市售植物营养剂活性之生物检测

53了量力良量量參pH3.1～9.5度74～5820µS/cm1000µS/cm不不度類精106～103數理度理異精精神蓮12益祿&2諾雷力104數理度略4%103數降20%精丹104數理度24%103數降46%力葉VitaminB力106數理度4%105數降28%104數降73%103數理9%不105104數綠六量量綠54六94年6BioassayforTestingtheEffectsofCommericalPlantNutrientsonCropGrowthYeong-JeneChiangTaiwanAgriculturalChemicalsandToxicSubstancesResearchInstitute,CouncilofAgricultureAbstractCommercialplantnutrientsareusedinordertopromotecropgrowth,producehigheryieldandquality,enhanceresistance,andimprovesoilcharacteristics.Inthesestudies,weevaluatedphytotoxicitypotentialofcommercialplantnutrientsonlocalcrops.Bioassayusedinvolvedplantingpre-germinatedseedsoftestedspeciesinfilterpaperssoakedinthesolutionsoftestedplantnutrientsandmeasuringtheradiclelengthafter5days.1%solutionoftestedplantnutrientsshowedthatpHrangedfrom3.1to9.0,andelectricconductivitylaybetween74and5820uS/cm.Responsesofradicalelongationoftestedcropstoplantnutrientswithdifferentdilutionrevealedmarkeddifference.Itwoulddivideintofivegroupsonthebasisofsuppresseddegreeofradicalelongation.Thefirstgroupexhibitedunapparentdifferenceofradicallengthatanytreatedrateoftestedplantnutrients.Thesecondgrouphadapproximately20%inhibitionat103timesofdilution.Thethirdgrouptreatedwith104and103timesofdilutionwasupto24%and46%reduction,respectively.Thefourthgroupreducedapparentlybyabout28%at105timesofdilution,73%and91%at104and103timesofdilutedrates.Thelastoneexpressedobviouslydifferenttendencytotestedchemicals.Itappearedtherehadaccelerationofradicalelongationat104and105timesofdilution.Withintestedcrops,lettucewasthemostsensitivetotreatedchemicals,mungbeanshowedmoretolerantthanothercrops.Keywordsplantnutrient,bioassay,radicalelongation,growthinhibition.WeedSci.Bull.26(1),June200555類不理理量力(1,7,9)兩(11)不硫類不例料料不來裂量不(11)精精糖S-HFBN-5ALT葉尿(2,11,13)刺量裂度類料(1,2,5)粒粒力度度良理理(2,11)料粒裂降量(11)量參56六94年6料(-稉-5)(綠-5)(-綠)()(-2)料力精精精神丹蓮12葉益祿&2諾VitaminB力雷力量列參Sunderlandetal.(14,15,16)30cm×20cm×濾10粒不數(0103104105106)(100ml/)度25理5度不量不理3mean±S.E.論列pH度數1%pH3.1～9.5力精精神丹12&2諾VitaminB力雷力pH6益祿WeedSci.Bull.26(1),June2005571)Table1.ChemicalcharacteristicsandlabeledapplicationratesoftestedcommercialplantnutrientspH度量(數)7.54760茶500～1000力3.18161000精4.7421類5008.0545800～1200精7.43752000～400010～153.217005.11140類800～10008.5196類800～50006.23350200～1000精4.5655類800～10004.2553神4.81270類500～10003.874400丹4.92440茶800～1500蓮6.417202000～400015.65820500～100024.22170500～10008.11671000～20009.56942000～30008.04750茶200～1500葉7.44430茶100～60004.64731000～30008.476100～1500益祿9.41060800～15007.97352000～4000&24.41550類800～1000諾4.3652500～1000VitaminB力3.28895008.32760雷力5.232501)pH度1%58六94年6pH8精蓮葉pH6.2～7.9()度74～5820µS1000µS神丹蓮12葉益祿&2雷力100µS()pH度離度度離量(8,10,11)不六不類精106～103數理度理異()精精神蓮12益祿&2諾雷力104數理度略4%103數降20%()精丹104數理度24%103數降46%()力葉VitaminB力106數理度4%105數降28%104數降73%103數理9%()不105104數()精丹量量度異()103綠率4657%10544%()精103量理率5356%()理量103綠5754%()丹10537%103率4353%()理量綠10350%WeedSci.Bull.26(1),June200559稉綠不數Fig.1.Effectsofformulatedplantnutrientsontheradicalelongationofdifferenttestedcrops.00.51.01.52.000.51.01.52.000.51.01.500.51.01.500.51.01.500.51.01.5106x105x104x103x106x105x104x103x數精丹%%稉綠稉稉綠綠不數Fig.1.Effectsofformulatedplantnutrientsontheradicalelongationofdifferenttestedcrops.00.51.01.52.000.51.01.52.000.51.01.500.51.01.500.51.01.500.51.01.5106x105x104x103x106x105x104x103x數精丹%%00.51.01.52.000.51.01.52.000.51.01.52.000.51.01.52.000.51.01.500.51.01.500.51.01.500.51.01.500.51.01.500.51.01.500.51.01.500.51.01.5106x105x104x103x106x105x104x103x106x105x104x103x106x105x104x103x106x105x104x103x106x105x104x103x數精丹%%%%()10456%103更74%()力葉VitaminB力量六數降度()103力率55～90%10468%()106數2937%1047360六94年6～94%103更80%()理量36～94%10388～95%()葉不數1)Table2.Effectsofcommercialplantnutrientsontheradicalelongationofdifferenttestedcrops2)1061051041031.15±0.11.16±0.011.17±0.061.03±0.011.12±0.131.06±0.100.96±0.070.80±0.111.1±0.071.00±0.100.76±0.120.54±0.190.96±0.060.72±0.130.27±0.080.09±0.010.851.071.140.731)不數理綠六度理2)類精精精神蓮12益祿&2諾雷力精丹力葉VitaminB力理量33～90%104數55～78%10382～93%率()105～103率48～92%104數29～85%10376～94%率()VitaminB力103數70～86%()力葉VitaminB力度50%量列葉力VitaminB力都104～103WeedSci.Bull.26(1),June200561數50%()類度異量(9,11)料裂糖理(9,11)裂類度(11,12)稉綠不數Fig.2.Effectsofformulatedplantnutrientsontheradicalelongationofdifferenttestedcrops.00.51.01.52.000.51.01.52.000.51.01.50.51.01.500.51.01.500.51.01.5106x105x104x103x106x105x104x103x0力葉VitaminB力數%%稉綠稉稉綠綠不數Fig.2.Effectsofformulatedplantnutrientsontheradicalelongationofdifferenttestedcrops.0.51.01.52.000.51.01.52.0000.51.01.50.51.01.500.51.01.500.51.01.5106x105x104x103x106x105x104x103x0力葉VitaminB力數%%0.51.01.52.00.51.01.52.000.51.01.52.000.51.01.52.000.51.01.52.000000.51.01.50.51.01.50.51.01.50.51.01.500.51.01.500.51.01.500.51.01.500.51.01.5106x105x104x103x106x105x104x103x106x105x104x103x106x105x104x103x106x105x104x103x106x105x104x103x00力葉VitaminB力數%%%%62六94年6力葉VitaminB力105理量30%率異(50%數Table3.Thedilutiontimesofcommercialplantnutrientscaused50%inhibitionoftheradicalelongationoftestedcrops綠力103X104X104X104-103X104-103X104-103X105-104X105X105X104X106-105X106-105X104X105-104X105-104X105X105-104X106-105X葉105-104X105X105-104X