem.200274153684～368911YuXSotzingGAPapadimitrakopoulosFRuslingJF.Anal.Chem.200375174565～457112MiaoYQTanNS.Analyst200012591591～159413LijimaS.Nature199135456～5814YeJSWenYZhangWDGanLMXuGQSheuFS.Electrochem.Commun.20046166～7015LuongJHTHrapovicSWangD.Electroanalysis200517147～5316KaminRAWillsonGS.Anal.Chem.19805281198～120517DavisJJGreenMLHHillHAOLeungYCSadlerPJSloanJXavierAVTsangSC.Inorg.Chim.Acta19982721-2261～26618ZhangMGGorskiW.J.Am.Chem.Soc.200512772058～2059AHydrogenPeroxideAmperometricBiosensorBasedonCarbonNanotubesMaiZhibin1TanXuecai12ZouXiaoyong#11SchoolofChemistry&ChemicalEngineeringSunYat-senUniversityGuangzhou5102752CollegeofChemistry&EcologicalEngineeringGuangxiUniversityforNationalitiesNanning530006AbstractAnovelhydrogenperoxidebiosensorwasconstructedbasedonthecharacteristicsofthecarbonnanotubes.Thematrixofthemulti-walledcarbonnanotubeschitosanandhorseradishperoxidasewasimmobi-lizedonthesurfaceoftheglassycarbonelectrodewhichwasmodifiedbycationicdye-thionineasamediator.Alinearrangefrom30µmol/Lto5.5mmol/Lwasobservedandthedetectionlimitwas19µmol/LwhensignaltonoiserationS/Nwas3.Thisbiosensorshowshighsensitivitygoodstabilityandreproduc-ibility.KeywordsCarbonnanotubesthioninechitosanhorseradishperoxidasehydrogenperoxideReceived28June2005accepted14November200540834