新城疫_系病毒不同空斑克隆株生物学测定试验

ND、21952111000CEF1.6%37℃72hφ2~3mm、φ1~2mm、φ＜1mmSPFELD50、MDT、ICPI。。BiometricstestofthedifferentPlaqueclonestraintoNDstrainvirusWANGChunhua(LiaoningYikangBiologicalProductCo.,Ltd.LiaoyangLiaoning111000)Abstract:NDstrainviruswasinoculatedtotheChickenembryofibroblasts(CEF)monolayerbylimitingdilution,thentheCEFmonolayerwascoveredby1.6%agarosewithneutralredandwasculturedtoto72hoursat37℃.Theviruswasharvestedthrouhgrepeatedfreezingandthawingafterthethreedifferentplaqueswerepickedoutaccordingtosizefrombigtosmall(φ2~3mm,φ1~2mmandφ＜1mm).TheviruswasproliferatedthroughSPFchickembryofor3passageswiththesamemethod.ThetestresultoftheELD50,MDTandICPItothelatestharvestvirusshowedthatthevirulenceandthefecundityofthevirusfromthedifferentplaquesweredifferent.Themediumplaquevirulencewasthestrongestandthebigplaqueless,thesmallplaquewastheweakest.Thebigplaquefecunditywasthestrongestandmediumplaqueless,thesmallplaquewastheweakest.Keywords:NDstrainvirus;plaque;virulence;fecundity20107ChinaAnimalHealth11。SPF。11.1。1.21SPF、10SPFSPF。1.3(CEF)10。1.4CEF37℃1h1/300001.6%199-72hφ2~3mm、φ1~2mm、φ＜1mm0.5~1mlPBS-15℃3。。1.510SPF37℃72h2H。1.6(ELD50)10－7~10－910SPF《》2000Reed-MuenchELD50。1.7(MDT)(ICPI)《》2000。22.1MDT、ICPIMDTICPI1。2.2ELD50SPF2。33.1CEFSPF。。。3.2。CEF。■DH
 
!# $%&’
       
 !#$12ChinaAnimalHealth20107