中国致病性小肠结肠炎耶尔森菌生物分型研究

2010,26(7)ChineseJournalofZoonoses:1002-2694(2010)07-0651-03中国致病性小肠结肠炎耶尔森菌生物分型研究肖玉春,王鑫,邱海燕,景怀琦:初步了解我国致病性小肠结肠炎耶尔森菌O3O9血清型菌株的生物型分布,掌握我国致病性小肠结肠炎耶尔森菌的生物学特征用文献报道方法对1986-2008年从我国不同地区的腹泻病人家禽家畜鼠类食品环境等各类宿主中分离到的427株致病性小肠结肠炎耶尔森菌进行生物分型427株致病性小肠结肠炎耶尔森中213株属于生物2型(49.9%),全部为O9血清型;208株属于生物3型(48.7%),其中191株为O3血清型,17株为O9血清型;6株属于生物4型(1.4%),全部为O3血清型没有发现生物1A型1B型与5型的致病性菌株我国O3血清型致病性小肠结肠炎耶尔森菌以生物3型为主O9血清型致病性菌株以生物2型为主,没有生物1B型菌株,与国外致病性菌株的生物血清型分布有一定差异:致病性小肠结肠炎耶尔森菌;血清型;生物型:R378:AStudyofBiotypingforpathogenicY.enterocoliticastrainsinChinaXIAOYUchun,WANGXin,QIUHaiyan,JINGHuaiqi(InstituteofCommunicableDiseaseControlandPrevention,ChineseCenterforDiseaseControlandPreventiom,Beijing102206,China)ABSTRACT:ToanalysesthedistributionandcharacteristicsofbiotypeO3andO9ofY.enterocoliticastrainsinChina,pathogenicY.enterocoliticastrainsisolatedfromdiarrheapatients,domesticanimals,murine,foodandenvironmentwerebiotypedaccordingtoreportedmethods.Among427strains,213(49.9%)werebiotype2,208(48.7%)werebiotype3,and6(1.4%)werebiotype4,whilenobiotype1A,1Band5werefound.It'sconcludedthatinChina,biotype3wasthedominateinO3serotypeofY.enterocoliticaandbiotype2wasthedominateinO9serotype.Thereisnobiotype1BinChina,whichisdifferentfromthedistributionofpathogenicY.enterocoliticabioserotypeinothercountries.KEYWORDSPathogenicY.enterocolitica;serotype;biotype通讯作者:景怀琦,Email:[e-mail]:中国疾病预防控制中心传染病预防控制所,北京102206,,,,,(PFGE),,13!,30,11.1:,,1986-2008,427:52203(O3)52211(O8)52212(O9)O5,27D92651ChineseJournalofZoonoses2010,26(7)1.2O3O9O8,O5,27,O3O8O91.334!,,1.42!:11A1B2345++----+-----+-----++(+)---++++-d+++++-+++++-DNA----++d-----D+-----+-----:+:90%;d:11%-98%;-:90%;(+)::1%800.01%(CaCl2∀H2O)(),35#4d,()(Oxoid,),25#24h1A,;(Rosco,):,OD3.3(),1.5mLeppendorf,250L,,25#,22.1O3O8O941B2,O5,2732.2427,2132,49.9%;2083,48.7%;64,1.4%1A1B52O3O9197O3,1913,643O3(3/O3),O397.0%;O34(4/O3),3.0%230O9,2132(92.6%),173(7.4%),2O9,1B5!21A1B2345O31916O921317213208652203(O3)+52211(O8)+52212(O9)+D92(O5,27)+3,(PFGE),,606!,O36522010,26(7)ChineseJournalofZoonosesO9O5,27O8O13aO13bO20O212!586!,O3O94,7!,O8,23,5!,3061A8!(HPI)1B,O8,O211B5!234523!,23,419801B,O8,HPI(bioserotype),:∃4/O3%∃4/O3%,4/O3,1B/O8,1B/O82/O923!,3/O32/O9,4/O3,:1!CoverTLandAberRC.YersiniaenterocoliticaJ!.NEnglJMed,1989,321:1624.2!BottoneEJ.Yersiniaenterocolitica:thecharismacontinuesJ!.ClinMicrobiolRev,1997,10:257276.3!ThoernerP,BinKingombeCI,BogliStuberK,etal.PCRdetectionofvirulencegenesinYersiniaenterocoliticaandYersiniapseudotuberculosisandinvestigationofvirulencegenedistributionJ!.ApplEnvironMicrobiol,2003,69:18101816.4!WangX.,QiuH,JinD,etal.O:8serotypeYersiniaenterocoliticastrainsinChinaJ!.IntJFoodMicrobiol,2008,125:259266.5!PelludatC,RakinA,JacobiCA,etal.TheyersiniabactinbiosyntheticgeneclusterofYersiniaenterocolitica:organizationandsiderophoredependentregulationJ!.JBacteriol,1998,180:53846.6!M!.,2004.7!,,,.O:3O:9J!..2004,15:317319.8!SinghI,VirdiJS.ProductionofYersiniastabletoxin(YST)anddistributionofystgenesinbiotype1AstrainsofYersiniaenterocoliticaJ!.JMedMicrobiol.2004,53:10651068.:20100126;:20100317(上接第650页)13!StoffelsJA,LeeuwenWM,PostRJ.DetectionofplasmodiumsporozoitesinmosquitoesbypolymerasechainreactionandoligonucleotiderDNAprobe,withoutdissectionofthesalivaryglandsJ!.MedVetEntomol,1995,9(4):433437.14!MorenoM,CanoJ,NzamboS,etal.MalariapanelassayversusPCR:DetectionofnaturallyinfectedAnophelesmelasinacoastalvillageofEquatorialGuineaJ!.MalarJ,2004,3:20.15!VythilingamI,NitiavathyK,YiP,etal.Ahighlysensitive,nestedpolymerasechainreactionbasedmethodusingsimpleDNAextractiontodetectmalariasporozoitesinmosquitosJ!.SoutheastAsianJTropMedPublicHealth,1999,30(4):631635.16!TassanakajonA,BoonsaengV,WilairatP,etal.PolymerasechainreactiondetectionofPlasmodiumfalciparuminmosquitoesJ!.TransRSocTropMedHyg,1993,87(3:273275.17!GreenCA,RattanarithikulR,PongparitS,etal.Anewlyrecognizedvectorofhumanmalarialparasitesintheorientalregion,Anopheles(cellia)pseudowillmori(theobald,1910)J!.TransRSocTropMedHyg,1991,85(1):3536.18!GreenCA,RattanarithikulR,CharoensubA.PopulationgeneticconfirmationofspeciesstatusofthemalariavectorsAnopheleswillmoriandAnophelespseudowillmoriinThailandandchromosomephylogenyofthemaculatusgroupofmosquitoesJ!.MedVetEntomol,1992,6(4):335341.19!,,,.J!.,1996,3(2):100105.:20100104;:20100303653