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3452007JOURNALOFBEIJINGUNIVERSITYOFCHEMICALTECHNOLOGYVol.34,No.52007(,430074):Novozyme435,51,Novozyme4353%(),40%(),50240r/min24h,9512%1090%,,:;;;:TQ64513:2007205216:863(2006AA020203):,1976,,E2mail:huangying@mail.hust.edu.cn,,,,[122],,70%90%,,[326][7],,,,,,,,,,,[829]Novozyme435,,1111(Novozyme435),NovoNordisk;;,Sigma,11211211,50mL,,(240r/min),11212,,,11213GB/T173761998(M)(SV)(AV),GB55301985100L,5L,295L,300L(),,1L:GC9790(),Innowax,FID,,180,2min3/min230,1min230280y(%):y=m1m100%,m1;m221101459[mg(KOH)/g()],198112[mg(KOH)/g()],,GB/T1737619989912%,,1Fig.1Effectoflipaseontransesterification212,,(1)1,Novozyme4353%(),,,,213,31,[10]4127g,40%(),3%Novozyme435(),240r/min,5024h,,251;51,,,,2Fig.2Effectofmethanol/oilmolarratioontransesterification3Fig.3Effectoftemperatureontransesterification214,,,,4127g,40%(),3%Novozyme435(),51,240r/min,24h,(3)3,500552007215(logP=018),,,,,,;,,10%60%():4127g,3%()Novozyme435,51,240r/min,5024h,(4)40%,92%4Fig.4Effectofamountoftert2butanolontransesterification2162,,,,10%(),,15%();,52,;,10%();,a;b5Fig.5Effectofamountofwaterontransesterification217,,4127g,40%(),3%()Novozyme435,51,240r/min,50,(6)6,24h,24h,6Fig.6Timecourseofthetransesterification218,,(7)7,,10,,,,1555:7Fig.7Operationalstabilityoflipaseintert2butanolmedium:[1]NELSONLA,FOGLIATA,MARMERWN.Li2pase2catalyzedproductionofbiodiesel[J].JournaloftheAmericanOilChemistsSociety,1996,73:1191-1195.[2]FUKUDAH,KONDOA,NODAH.Biodieselfuelpro2ductionbytransesterificationofoils[J].JournalofBio2scienceandBioengineering,2001,92:405-416.[3]NOUREDDINIH,GAOX,PHILKANARS.Immobi2lizedPseudomonascepacialipaseforbiodieselfuelproduc2tionfromsoybeanoil[J].BioresourceTechnology,2005,96:769-777.[4]YOMIW,PRAPHANP,TOSHIHIRON,etal.Con2versionofacidoilby2producedinvegetableoilrefiningtobiodieselfuelbyimmobilizedCandidaantarcticalipase[J].JournalofMolecularCatalysisB:Enzymatic,2007,44:99-105.[5]LILingli,DUWei,LIUDehua,etal.Lipase2catalyzedtransesterificationofrapeseedoilsforbiodieselproductionwithanovelorganicsolventasthereactionmedium[J].JournalofMolecularCatalysisB:Enzymatic,2006,43:58-62.[6]AL2WIDYANMI,AL2SHYOUKHAO.Experimentalevaluationofthetransesterificationofwastepalmoilintobiodiesel[J].BioresourceTechnology,2002,85:253-256.[7]TSAIWentien,LINChihchung,YEHChingwei.AnanalysisofbiodieselfuelfromwasteedibleoilinTaiwan[J].RenewableandSustainableEnergyReviews,2007,11:838-857.[8]HSUAF,JONESK,MARMERWN,etal.Produc2tionofalkylestersfromtallowandgreaseusinglipaseim2mobilizedinaphyllosilicatesol2gel[J].JournaloftheAmericanOilChemistsSociety,2001,78:585-588.[9]NELSONRG,SCHROCKMD.Energeticandeco2nomicfeasibilityassociatedwiththeproduction,process2ing,andconversionofbeeftallowtoasubstitutedieselfuel[J].BiomassandBioenergy,2006,30:584-591.[10]DUWei,XBYuanyuan,ZENGJing,etal.Novozyme4352catalysedtransesterificationofcrudesoyabeanoilsforbiodieselproductioninasolvent2freemedium[J].BiotechnologyandAppliedBiochemistry,2004,40:187-190.Lipase2catalyzedbiodieselproductionfromlardinatert2butanolreactionmediumHUANGYingZHENGHaiYANYunJun(CollegeofLifeScienceandTechnology,HuazhongUniversityofScienceandTechnology,WuhanHubei430074,China)Abstract:AnewmethodforbiodieselproductionfromlardinvolvingcatalysisbyimmobilizedlipaseNovozyme435hasbeendeveloped.Theoptimalparameterswerefoundtobe51molarratioofmethanol/oil,3%(massfraction)Novozyme435,40%(volumefraction)tert2butanol,andareactiontemperatureof50.Theresultsshowedthatafter24hours,thehighestyieldofmethylesterobtainedwas9512%.Whenthelipasewasrepeat2edlyusedfor10cyclesundertheoptimalconditions,yieldsofmethylestersofover90%wereobtained.Thisin2dicatesthatlardcanbeeffectivelyconvertedintobiodieselandthatlipaseexhibitsgoodstabilityunderthecondi2tionsemployed.Keywords:biodiesel;tert2butanol;lard;transesterification2552007