亚麻炭疽病病原菌生物学特性的研究

黑龙江农业科学2010(11):57~59HeilongjiangAgriculturalSciences植物保护1,1,2,2,1,1,1,1,1(1.黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086;2.东北农业大学生命科学院,黑龙江哈尔滨150030):对亚麻炭疽病病原菌(Colletotrichumlini)进行了生物学特性研究结果表明:温度pH光照对其生长都有一定的影响,菌丝最适生长温度为25;最适pH为8.0;持续黑暗条件下菌丝生长最好,光照不利于其生长;该菌在供试6种培养基中,在PSA上生长状况最好;碳氮源对菌丝生长有一定的影响,碳源以山梨醇和蔗糖最适合;氮源以蛋白胨和牛肉膏最适合:亚麻炭疽病;亚麻毛盘孢菌;生物学特性:S435.63:A:10022767(2010)11005703:20100806:(1979),,,,,Email:[e-mail](ColletotrichumLinicolumPethybretLaff),[1],,10%~30%,30%,,,[2],11.8%[3],,1材料与方法1.1,,5mm,70%5s,0.1%2min,4,PSA,2848h,PSA[4]1.21.2.1温度对菌丝生长的影响试验05cm7dPDA,510152025303540,5d![5],3122pH对菌丝生长的影响02mol∀L1Na2HPO3∀2H2O01mol∀L1,1mol∀L1NaOH1mol∀L1HClpH23456789101110PDA,,255d1.2.3光照对菌丝生长的影响PDA,(,MT3095)12h,255d1.2.4培养基对菌丝生长和产孢量及孢子萌发的影响(PDA)(PSA)(PMA)(Crapek)(Martin)(Sabourauds),,255d1.2.5碳源对菌丝生长和产孢量及孢子萌发的影响Crapek(NaNO32gK2HPO41gKCl0.5gMgSO40.5gFeSO40.01g30g15~20g1000mLpH)[7],D,,(CK1),,255d1.2.6氮源对菌丝生长和产孢量及孢子萌发的影响Crapek,57植物保护黑龙江农业科学11期L(LAsn)L(LCys)DL(DLPhe)L(LHis)L(LLys),,(CK2),,255d1.3:/cm=/cm-/cm;DPSv7.05[8]2结果与分析2.1PDA,,10~35,25,,20,540(1)1//cmLSD0.05LSD0.0150dD-100.185dD-152.268cC+++203.710bB+++254.642aA+++302.433cC++++350.258dD++++400dD-:+++,++,+,-2.2pHpH2~11,pH8,,6.045cm,,pH7,5.067cm(2)2pHpH/cmLSD0.05LSD0.0123.390eDE++33.488deDE++43.805cdeCDE++++54.130cdCD++++64.218cdBCD++++75.067bB++++86.045aA++++95.043bB++++104.430bcBC++++113.222eE++2.3,2445cm,,,,(3)3/cmLSD0.05LSD0.010.825cC+++2.445aA+++1.480bB+++2.4PMAPSASabourauds3,PSA,6.417cm,PMASabourauds(4)4/cmLSD0.05LSD0.01PDA4.228dD++++PSA6.417aA++++PMA5.733bB+++Crapek4.787cC++++Martin1.652eE++++Sabouraud`s5.697bB+++2.59,D,CK1,(5)5/cmLSD0.05LSD0.014.243abAB++++3.895bcBC++++2.828dE++++D2.807dE++++4.192abAB++++4.540aA+++2.912dDE++++4.253abAB++++3.547cCD++++CK10.500eF-5811期宋喜霞等:亚麻炭疽病病原菌生物学特性的研究植物保护2.69,,6285cm,6/cmLSD0.05LSD0.013.635cC++++2.808dD+++5.575bB+++6.285aA++L3.603cC++++L1.837eE++++DL2.668dD+++L2.617dD++++L1.895eE++++CK22.910dD+3结论与讨论,10~35,25,;pH2~11,pH8;,;6,PSA,PMASabourauds;,;,,,,,,pH6,:[1].[J].,2003(2):1314.[2],,,.[J].,2002(4):2630.[3].[J].,2009(2):137139.[4].[M].:,1998:56.[5],,,.[J].,2007,38(3):317321.[6],.DPS:[M].:,2007.ResearchonBiologicalCharacteristicsofPathogenFlaxColletotrichumLiniSONGXixia1,GUANFengzhi1,PANHong2,WANGLiqun2,HUANGWengong1,KANGQinghua1,ZHAODongsheng1,JIANGWeidong1,LIUYan1(1.IndustrialCropsInstituteofHeilongjiangAcademyofAgriculturalSciences,Harbin,Heilongjiang150086;2.LifeSciencesCollegeofNortheastAgriculturalUniversity,Harbin,Heilongjiang150030)Abstract:ThebiologicalcharacteristicsofthepathogenofflaxColletotrichumdisease(ColletotrichumLini)werestudied.Theresultsshowedthattemperature,pHandlightaffectedthemycelialgrowth;theoptimumgrowthtemperaturewas25;thesuitablepHwas8;itgrewthebestunderconstantlydarkconditions,lightwasnotconducivetothegrowthofmycelium;themyceliagrewfastestinthePSAamongthesixtestingculturemediums;bothcarbonandnitrogensourceshadaneffectonmycelialgrowth,thegrowthofmyceliawasthefastestwithsorbitolandsucrose;beefextractandpeptonewerethemostsuitableforthegrowthofmycelia.Keywords:flaxColletotrichumdisease;Colletotrichumlini;biologicalcharacteristics59