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123003910dcyclophosphamideCTXTHC503.313.2ml/kg10d6.6ml/kgTCYHC503.313.2ml/kgCYEffectofShendanInjectiononImmuneFunctionTreatedwithCyclophosphamideinMiceYaoXiao-MingWangJuanABSTRACT:Objective:Toexploretheeffectofshendaninjectiononimmuneregulationtreatedwithcyclophosphamide(CTX)inmice.Methods:Kunmingmicetreatedwithcyclophosphamide(CTX)tocreatethemiceimmunityfunctionaldisordermodelwereinjectedinthebellycavitywithshendaninjection(3.3to13.2ml/kg)for10days,Themiceimmunityorganweight,thespecialimmuneantibodyofthespleencell,thequantityoftheTlymphocyteandcontentofserumhemolysinHC50inmiceimmunizedwithSRBCwereobserved.Results:afterbeinggivenmedicinewith3.313.2ml/kgfor10days,theweightofthymusglandofthemicewasincreased;The6.6ml/kggroupmice’sspleen’sweight,thenumberofTlymphocyteisremarkablyhigherthantheCTXmodelgroup’s;Femalemiceofthe3.3~13.2ml/kgisremarkablyhigherthantheCTXmodelgrouponhemolysinHC50,butthemalemicedoesn’tincrease.Conclusion:shendaninjectioncouldsignificantlyimprovetheimmunefunctioninmicetreatedwithcyclophosphamide.KEYWORDS:Sendaninjection;Immunefunction;PharmacologicalresearchExperimentalanimalmodel2()())[1],,,[3-5][6][7]IL-2[8]-IgGIgM[9]1.030919230201082000920030901980901980901970526--9705019102079904151999112892090199090197090102072620000602200202189809012000420200004203.10.9%:4.5g500ml12120min42Alsvers:2.05g0.89g0.42g100mlpH7.212120min43HanksNaCl80gMgS047H2O1gMgCl26H2O1g,450mlCaCl21.4g50ml1mlNa2HPO412H2O1.52g,KH2PO40.6g,0.2g10g500ml1ml4Hanks=11186.6104Pa1020min43.5%NaHCO3pH7.251/15mol/LpH7.6KH2PO49.08g1000mlNa2HPO423.882g1000ml13ml87ml1/15mol/LpH7.664%3ml3ml1min4g2mol/L100ml44%400mg10ml72%--2g--100ml2%--4481/15mol/LpH7.689ml6ml2%--2.5mlpH6.491%0.5g50ml9%101%0.5g50ml116mg/ml180mg30ml4121.0g0.05g0.2g1000ml413SRBC=10.2v/v430min2000rpm10min110145630min21SRBC3730min1h42000rpm10min4.12030g011102ChickenredbloodcellCRBC15Alsevers435.JY10001FA20048902220136LXT-YXQ.SG4128AHHS11-4pharmaciaUltrospec3100OlympusGalenTMOlympusLD5-2A51.[2]60610NS6.6ml/kg13.2ml/kg6.6ml/kg3.3ml/kg,CTXipCTXNSip8d910CYCTX60mg/kgNSNS8d910CTXipCTX60mg/kg720%SRBC0.2ml/2.2.1CY2.1.1,60%,CY2.1.230min,CY=mg/10g=mg/10g2.22.2.1BIg---IgMSRBC6SRBCHC502.2.210d1ml1ml10l+2490l2501ml20050010%0.5mlSRBC101ml1103710min2000r/min10min1ml3ml10min540nmODHC50CTXHC50t2ODHC50HC50=SRBC0.25mlSRBC20SRBC/ml4ml10min540nmSRBC2.3-2.3.1T--naph-thylacetateesterase,ANAE---BT2.3.20.2mm30min3712h1%510sTANAE25T4100TCYTT5972%72.42.4.1TSRBCSRBCEErythrocyteEETTETEETETSRBCSRBCETEEaaactive[2]SRBCSRBCSRBC2.4.24d20%SRBC0.2ml/4dHanksHanks1208Hanks210%Hanks9106/ml15mg/ml20.1ml0.1ml1%SRBC0.1ml37530min23500rpm25min0.10.2ml1min1%3SRBC45min500rpm35min0.10.2ml1%3.3.1CTXCTX11CTX81CYmg/10gmg/10g33.342.64**8.060.85**CY25.643.636.160.5130.012.09**6.980.73**29.483.037.071.00*31.182.50**6.980.54**28.823.176.710.55*n=10xsCY*P0.05**P0.011CY051015202530354045cymg/10g******3.2CYHC50CYCY222HC50HC50HC50157.1912.03**165.9816.93**CY19.131.5919.331.6920.282.2357.951.77**19.193.8659.18.58**919.542.1158.437.68**19.231.7452.974.66**n=10xs.CY*p0.05**p0.01p0.012CYHC50050100150200250cyHC50HC50HC50**********##########**3.3-3CYTT6.6mg/kg333CYTT%62.012.66**CY40.36.6256.710.33**48.77.72*54.69.30**46.97.73n=10xsCY*p0.05**p0.013-10ANAE%0102030405060708090cyANAE%*******2.4IgGIgAIgMIgDIgEIgMBTTPHAConATIL-2IL-2RNKLAKCTL11()[2][10],(),,[1113],195712CTXDNADNACTX[14]CTXCTX[15][16]CTXTBBTCTX400ml/kg16hBTCTXBT[14],T[25]CTXBTCTXTTsTCTX[14]CTXPanaxginsengC.A.Mey.SalviamiltiorrhizaBge3.1PanaxginsengC.A.Mey.13“”[17][6][7]IL-2[8]3.2(SalviamiltiorrhizaBge.),“”[17]2030,[18]()[19]14[9][20][21--22]4.1CTXCTXCTXCTXHC50CTXCTX-CYTT6.6mg/kgCTX4.254.3-pHpHpH6.8pH6.4pHTBTB154.4205CY7d541%SRBCSRBC-[3]6%1ml5%(CRBC)0.5ml/231ml1min0.10.2ml35(23)3730minGiemsa510s100200MMCRBCCRBCMM=CRBCM/M=MCRBC/CRBCM16,[23,24]ANAE1.19943472..1994.784-85,106-110,706-7093Wamg,B.K.,Xing,S.T.,Zhou,J.H.,ChineseJ.phar-macol.Toxicol.,1990,4(1):394,,.,1994,17(2):285,,.,1996,12(6):3746..1987,(2):977..1993,9(2):1358..1992,82:2149.[].,1986,(3):36-39.10..,1992,12(7):44344611Wamg,B.K.,Xing,S.T.,Zhou,J.H.,ChineseJ.phar-macol.Toxicol.,1990,4(1):391712,,.,1994,17(2):2813,,.,1996,12(6):37414..199910110915..2..19887210916..1981.39317.1999,Vol.1lNo.418,,,.:.:,2000:1087.19..,1989205-206,217-219,225-23020..1981,(3):16521..1992,(2):5122...1992,(1):923.[J].,1997,13(4):298-301.24,,.[J].,2000,31(4):304.25.1996.230518ANAE19ANAEANAE20MCRBC21CRBCSRBC