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FDA认可使用的检测系统和试剂盒表1：检测和鉴定食源性病原菌的微量生化复合试剂盒微生物检测系统名称生产商肠杆菌科API-20EaEnterotubeIIaCobasIDAMicro-IDbEnterobac-TeriaceaeIIaBioMerieuxVitekHazelwood,MOHoffmannLaRocheNutley,NJHoffmannLaRocheNutley,NJOrganonTeknikaDurham,NCBectonDickinsonCockeysville,MD李斯特氏菌Micro-IDcAPILidteriaOrganonTeknikaDurham,NCBioMerieuxVitekHazelwood,MOa：采纳为官方最终方法，仅用于沙门氏菌检测。b：采纳为官方最终方法，适用于所有肠杆菌。c：采纳为官方初始方法。表2微生物自动鉴定商品名称分析手段生产商AutomicrobicGNIa,GPIbBiochemicaltests生化试验BioMerieuxVitekHazelwood,MOBactometerConductance电导率BioMerieuxVitekHazelwood,MOATBCarbonassimilation碳源利用BioMerieuxVitekHazelwood,MOVIDASAutomatedEIAc自动酶联BioMerieuxVitekHazelwood,MOMISFattyacidprofile脂肪酸指纹MicrobialIDNewark,DEMaalthusdConductance电导率RadiometerAmericaWestlake,OHSensititreFluorogenicdnzymeReactionsRadiometerAmericaWestlake,OHBiologCarbonutilization碳利用率BiologHayward,CAWalkAwayBiochemical生化BaxterDiagnosticsW.Sacramento,CAAutoSCANBiochemical生化BaxterDiagnosticsW.Sacramento,CAALADINBiochemical生化AnalytabProductsPlainview,NYUniSceptAutoReaderBiochemical生化AnalytabProductsPlainview,NYAutoSceptorBiochemical生化BectonDickinsonCockeysville,MDa：食品中沙门氏菌自动检测系统；AOAC采纳为初始方法。表3核酸杂交分析微生物商品名称检测手段生产厂商Campylobacter弯曲杆菌SNAPGENE-TRAKNon-isotopic非同位素Colorimetric比色法MoloecularBiosystemsSanDiego,CAGENE-TRAKSystemsFramingham,MAEscherichiaColi艾氏大肠杆菌GENE-TRAKColorimetric比色法GENE-TRAKSystemsFramingham,MAListeria李斯特氏菌GENE-TRAKAccuProbebColorimetric比色法Chemiluminescent化学发光法GENE-TRAKSystemsFramingham,MAGEN-PROBESanDiego,CASalmonella沙门氏菌GENE-TRAKGENE-TRAKIsotopicc同位素Colorimetrica比色法GENE-TRAKSystemsFramingham,MAGENE-TRAKSystemsFramingham,MAStaphylococcusAureus金黄色葡萄球菌GENE-TRAKColorimetric比色法GENE-TRAKSystemsFramingham,MAYersiniaGENE-TRAKColorimetric比色法GENE-TRAKSystemsFramingham,MAEnterocolitica肠炎耶尔森氏菌a：AOAC地位：采纳为初始方法。b：用纯培养物鉴定。c：AOAC地位：采纳为最终方法。表4免疫分析微生物毒素商品名称分析手段生产商BacilluscereusDiarrhealEnterotoxin蜡样芽肠肝菌股泻毒素TECRAEIABCET-RPLARPLABioenterprisesRoseville,AustraliaUnipath(Oxod)Ogdensburg,NYCampylobacter弯曲肝菌CampyslideMeritec-campyMicroScreenLALALARPLABectonDickinsonCockeysville,MDMeridianDiagnosticCincinnati,OHMerciaDiagnosticsShalrord,UKClostridiumPerfringensEnterotoxin产气荚膜梭菌肠毒素PET-RPLARPLAUnipath(Oxoid)Ogdensburg,NYEscherichiaColiEHECb0157:H7出血性0157：H7大肠肝菌PetrifilmHECHEC0157EHEC-TEKColineImmuno-blotEIALAEIADipstick-EIA3M,St.Paul,MN3M,CanadaLondon,OntarioUnipath(Oxoid)Ogdensburg,NYOrganon-TeknikaDurham,NCAMPCORCamden,NJLiteria李斯特氏菌Listeria-TEKTECRAc,dAssuranceListertestEIAEIAImmunobeadOrganonTeknikaDurham,NCBioenterprisesRoseville,AustraliaBioControlSystemsBothell,WASalmonella沙门氏菌BactigenSpectateMicroscreenLALALAWampoleLabsCranbury,NJRhone-PoulencGlasgow,UKMerciaDiagnosticsSurrey,UK表4免疫分析（续表）微生物毒素商品名称分析手段生产商WellcolexLALaboratoireWellcomeParis,FranceSerobactLALAREMEL,Lenexa,KSUnipath(Oxoid)Ogdensburg,NY1-2TestImmuno-DiffusionBioControlDystemsBothell,WAPATH-STIKDipstick-EIALUMAC,LandgraafTheNetherlandsSalmonella-TEKd,eEIAOrganonTeknikaDurham,NCTECRAc,fEIABioenterprisesRoseville,AustraliaUniqueDipstick-EIABioenterprisesRoseville,AustraliaEQUATEEIABinax,Portland,MEBacTraceEIAKirkegaardPerryGaithersburg,MDAssurancefELABioControlSystemsBothell,WA志贺氏菌BactigenLAWampoleBabsCranbury,NJWellcolexLALaboratoireWellcomeParis,France金黄色葡萄球菌Staphylo-SlideLABectonDickinsonCockeysville,MD金黄色葡萄球菌肠毒素STE-RPLARPIAUnipath(Oxoid)Ogdensburg,NYTECRAc,dEIABioenterprisesRoseville,AustraliaSET-EIAEIAToxinTechnologyMadison,WIELATransiaLyon,FranceVibrioCholeraeEnterotoxin霉肠肝菌肠毒素VET-RPLAgRPLAUnipath(Oxois)Ogdensburg,NYa：缩写：EIA酶免疫分析；RPLA，反相被动乳胶凝集。LA，乳胶凝集。b：溶血性大肠肝菌。c：也适用于Biopro。d：AOAC地位：采纳为初始方法。e：两种不同增强酸免疫分析的方法：检测所有食品或低水份食品中的沙门氏菌。f：AOAC地位：采纳为最终方法。g：也可检测大肠肝菌耐热毒素。5其它商品化快速检测方法和培养基微生物商品名称分析手段生产商Bacteria细菌RedigelbMedium,APCRCRScientificGoshen,TNIsogridbHGMFQALabsSanDiego,CAEnlitencATPPromegaMadison,WIPetrifilmdDry-film3M,St.Paul,MNCliform/大肠菌群E.coli埃希氏大肠杆菌IsogridbHGMF/MUGQALabsSanDiego,CAPetrifilmdDry-film3M,St.Paul,MNColiQuikeMUG&ONPGHachCompanyAmes,IAColilertcMUG&ONPGEnvironeticsBranford,CTLST-MUGfMPN-MediumDifco,Detroit,Mi&Gibco,GrandIsland,NYColitrakMPN-MUGBiocontrolSystemsBothell,WAColiCom-pletebEnzymaticBiocontrolSystemsBothell,WAE.coli大肠肝菌MUGdiscMUGREMEL,Lenexa,KSSalmonella沙门氏菌IsogriddHGMFQALabsSanDiego,CAOSRTMedium/motilityUnipath(Oxoid)Ogedensburg,NYRambachMediumTechnogramParis,FranceMUCAPC8EsteraseBiolifeMilano,ItalyXLT-4MediumDifco,Detroit,MTYersinia卵尔森氏菌DyebindingCrytalViolet洁晶紫PolysciencesWarrington,PAa：缩写：APC，需氧菌平板计数；HGMF，疏水格栅滤膜；ATP，三磷胶腺苷；MNG，4-甲基伞形酮。ONPG，0-硝基苯005-B-D苷露糖苷。MPN，最大近似值。b：采纳为最终方法。c：应用于牛奶检测。d：采纳为初始方法。e：应用于水质分析。f：用于冷冻或冷藏食品的大肠肝菌分析，采纳为最终方法