三十烷醇对极大螺旋藻生物量及生化组成的影响

281Vol.28,No.120061ACTAOCEANOLOGICASINICAJanuary2006:2004203203;:2004205231.:.:(1971),,,,,().E2mail:[e-mail][e-mail]1,2,3,3(1.,361012;2.,361005;3.,361005):Zarrouk1g/m3(LTA),(Spirulinamaxi2ma)2416%2517%,NaHCO3(2)4143%4192%;1119%1610%,2517%813%;4518%6313%;,,36%60%,30%10%.,1g/m3,39176%,,(STA)12%.,.:;;;:Q949.22+1:A:025324193(2006)01201062051(Spirulina),.,(FAO),,21[1].35,(Spirulinaplatensis)(Spirulinamaxima).10,2kt[2].,,[3,4],.,,,.[37].(Triacontanol.TA),.TA,30%[810],,,.,,,,[11].ZarroukZarrouk(NaHCO3),,,,.22.1.(271),3500lx1212.Zarrouk.2.21.Zarrouk,1Zarrouk1g/m3,2Zarrouk(NaHCO3)1g/m3.(271),3500lx,1212.57d300mm,34.HETO2CD2.5,,.112NaHCO3(g)884/gm-311/mL1000100010002.33m3(,60cm,5m2).Zarrouk,1Zarrouk1g/m3,2Zarrouk1g/m3,8g/dm31.5m3,,2633.7d300mm,34.60,.2.4(1).10mg(LTA),10mL,,3.Virsonic47530min,.TGL21630min,10000r/min,,.2mL1mol/dm3NaOH,30min,4mL,.(2).10mg(LTA),10mL,24h.(3).Folin2,2.HP8543-...24h,HP8543-630,647,664,750nm(OD),,a,b,c:Ca=11185OD664-1154OD647-01083OD630;Cb=21103OD647-5143OD664-21663OD630;Cc=24152OD630-1167OD664-71603OD647.750nm.33.1,,3,12.2,1;1,2,5d12,,,.1.,1101765g(),11126845g,2416%,21106775g,4192%.,,122517%3194%.Zarrouk125115%,Zarrouk27011:14143%.,,,Zarrouk2,NaH2CO3,,.,,,Zarrouk2,NaHCO3,,.,1g/m35g/m3,1g/3,Zarrouk,,,,,..Debata[14]TA;[13]TA;Hungarter[12]TA;[11]TA().,:TA.,,,,.3.22.TA2,1,,2.TA20715g,TA,,12%,TA,,290g,39176%.TA,,,,,TA,.,,,TA.2C.;STA.;LTA.3.32,,,,([10]TA),36%60%,,30%10%.,,,(),.2(%)37286536316715896757116826066661768801283.43,,.,126115%56%,53%,1216%517%,1313%,11916%.,11119%,1518%,818%,2813%,717%.,,.,55%61%.3(%)3023.053.028.52654.513427.561.533.02861.023422.056.031.02859.03.5a3,,1a,0136%,2a,0125%0122%..1a,1,1g/m3,a.2NaHCO3,,,a,[12,13].,,4518%6313%.34,:,1g/m3,;,,,a.,,,,,,,.:[1].[M].:,1995.3-8.[2].[J].,1994,13(4):358-364.[3].[J].,1995,3:2223.[4].[J].,1994,29(10):23-24.[5],,.[J].,1999,25(4):13-16.[6],.[J].,1998,22(2):163-169.[7],.[J].,2000,5:31-34.[8].[J].,2000,2(19):223-227.[9],,,.[J].,1987,(1):12-14.9011:[10],,.[J].,1987,(4):58-61.[11],.[J].,1989,13(2):133-137.[12]HANGARTERR,STANLEYKR.Effectoftriacontanolonplantcellculturesinvitro[J].PlantPhysiol,1978,61:855-857.[13],,,.[J].,1982(1):45-47.[14]DEBATAA,MURTYKS.Effectofgrowthregulatorsonphotosyntheticefficiency,translocation&senescenceinRice[J].IndianJour2nalofExperimentalBiology,1981,19:986-987.TheeffectsoftriacontanolonbiomassandbiochemicalcompositionofSpirulinamaximaZHANGYue2ping1,WANGDa2zhi2,GAOYa2hui3,CHENGZhao2di3(1.FujianInstituteofOceanology,Xiamen361012,China;2.EnviromentalScienceResearchCenter,XiamenUni2versity,Xiamen361005China;3.DepartmentofBiology,CollegeofLifeScience,XiamenUniversity,Xiamen361005,China)Abstract:ThebiomassofSpirulinamaximaincreased24.6%25.7%whenbeingadded1g/m3liquidtria2contanolintoZarroukculturemedium;thebiomassofSpirulinamaximastillincreased4.43%4.92%e2venifcontentofNaHCO3inthemediumwasdecreasedhalf(Test2).Thecontentofcarbohydrateincreased11.9%16.0%inTest1and5.7%8.3%inTest2respectively.Thecontentofchlorophyllaincreased45.8%63.3%;thetotalcontentofproteinkeptinvariablebasically,buthydrosolubleproteinwasobvi2ouslyimprovedfrom36%to60%orso,whilenon2hydrosolubleproteindecreasedfrom30%to10%orso.Moreover,39.76%rateofincreaseofbiomasswasgotwhenadding1g/m3liquidtriacontanolonalarge2scalecultureatSpirulinaproducebase,higherthanonalittle2scaleatlaboratory,butdecreased12%whenaddingsolidtriacontanol.Soaconclusioncanbeenobtained:theSpirulinabiomassandbio2chemicalcompositionweregreatlyimprovedwhenaddingliquidtriacontanolintotheculturemedium.Keywords:triacontanol;Spirulinamaxima;biomass;biochemicalcomposition01128