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23,1Vol123,No1120061JOURNALOFSHENZHENUNIVERSITYSCIENCEANDENGINEERINGJan12006:100022618(2006)0120001209:2005209212;:2005211211:(60408011,60138010);(5010500);(200525):(19702),(),,.E2mail:jlqu@szu1edu1cn1,1,2,1,1,1,1(1.,518060;2.,300071):,,,.,,,.,,.:;;;;;;:O43;Q6:A1961,Franken694nm,347nm[1].(secondharmonicgenera2tion,SHG),.,SHG.,..SHG,(two2photonexcitedfluores2cence,TPEF).SHGTPEF,,,;,.,,,,,.,Shi2WeiChu115mm[2],,,.,,.,SHG.SHG,,,,.,1230nm,100mW,12h,1kJ,,[2].,,,.,SHG,()©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[3]P=0((1)E+(2)E2+(3)E3+)(1),(1);(2)(3).0(2)E2SHGTPEF.E1cos(t+),(1),120(2)E21+120(2)E21cos[2(t+)].,[4].,,,02.2,.SHG.,,,,SHG.SHG.,,,,,.,SHGdeff,deff.deff,,.,[5].,,.,SHG,.,,,1(a).1SHGFig11MomentumconservationofSHG,.,(guoyshift),,k(k,1).,SHG,arccos(),1(b).,,,,..N1(z)=N1[1+sin(k2Qz)](2)©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:3N1(qz)=N1(0)+i2((qz+Q)+(qz-Q))(3),k2(0).(3),Qk2,.Q,Qk2z,-Qk2,1(c)(1(b)k2.k2).,Q=,,;Q=1-,,;Q=1+,,.,,.EGeorgiou7601070nmI,,,1024nm,I512I1024:logI512=1192logI1024[6],.2SHGSHG,2.2(a):,,2,,(),(PMT).:[7].:Ti(80MHz),,2SHGFig12ExperimentalsetupofSHG(7001000nm),.SHG,,,[8].:,,.:,.(10nm).:,,(non2descanned).PMT.,,.,Ti:,350500nm,.,.,.[9],,.©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[10],.Freund,50mm.,SHG,[11][12]IAchilles[6][13].:[6,14215],,,.,.,,[16].,:,(),.(),,.,(RH237JPW22080),,[17220].,[21].,,,,().311,,.,,,,,.31111SHG2OCT(opticalco2herencetomography,OCT),.OCT(),SHG().,[12,22].,OCT,SHG,[23].SHGOCT.SHG;,,OCTOCT.,OCT;,OCT,,.YiJiang800nmSHG2OCT,311m[12].SHG2OCT,,.,SHG2OCT,.31112SHG2TPEFSHGTPEF,[24225].SHGTPEF,,.,(2)(2)..,,,,.SHGTPEF,,,.,,.©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:5(Alzheimer’s)[24].31113SHG.,[23][25].,,[26].,;,(2),;,[27],r=017..31114,[28],3.,.CCD.,,CCD..,3ms/,,.3[28]Fig13SHIMofmulti2focus312.,:.[11,22],[19].31211SHG,SHG,,.,.,[3,19220].,Andrew[29]SHGTPEF,.31212SHG,.,,,,.4,(hamstercheekpouchmucosaltissue),,hamstercheekpouch[22],.4[22]Fig14Experimentalsetup,SHG.EdwinLSteele(MGH/HMS)eGFP,C6001GM6001.©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[17],SHG.1999,PJCampagnola[3]SHG.,850nm,SHG18/100mV,TPEF10/100mV[18].2004,Andrew.,850910nm,di242ANEPPSdi242ANEPMPOHSHG20/100mV,,950970nm,40/100mV[20].SHG.,Cornell,DHPESBP,(5),[19].,Alzheimerps,.5DHPESBP,[19]Fig15SHGIofneuronstainedwithDHPESBP,,.,SHG.,,.,,.,.20nm[30],.,,,,.,,.:[1]FrankenPA,HillAE,PetersCW,.[J].,1961,7:1182119().[2]ChuShi2wei,ChenSzuYu,TsungHanTsai,.[J].,2003,11(23):309323099().[3]PaulJCampagnola,MeideWei,AaronLEwis,.SGH[J].,1999,77(6):334123349().[4],.[M].:.2001.[5]MertzJ,MoreauxL.[J].,2001,196:3252330().[6]GeorgiouE,TheodossiouT,HovhannisyanV,.,,I[J],,2000,176:2532260().[7]GauderonR,LukinsPB,SheppardCJR.[J].,2001,32(7):6912700().©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:7[8]ChuShi2wei,LiuTzu2ming,SunChi2kuang,.2GHz:[J].,2003,11(8):9332938().[9]VolodymyrNikolenko,BoazNemet,RafaelYuste.[J].,2003,30(1):3215().[10]FreundI,DeutschM,SprecherA..,,[J].,1986,50(4):6932712().[11]MengHan,LanderZickler,GuenterGiese,.[J].,2004,9(4):7602766().[12]YiJ,IvanVTomov,YiminW,.[J].,2005,86:1339012133903().[13]TakeshiYasui,YoshiyukiTohno,TsutomuAraki.[J].,2004,9(2):2592264().[14],,,.[J],,2004,31(1):83288[15]GuyCox,EleanorKable,AllanJones,.[J].,2003,141(1):53262().[16]PaulJCampagnola,AndrewCMillard,MarkTerasaki,.[J].,2001,81(1):4932508().[17]BouevitchO,LewisA,PinevskyI,.[J].,1993,65(2):6722679().[18]AndrewCMillard,LeiJ,AaronLewis,.[J].,2003,28(14):122121223().[19]DaneilADombeck,MireilleBlanchard2desce,WattWWebb.[J].,2004,24(4):99921003().[20]AndrewCMillard,LeiJ,Mei2deWei,.[J].,2004,86(2):116921176().[21]GuyCox,NunoMoreno,Josfeij.[J].,2005,10(2):024013().[22]YiciGuo,HowardESavage,FengLiu,.[J].,1999,96(19):10854210856().[23]AlvinTYeh,BunshoKao,WoongGyuJung,.[J].,2004,9(2):2482253().[24]WarrenRZipfel,RebeccaMWilliams,RichardChristie,.[J].,2003,100(12):707527080().[25]AikateriniZoumi,XiaoL,GhassanSKassab,.[J].,2004,87(5):277822786().[26]PatrickStoller,Beop2MinKim,AlexanderMRubenchik,.[J].,2002,7(2):2052214().[27]PaulJCampagnola,LeslieMLoew.[J].,2003,21(11):135621360().[28]./[EB/OL].[2005207210]http:lasie1ap1eng1osaka2u1ac1jp/res_shg1().[29]AndrewCMillard,MarkTerasaki,LeslieMLoew.[J].,2005,88:L462L48().[30]ThierryBoulesteix,EmmanuelBeaurepaire,Martin2PierreSauviat,.:20nm[J].,2004,29(17):203122033().©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(2006)01200082EASecondharmonicgenerationimaginganditsapplicationsinbiomedicineQUJun2le1,CHENDan2ni1,YANGJian2jun2,XUGai2xia1,LINZi2yang1,LIULi2xin1,andNIUHan2ben11)InstituteofOptoelectronicsShenzhenUniversityShenzhen518060P.R.China2)InstituteofModernOpticsNankaiUniversityTianjin3000