pBI121质粒图谱及载体信息

pBI121质粒图谱及载体信息启动子：35S终止子：Nos%t+f!b8G%s报告基因：GUS抗性基因：NptIIpBI121载体信息：1R+Yc*n${:X6RLOCUSAY7812968196bpDNAlinearSYN10-NOV-2004/X,_2X:K2P%M4G-w#RDEFINITIONExpressionvectorpBI121_proT-DNAregion,completesequence.ACCESSIONAY781296;D$h$R9@JVERSIONAY781296.1GI:55418080-O,V2p5P:h7c2s+GKEYWORDS.SOURCEExpressionvectorpBI121_pro9x5Y0z(o;l5PORGANISMExpressionvectorpBI121_proothersequences;artificialsequences;vectors.7z3f!V4Y-vtREFERENCE1(bases1to8196)!yP&D9`H'j#Vi9YAUTHORSZeng,Y.H.,Cao,J.W.,Cai,H.Y.,Chen,X.H.andMiao,L.X.TITLEConstructionoftheplantexpressionvectoroftheproBAgenefrom3x-N'}$?.d4t7T&gG2v*b5\BacillussubtilismutanttoprolineanalogandanalysisofthesalttoleranceoftransgenicArabidopsisthalianaJOURNALUnpublishedREFERENCE2(bases1to8196)AUTHORSZeng,Y.H.,Cao,J.W.,Cai,H.Y.,Chen,X.H.andMiao,L.X.?&qx*X9i1J6v*r*I!uTITLEDirectSubmission7_$W$O/U6D)aJOURNALSubmitted(14-OCT-2004)CollegeofLifeScience,WuhanUniversity,/V[)u%[$\7S%u+T,T-}BayiRoad,Wuhan,Hubei430072,P.R.ChinaFEATURESLocation/Qualifiers#b2\/L0aJ6v:M*W&P;t5Ksource1..8196/organism=ExpressionvectorpBI121_pro/mol_type=otherDNA3?!C%}7U:Q3X+N/db_xref=taxon:2994645\&K,\+_/_2pmisc_feature1..306/note=containsT-DNArightborderandNospromotergene318..1112#Mr+^6r&c'^c/gene=NptII6X$d9vH:z/^!_8\PCDS318..1112/gene=NptII6l'c&o'o3h;E1C/codon_start=1/transl_table=11/product=neomycinphosphotransferaseII/protein_id=AAV51261.1/db_xref=GI:55418081q9z3H&q-u3Z-[-kM/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR4B1C8l5A8B4A/K-dPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE0|4a'u.n6M9y7E$L4ZEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY$Ad$T1c-}&BQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF;R1\x-@/[(M0@,`)D;F*lterminator1113..1365-B:I$j+D8a4F0m'f/note=Nospromoter2059..2859$~;C-U3k*S7s/note=CaMV35Smisc_feature2872..3174/note=Arabidopsisthalianap5csBintroninGenBankAccessionNumberX86778gene3175..4287G!|2~:i/r4d)|3L/gene=proBCDS3175..4287&q'F4O1V6|,b,i+Y/gene=proB:s/|(z,v)S:r)F:Qs#~$S;|/codon_start=1;O5P+{9Q+X/transl_table=11)o-z,a6e9H3h+t/product=gamma-glutamylkinase/protein_id=AAV51262.1/db_xref=GI:55418082&yj*[(L5l%I7P/translation=MKKQRIVVKIGSSSLTNSNGSIDEAKIKEHTEAISLLKENGHEV@9O8]/`,@ILITSGAVAAGFSALGYPARPVTIKGKQAAAAVGQTLLMQQYMDHLKRYGLTPAQILL)XT+p'V:})i$U,A#fTRNDFSKRERYRNAYATVMELLERGVVPIINENDSTSVEELTFGDNDMLSALVSGLIH(c4}e7P1MADQLMILTDINGLYDANPNENPDAKRFDYLTDITPELLGYAGSAGSKVGTGGMKSKFL-A6{&f5q&p*@%U0[4J{AAQTALSLGVKVFIGTGAGREKLKVILDGKGDGTYIGDKELSTVNNTRQWIMFHSPISGEIIIDGGAEQAMIHNGSSLLPAGVADVCGSFPKGAVVEVRGPGGIIGKGQTHYSSDEu4J)P(X5K%g%}ILEAKGKRSDQLPGAKQVEVIHRNDWVNLFDEGENKmisc_feature4300..4602&q:~/M*c5K7Y3N2j/note=Arabidopsisthalianap5csBintroninGenBankAccessionNumberX86778/I&oZ%A#B.u1ogene4603..5733/gene=proA'Y9Y#H7{;W0^CDS4603..5733/gene=proA7h;Y8B.d%?,a/codon_start=1n*~%U2v#_(t6^J'X.E/transl_table=11/product=glu-5-semialdehyde!j2K#_3o'x%N0X(n!Q;_!M/protein_id=AAV51263.1#\&~;J'Y(I9q#^5D:@:l&C/db_xref=GI:55418083/translation=MSEVLQKAALAKEAAAEMVMKTTAEKNEALQCIADGLRNERQLILTENQKDIEAGQKRGLTPDIIDRLTLDEKRLLDIADAVELLIGLEDPVGESLETIQKE8{1P0I(u:i#O6ENGLSIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSVMKRALGLSKLPIDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDLVVRESTVP&z0m9]&i!j7R0F(\+m#CVLETGAGNCHVYIDESADPDMARDVVINAKTQRPSVCNAIESLLIHEKWAEVHGRKLL!\2g&W5z8W+YNQLTEKGVELRGDQMICELEPNAKQAEEADWGAEYLAPILSVKTVHDVQEAVRHIQQYGTNHSEAILTENGEHAAYFQTAVDAAAVYHNASTRFTDGFEFgene5758..7569/gene=GUS*C'}/W#x!Y2mCDS5758..7569-o6t(Y5W0K*^8FH/gene=GUS8T3{)j8P'e4?(s/codon_start=18l6`4g4M*f0o4i:E/transl_table=11/product=beta-glucuronidase#\*v1k5g.k&H7f,P/protein_id=AAV51264.1/db_xref=GI:554180849y*f8h6q9c/P/R%l/translation=MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA^6k3SH-^I+tj7S:SIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ3o.f!y2Ax/Z4p,|5}IEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY8g:k(J3],X!|3F4x;nx2c4hFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAU|-H2K3?(U+HEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD.T0Y-u4l#l!c%^AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD%W&c(G,p+}%[6ZTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN*A&r.|;d-q2q7m6TKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ3s)y.P-{,K1z5]7_)Vterminator7630..7882/note=Nosmisc_feature7883..8196/note=T-DNAleftborder2X'c:H7D1t6]ORIGIN1aaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcggagaatta61agggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaact&l'j9w}!_;Q$s,\0w121gacagaaccgcaacgattgaaggagccactcagccgcgggtttctggagtttaatgagct9V&L6`3a'[1I&~181aagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcactatcagc241tagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggtatcc301aattaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgctt361gggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccg421ccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccg)b&h1