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(HRT)(wash-out)SEM()(carriermaterial)2.12.1.12.1.20.1NHClpH3~4240.1NNaOHpH72.1.3CSTR(Clostridium)2.250ml2%(w/v)(sodiumalginate)2%(w/v)2%(w/v)()0.1MCaCl213~5mm()/152.3()()250ml1atm(35100rpm)2.42.4.110cm2.4.2(sucrose)(20,000mgCOD/L)2.52.5.19800(TCD)20ml12050(10)TCD1402.5.2SIMAZUGC14-B(FID)210110(30)FID1902.5.33.1(HBu)(HAc)(HPr)Ca2+Ni2+3.36.8mmol/lh13mmol/lh8.6mmol/lh20.3mmol/lh19.8mmol/lh19.8mmol/lh21.3mmol/lh18mmol/lh3.4(SEM)?(SEM)120.3mmol/lh19.8mmol/lh6.8mmol/lh2SEM21.Chang,J.-S.,Lee,K.-S.,andLin,P.-J.,“Biohydrogenproductionwithbiofilmprocesses”,Int.J.HydrogenEnergy,Accepted(2002).2.Chen,C.-C.,Lin,C-Y.andChang,J-S.,“Kineticsofhydrogenationproductionwithcontinuousanaerobicculturesutilizingsucroseasthelimitingsubstrate”,Appl.Microbiol.Biotechnol.57,56-64(2001).3.1-6820014.1-7120015.Abdul,R.M.,Yoshinori,F.,Yutaka,N.,Kakizono,T.,Naomiohi,N.,“EnhancedhydrogenproductioninalteredmixedacidfermentationofglucosebyEnterobacteraerogenes”,J.Fermen,Bioeng.83(4),358-363(1997).HBumgCOD/LContainedCa2+(5.0mg/L)6.YokoiH.,TokushigeT.,HiroseJ.,HayashiS.,andTakasakiY.,“HydrogenproductionbyimmobilizedcellsofaciduricEnterobacteraerogenesHO39,J.Ferment.Bioeng.,835,pp.4814841997.7.1–818.NarendraKumar,DebabrataDas,“ElectronmicroscopyofproducingimmobilizedE.cloacaeΙΙT-BT08onnaturalpolymers”,Int.J.HydrogenEnergy,26,pp.1155-1163(2001).Time(h)0510152025VFA(mgCOD/L)0500100015002000250030003500GasGeneration(ml)050100150200250300350CumulativeGasGeneration(ml)CumulativeHydrogenGeneration(ml)HPrmgCOD/LHAcmgCOD/LEtOHmgCOD/LTime(hr)0510152025CumulativeHydrogenGeneration(ml)020406080100120140160CintainedCa2+(0.5mg/L)SuspendedcellsContainedCa2+(50mg/L)3Time(hr)0510152025CumulativeHydrogenGeneration(ml)020406080100120140160180ContainedNi2+(0.32mg/L)ContainedNi2+(32mg/L)SuspendedcellsContainedNi2+(3.2mg/L)(mg/L)Sucrose17,800NH4HCO35,240NaHCO36,720K2HPO4125MgCl26H2O100MnSO45H2O15FeSO47H2O25CuSO45H2O5CoCl25H2O0.125(ml)(ml)/(mmol/lh)3601350.386.8CA275770.282.8CA+AC()240540.233.6CA+TiO22801300.4613CA+AC()3201060.334.3CA+Al2O34602030.4420.3CA+CB210860.418.6CA+CH2301060.467.1CA+AC+CH4201810.4317.8CA+CH+Al2O34401940.4412.9CA+CH+TiO24502130.4721.3CA+AC+Al2O34101680.4116CA+AC+TiO24201800.4318CA+AC+CB4471760.397.6CA+CB+TiO24502040.4519.8CA+Al2O3+CB+TiO22601190.4611.9CA+Al2O3+CH+TiO24602130.4614.2CAAC()CBCH4