两种硫杆菌对河涌底泥重金属的生物沥滤

2008,28(7)624~629ChinaEnvironmentalScience,*(,510275)(Thiobacillusferrooxidans,T.f)(Thiobacillusthiooxidans,T.t),2CdCrPbNiCuZn6.,T.fT.t.CdCrNiCuZnT.f60%45%70%90%75%,T.t45%34%50%65%55%.Pb,25%.T.fSFe2+,2CdNiCuZn,CrPb,S.T.fSFe2+2,.T.fSO42Fe3+,,.X701A10006923(2008)07062406BioleachingofheavymetalsfrombrookletsedimentbyThiobacillusferrooxidansandThiobacillusthiooxidans.ZHANGZai-li,JIAXiao-shan*(SchoolofEnvironmentalScienceandEngineering,SunYat-senUniversity,Guangzhou510275,China).ChinaEnvironmentalScience,2008,28(7)624~629AbstractThiobacillusferrooxidans(T.f)andThiobacillusthiooxidans(T.t)obtainedwithseparationandpurifyingfromacidminewastewater.UtilizingthesetwokindsofThiobacillusonCd,Cr,Pb,Ni,Cu,Zn6kindsofheavymetalsofGuangzhouGuocunBrookletsedimentwastogoonsequencingbioleachingtest.T.fbacterialeachingoutabilityonheavymetalswashigherthanonT.tbacteria.TheleachingoutrateofT.fbacteriaonCd,Cr,Ni,Cu,Zncouldreachhighest60%,45%,70%,90%,75%,T.tbacteriahoweverwasrespectively45%,34%,50%,65%,55%.ThesetwoonPbremovalratewasmatched,allwasalmost25%.SupplyingdifferentsubstratesulfurpowderandferroustoT.fbacteria,theleachingouteffectson4kindsofmetalsCd,Ni,Cu,Zndidnothaveobviousinfluence,andonleachingoutofCr,Pb,theleachingouteffectofsulfurpowdertreatingwasmoreletter.Meanwhile,addingsulfurpowderandferroustoT.fbacteria,thusitsleachingouteffectwaslowered,mightbethatsulfateandferricT.fbacteriametabolizedreactedtoproduceM2Fe6(SO4)4(OH)12(M=K+,Na+,NH4)thatproducedco-sedimentwiththedissolvedoutheavymetals,thusloweringitsleachingouteffect.KeywordsThiobacillusferrooxidansThiobacillusthiooxidansbioleachingsedimentheavymetals50%~80%[1],..2090,,[24],[57].[810],.(Thiobacillusferrooxidans,T.f)(Thiobacilusthiooxidans,T.t)[11],2,6,.11.1T.fT.tT.f20070921985*,,[e-mail]7625,9K[12],9K[13]T.tStarkeyS[14],[15],2~3T.fT.t1.2T.fT.t16SrDNA1.2.1DNA2000r/min5min.SDSK,(24:1),DNA[16].1.2.2PCR[17]50µL.16SrDNAF27R1522[18].1.2.316SrDNAPCR.1.31.3.10.1MPa,12120min.:10%;18%;,TN27005mg/kg;TP16005mg/kg;Cd11.95mg/kg;Cr372.8mg/kg;Pb255.2mg/kg;Ni944.33mg/kg;Cu4224.65mg/kg;Zn6499.45mg/kg.1.3.2180mL500mL,2.20g/LFeSO4⋅7H2O,10g/LS.10%T.fT.t,150r/min,30,pH2.,10%,T.fT.t2.1.3.3.,FeSO4⋅7H2O,S.T.fFe2+S,3.1,T.fFeSO4⋅7H2O;2,T.fS;3,T.fFeSO4⋅7H2O+S.T.tSFe2+,T.tS,,T.tS.,5,FeSO4⋅7H2O;6,S;7,FeSO4⋅7H2O+S,8,.3.FeSO4⋅7H2O20g/L10g/L,10%(V/V),30,120r/min.pH5,FeSO4⋅7H2OpH4.0~4.5,pH,SpH4.0~4.5,FeSO4⋅7H2OpH.1,10000r/min10min,,Fe2+SO426..1.4Fe2+;pHMO130pH;SO42;.22.1T.fT.t16SrDNAT.fT.t1416b,1410b.2,GenBank,EF199808EF199809.BLAST216SrDNAGenBank/EMBL/DDBJ16SrDNA,(1,2).,T.fAcidithiobacillusferrooxidansATCC23270T,16SrDNA99%.T.fAcidithiobacillusferrooxidansstrainYTWAcidithio-bacillusferrooxidansstrainD2AcidithiobacillusferrooxidansstrainTFY,16SrDNA100%100%99%.T.tThiobacilluscaldusDSM8584T,95.2%.T.tUnculturedAcidithio-bacillussp.V1Acidithiobacillussp.ORCS6UnculturedAcidithiobacillussp.K13,16SrDNA100%97%97%.62628AcidithiobacillusferrooxidansstrainTFI(AF465606)AcidithiobacillusferrooxidansstrainTFI(AF465607)AcidithiobacillusferrooxidansstrainCMS(DQ062118)AcidithiobacillusferrooxidansN-Fe2(X75266)AcidithiobacillusferrooxidansstrainTFY(AF465608)T.fAcidithiobacillusferrooxidansstrainYTW(DQ062116)Acidithiobacillusferrooxidans(AJ278723)Acidithiobacillussp.NO-25(AF376019)Acidithiobacillussp.NO-37(AF376020)Acidithiobacillussp.KSC1(AF376017)Acidithiobacillussp.NO-8(AF376018)AcidithiobacillusferrooxidansstrainTFN-d(AF465605)AcidithiobacillusferrooxidansstrainATCC33020(AJ278719)AcidithiobacillusferrooxidansstrainSS6(AJ278721)AcidithiobacillusferrooxidansstrainBRGM1(AJ457806)AcidithiobacillusferrooxidansstrainB4(AJ879995)AcidithiobacillusferrooxidansstrainWJ67(AY495958)AcidithiobacillusferrooxidansstrainDSM9465(Y11595)AcidithiobacillusferrooxidansstrainK2(AJ278722)Thiobacillusferrooxidans(AB039820)AcidithiobacillusferrooxidansstrainWJ13(AY495953)AcidithiobacillusferrooxidansstrainATCC23270(AF465604)AcidithiobacillusferrooxidansstrainSS4(AJ278720)AcidithiobacillusferrooxidansstrainBY(DQ062117)AcidithiobacillusthiooxidansstrainWJSo(AY495961)Thiobacillussp.(X98210)6687429695629988802290656599369993950.002AcidithiobacillusthiooxidanstypestrainATCC19377(Y11596)1T.f16SrDNAFig.1Phylogenetictreeconstructedbythesequenceofthe16SrDNAofT.ferrooxidansanditsnearestneighboursinGenBankT.tUnculturedAcidithiobacillussp.V1(AF339743)Acidithiobacillussp.ORCS6(AY830897)Thiobacilluscaldus(AB023405)ThiobacilluscaldusDSM8584(Z29975)UnculturedbacteriumcloneRA13C21(AF407401)AcidithiobacillusthiooxidansstrainWJSo(AY495961)AcidithiobacillusthiooxidansB-S3(X75269)Thiobacillussp.Fras2(AF213056)AcidithiobacillusthiooxidanstypestrainATCC19377(Y11596)UnculturedAcidithiobacillussp.K13(AF460982)Acidithiobacilluscaldus(AF513711)UnculturedbacteriumcloneRA13C21(AF407402)Thiobacillussp.(X98210)99319999979961999941520.0052T.t16SrDNAFig.2Phylogenetictreeconstructedbythesequenceofthe16SrDNAofT.thiooxidansanditsnearestneighboursinGenBank2.2pHpH3.,pH.,T.fFe2+12~3d,pH2.2S24pH,14d.,pH,Fe2+57Fe2+2d,pH,3.5.pH2,.2.3Fe2+SO424,5T.fT.tFe2+S.T.fT.t1,Fe