利用可生物降解聚合物为碳源和生物膜载体脱氮及其动力学特性研究

31820108ENVIRONMENTALSCIENCEVol.31No.8Aug.2010*201306BDPsPBS.29±1℃NO－3-N25～334mg/LNO－3-N0.107～1.098kg/m3·d.1.098kg/m3·dNO－3-N0.577kg/m3·d.NO－3-NNO－3-N.PBS.EckenfeldernKNO－3-N.SPSS16.0p0.05p=0.5530.05p=0.6320.05.X703.1A0250-3301201008-1839-072009-10-242009-12-252006BAD03B06S307011984～[e-mail]*[e-mail]ticCharacteristicsUsingBiodegradablePolymersasCarbonSourceandBiofilmCarrierLAICai-shengTANHong-xinLUOGuo-zhiRUANYun-jieZHOUWeiSUNDa-chuanCollegeofFisheriesandLifeScienceShanghaiOceanUniversityShanghai201306ChinaAbstractThePBSmaterialthatintheformofinsolublebiodegradablepolymerspelletswasinvestigatedasthesolidcarbonsourceandthebiofilmcarrierfornitrateremovalfromwastewater.Thedenitrificationofnitrateremovalandkineticprocesswerecarriedoutinapacked-bedreactorinordertoremovenitrateinrecirculationaquaculturesystem.Theexperimentalresultsindicatedthattheoptimalinfluentloadingratewasintherangeof0.107-1.098kg/m3·dwhenthewatertemperaturewas29±1℃andtheinfluentnitrateconcentrationwasintherangeof25-334mg/L.Themaximumnitratevolumetricremovalrateof0.577kg/m3·dwasachievedattheinfluentloadingrateof1.098kg/m3·d.Whentheinfluentloadingrateexceeded1.098kg/m3·dthenitratevolumetricremovalratewasdeclined.ThekineticexperimentalresultsshowthatthedenitrificationrateofPBSasthesolidcarbonsourceandthebiofilmcarriercorrespondstofirst-orderkinetics.BasedonthekineticscharacteristicsconstantsnandKusedinEckenfeldermodelwerededucedwhichcanbesuccessfullyappliedforthepredictionofeffluentnitrateconcentration.Thetwogroups’predictivevaluesandactualvalueswereanalyzedbyusingSPSS16.0softwareforPaired-Samplesttestanalysis.ThePaired-Samplesttestanalysisindicatesthatthecorrespondingp0.05valuesare0.553and0.632whichprovedthatnosignificantdifferencesexistbetweenthepredictivevaluesandactualvaluesofthemodel.KeywordsnitratecarbonsourcedenitrificationbiodegradablepolymerskineticsmodelrecirculationaquaculturesystemRAS.NO－3-N400～500mg/L12.NO－3-N3～5.67.50mg/L8.9NO－3-NpH.10～13.NO－3-N31、NO－3-N14.biodegradablepolymersBDPs15～17BDPs.BDPspHDO18.BDPs171920.BDPsPBSPBS.11.1PBSPBSPBS2mm×3mm×4mm.1..500mLPBS150mg/L10mg/L1500mg/L.70r/min29±1℃.2.PBS.1PBSTable1CharactersofPBSforexperiment/℃/MPa/%/%/%1.1589283000.1401.21.PVC.60cm10cmPBS2.747L35cm.PBS..29±1℃pH7.35±0.2NO－3-NKNO325～334mg/LK2HPO410mg/LMgCl2·6H2O200mg/LFeSO4·7H2O10mg/LCaCl2·2H2O10mg/L.1.310%.NO－3-N150mg/LHRT6h.NO－3-NNO－2-N13NO－3-N5%.1Fig.1Flowdiagramoftheexperiment1.4NO－3-N50、150、300mg/LHRTHRTNO－3-N、NO－2-N.NO－3-N25～334mg/LHRT5h2hNO－3-NNO－3-N、NO－3-NNO－2-N.048181.5NO－3-NSPSS16.0.1.60.45μm.21.pH、DOYSI556MPS.22.12Fig.2EffectsofHRTondenitrificationperformance3NO－3-N50、150、300mg/LHRT0.2～9.1h.HRT2HRTHRT.HRT0.8h360.1%、25.0%15.3%HRT3.HRTHRT2.5、4.66.1h90%.HRT.Volokita100.5～1.5m/d0.5m/d1.5m/d3.22HRT.2.23Fig.3EffectsofinfluentloadingratesondenitrificationperformanceandeffluentNO－2-Nconcentration3aNO－3-N25～334mg/LHRT5hPBSNO－3-N.NO－3-N80%..3bHRT2hNO－3-NNO－3-NNO－3-N.NO－3-NNO－3-NNO－3-N.NO－3-N1.098kg/m3·dNO－3-N148131NO－3-NNO－3-N1.098kg/m3·dNO－3-N0.577kg/m3·dNO－3-N.PBS.NO－3-N.3c1.394kg/m3·d1mg/LPBSNO－3-N.NO－3-N0.577kg/m3·d1823PBS0.700mg/g·dPBS0.7NO－3-N0.550kg/m3·dPHB、PCL、Bionolle、、24.BDPsBDPs、.33.1PBS.25.PBS.1/2.1/2.PBS.V·cZ+A·ΔZ·r=V·cZ+ΔZ+A·ΔZ·cZt1Vm3/hcZZNO－3-Nkg/m3Am2ΔZmrkg/m3·h.Δc=cZ+ΔZ－cZV·cZ－V·cZ+ΔZA·ΔZ=－V·ΔcA·ΔZ=cθ－cθ+r=ct2NO－3-Nc/t=0dcdθ=r31r=－kc=－k·θ+cincout=－k·HRT+cin42r=kcc=cin·exp－k·θcout=cin·exp－k·HRT531/2r=k1/2·c1/2c1/2=12k1/2·θ+c1/2inc1/2out=－12k1/2·HRT+c1/2in6cinNO－3-Nkg/m3kcθNO－3-Nkg/m3coutNO－3-Nkg/m3θ、HRTh.4NO－3-N50、150、300mg/LNO－3-N24818c=cin·exp－k·θc=cin·exp－k·θ+KPBS.4Fig.4CorrespondingrelationshipbetweentheeffluentNO－3-NconcentrationandHRTfordifferentinfluentNO－3-Nconcentrations3.2PBS.Eckenfelder72526..Eckenfelder①5Fig.5CurveofkineticofplugflowreactorfordifferentinfluentNO－3-Nconcentrations②.SeS0=e－KD/Ln7S0mg/LSemg/LDmLm3/m2·minKmin－1n.7lnSeS0=－KDLn8348131L=－KDlnSeS09710ln－lnSeS()[]0=－nlnL+lnKD1010Se、S0、DLln－lnSe/S0－lnLnK.50、150、300mg/L3NO－3-NNO－3-NEckenfelder.5－lnLln－lnSe/S0n0.9799～0.9887.ln－lnSe/S0=0lnKD=nlnL11K=enlnLD12212nK0.0022～0.0060min－1.2EckenfeldernKTable2ConstantsnandKofEckenfeldermodelforplugflowreactorNO－3-N/mg·L－1nxnlnLenlnLK500.97996.1541－6.15410.002120.00601500.98156.8542－6.85420.001050.00303000.98877.1390－7.13900.000790.002246.2NO－3-N229℃±1℃pH7.41～7.69DO2.8～4.2mg/LNO－3-N86mg/L145mg/L.SAS16.0Paired-SamplestTest2p0.5530.6320.052.6Fig.6RelationshipbetweenactualandcalculatedeffluentNO－3-Nconcentrations.51PBSNO－3-NHRTNO－3-NNO－3-NHRT.229±1℃NO－3-N25～334mg/L.NO－3-N1.098kg/m3·dNO－3-N0.557kg/m3·d.3PBSNO－3-N50～300mg/L.Eckenfeldern0.9799～0.9987K0.0022～0.0060min－1.4.44818.1OtteGRosenthalH.Managementofclosedbrackish-watersystemforhighdensityfishculturebybiologicalandchemicalwatertreatmentJ.Aquaculture197918169-181.2HondaHWatanabaYKikuchiKetal.HighdensityrearingofJapaneseFlounderParalichthysolivaceuswithaclosedseawaterrecirculationsystemequippedwithadenitrificationunitJ.Suisanzoshoku19934119-26.3MuirPRSuttonDCOwensL.NitratetoxicitytoPenaeus